The metal contents of the protein samples were determined using a Thermo X Series II ICP-MS instrument (Thermo Fisher Scientific Inc., MA, USA), as previously reported [21 (link)]. The protein concentrations and metal contents were used to calculate the average number of metal atoms loaded per ferritin cage [25 (link)], as reported by Si et al. All samples were assessed at least twice.
Thermo x series 2 icp ms instrument
Manufactured by Thermo Fisher Scientific
Sourced in Germany
The Thermo X Series II ICP-MS instrument is a state-of-the-art analytical tool designed for elemental analysis. It utilizes inductively coupled plasma mass spectrometry (ICP-MS) technology to accurately detect and quantify trace elements in a wide range of sample types.
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3 protocols using thermo x series 2 icp ms instrument
1
Quantifying Metal in Proteins
2
Trace Metal Analysis in Fish Tissue
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Dorsal fillet samples were collected from each specimen. For digestion, 1.5 g of each tissue was placed into a Teflon pressure vessel, and concentrated nitric acid and hydrogen peroxide 30% v/v were added. The samples underwent microwave digestion for mineralisation. After cooling, the digested samples were transferred into 50-ml measuring tubes. The inner walls of the Teflon pressure vessels were rinsed with ultrapure distilled water, and the rinse water was transferred into the tubes. Ultrapure distilled water was then added to the tubes to a final volume of 50 ml. Quantification of copper and tin was performed by a Thermo Xseries II ICP-MS instrument (Thermo Scientific, Germany) equipped with a CETAC ASX 500 Model 520 (CETAC Technologies, USA) auto sampler and a peristaltic pump nebuliser. Accuracy of analysis was tested using certified reference material (NRCC-DORM-2 Dogfish muscle, National Research Council of Canada, Ottawa, Canada). Concentrations measured in reference material fulfil the range of the certified values. LOQ of method was 0.020 mg/kg for both copper and tin.
3
ICP-MS Analysis of Cereals
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A Thermo X series II ICP-MS instrument (Thermo Scientific, Germany), with interface Ni sampler and skimmer cone, was equipped with a CETAC ASX 500 Model 520 (CETAC Technologies, USA) auto sampler and a peristaltic pump nebulizer.
Operating conditions were as follows: forward power 1.40 kW, coolant gas flow rate 13.0 L/min, auxiliary gas flow rate 0.70 L/min, nebulizer gas flow rate 0.90 L/min, dwell time 10 ms, with five replicates. Torch position, ion lenses and gas output were optimized daily with a tuning solution.
The Collision Cell Technique (CCT), performed with a Helium/Hydrogen mixture (95/5) at a flow rate of 3.5 ml/min, was used to remove interferences.
An ETHOS 1 microwave digestion system (Milestone S.r.l, Italy) was used for acid digestion of cereals and reference materials. The recoveries are shown in Table S1. The Limit Of Quantification (LOQ) is the lowest concentration of the analyte that can be correctly quantified in the sample. The percentage of recovery (range 80-120) is determined by dividing the value observed in the analytical procedure by the reference value of the Reference Material (Table S1).
Operating conditions were as follows: forward power 1.40 kW, coolant gas flow rate 13.0 L/min, auxiliary gas flow rate 0.70 L/min, nebulizer gas flow rate 0.90 L/min, dwell time 10 ms, with five replicates. Torch position, ion lenses and gas output were optimized daily with a tuning solution.
The Collision Cell Technique (CCT), performed with a Helium/Hydrogen mixture (95/5) at a flow rate of 3.5 ml/min, was used to remove interferences.
An ETHOS 1 microwave digestion system (Milestone S.r.l, Italy) was used for acid digestion of cereals and reference materials. The recoveries are shown in Table S1. The Limit Of Quantification (LOQ) is the lowest concentration of the analyte that can be correctly quantified in the sample. The percentage of recovery (range 80-120) is determined by dividing the value observed in the analytical procedure by the reference value of the Reference Material (Table S1).
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