Pyrrolidine dithiocarbamate

Manufactured by Bio-Techne

Sourced in United States

Pyrrolidine dithiocarbamate is a chemical compound used in various laboratory applications. It functions as a chelating agent, capable of binding to metal ions. The core purpose of this product is to facilitate the study and manipulation of metal-containing systems in a research environment.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hepg2, by National Collection of Authenticated Cell Cultures (2 mentions) Adrenaline, by Merck Group (1 mentions) Noradrenaline, by Merck Group (1 mentions) Isoprenaline, by Merck Group (1 mentions) Recombinant murine granulocyte macrophage colony stimulating factor rmgm csf, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Pd184352, by Selleck Chemicals (1 mentions) Lps from escherichia coli 0111 b4, by Merck Group (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Sb203580, by Selleck Chemicals (1 mentions) Smmc 7721, by National Collection of Authenticated Cell Cultures (1 mentions) Dulbecco modified eagle medium (dmem), by Solarbio (1 mentions) Hep3b, by National Collection of Authenticated Cell Cultures (1 mentions) Ag1478, by Bio-Techne (1 mentions) β actin sc 1616, by Santa Cruz Biotechnology (1 mentions) 3h glutamic acid, by PerkinElmer (1 mentions)

Close spelling variants of this product

Pyrrolidine dithiocarbamate (PDTC) (5 citations)

3 protocols using pyrrolidine dithiocarbamate

Immunofluorescence Analysis of AANAT Regulation

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The reagents adrenaline, noradrenaline, isoprenaline, propranolol, H-89 (PKA inhibitor), the primary antibodies (anti AANAT: S0564; and anti-phospho AANAT: 0939), the secondary FITC-labelled antibody (S0814), and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI 1640 medium and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island, New York, NY, USA). The NF-κB blockers acetyl-l-leucyl-l-leucyl-l-norleucinal (ALLN) and pyrrolidine dithiocarbamate (PDTC) were purchased from Tocris (Minneapolis, MN, USA). The recombinant murine granulocyte–macrophage colony-stimulating factor (rmGM-CSF) were obtained from Peprotech (Rocky Hill, NJ, USA). All reagents were of analytical grade.

Pires-Lapa M.A., Carvalho-Sousa C.E., Cecon E., Fernandes P.A, & Markus R.P. (2018). β-Adrenoceptors Trigger Melatonin Synthesis in Phagocytes. International Journal of Molecular Sciences, 19(8), 2182.

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Cell Culture Protocol for HBV-related HCC

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HBV-unrelated HCC cell lines (HepG2, SMMC7721 and Huh7) and HBV-related HCC cell lines (HepG2.2.15 and Hep3B) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Solarbio, Shanghai, China) supplemented with penicillin (100 U/mL), streptomycin (0.1 mg/mL), and 10 % fetal bovine serum (Gibco, Grand Island, NY, USA) in a 5 % CO₂ humidified incubator at 37 °C. G418 (6.5 mg/mL; Solarbio, Shanghai, China) was added to the culture medium to maintain HepG2.2.15 cells. LPS from Escherichia coli 0111:B4 was purchased from Sigma Aldrich (St. Louis, MO, USA). SB203580, SP600125, and PD184352 were purchased from Selleckchem (Houston, TX, USA) and pyrrolidine dithiocarbamate (PDTC) from Tocris (Bristol, UK).

Wang Y., Cai J., Zeng X., Chen Y., Yan W., Ouyang Y., Xiao D., Zeng Z., Huang L, & Liu A. (2015). Downregulation of toll-like receptor 4 induces suppressive effects on hepatitis B virus-related hepatocellular carcinoma via ERK1/2 signaling. BMC Cancer, 15, 821.

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Glutamate Transporter Regulation Assay

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RX, MnCl₂ and 3,4,5-dimethyl thiazol-2,5-diphenyl tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO). The cell culture media such as MEM, DMEM and Opti-MEM, and Lipofectamine 2000 were obtained from Invitrogen (Carlsbad, CA). [³H]Glutamic acid was from PerkinElmer. UCPH 101, AG1478, pyrrolidine dithiocarbamate (PDTC), H89, PP2, LY294002 (LY), PD98059 (PD), dihydrokainate (DHK) and G15 were from Tocris Bioscience (Ellisville, MO). GLAST (ab 416) and GLT-1 (ab 58571) antibodies were from Abcam (Cambridge, MA). ERK (sc-135900), phospho-ERK (sc-7383), Akt (sc-55523), phospho-Akt (sc-7985), CREB (sc-186), phospho-CREB (sc-7978), EGFR (sc-120), phospho-EGFR (sc-12357), NF-κB (p65, sc-372 and p50, sc-114), EAAC1 (sc-25658) and β-actin (sc-1616) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). A luciferase reporter assay kit was obtained from Promega (Madison, WI).

Karki P., Webb A., Zerguine A., Choi J., Son D.S, & Lee E. (2014). Mechanism of Raloxifene-induced Upregulation of Glutamate TransporterS in Rat Primary Astrocytes. Glia, 62(8), 1270-1283.

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