Milliplex map human metabolic hormone magnetic bead panel hmhemag 34k

The concentrations of GLP-1 active, GIP, and amylin total were measured via multiplex immunoanalyses based on xMAP technology using a Milliplex MAP Human Metabolic Hormone Magnetic Bead Panel (HMHEMAG-34K) (Millipore, Billerica, MA, USA) and a Luminex 100 IS analyzer (Luminex Corporation, Austin, TX, USA).

Anthropometric measures and blood pressure: A stadiometer was used to measure height and a periodically calibrated scale accurate to 0.1 kg was used to measure body weight. Waist circumference was measured with a tape measure placed at the midpoint between the lowest rib and the upper part of the iliac bone. Resting blood pressure was measured in a seated position after 5 min of resting, using a digital M6 Comfort monitor (Omron, Kyoto, Japan). Out of three measurements, the first measurement was disregarded, and a mean value was calculated for the remaining two measurements.
Gastrointestinal and appetite hormones. The concentrations of glucagon-like peptide-1 (GLP-1), amylin, and peptide YY (PYY) were measured using a Milliplex MAP Human Metabolic Hormone Magnetic Bead Panel (HMHEMAG-34K) (Millipore, Billerica, MA, USA) and a Luminex 100 IS analyzer (Luminex Corporation, Austin, TX, USA).