Oxidative stress in PC12 cells was reflected by the levels of malondialdehyde (MDA), SOD, glutathione peroxidase (GSH-Px) and catalase (CAT), as detected using a Lipid Peroxidation MDA Assay kit (cat. no. S0131S; Beyotime Institute of Biotechnology), a SOD Activity Assay kit (cat. no. ab65354; Abcam), GSH-Px Assay kit (cat. no. EKC39116; BioVision, Inc.) and a CAT Assay kit Without Hydrogen Peroxide (cat. no. 700910; Cayman Chemical Company), respectively. All operational procedures followed the manufacturer's protocols.
Gsh px assay kit
Manufactured by Abcam
Sourced in United States
The GSH-Px Assay kit is a laboratory product designed to measure the activity of the glutathione peroxidase (GSH-Px) enzyme. GSH-Px is an important antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. The kit provides the necessary reagents and protocols to quantify GSH-Px activity in various biological samples.
Automatically generated - may contain errors
2 protocols using gsh px assay kit
1
Oxidative Stress Evaluation in PC12 Cells
2
Liver Antioxidant Enzyme Activity Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissue lysates were subjected to a GSH assay kit (Cayman Chemical, # 703002, Ann Arbor, MI, USA) and a GSH Px assay kit (Abcam ab102530, USA) according to the manufacturer's instructions as previously described [31] . To determine the manganese superoxide dismutase (MnSOD) activity, aliquots of liver lysates were treated with CuZnSOD inhibitor and then subjected to a commercial superoxide dismutase (SOD) assay kit (Cayman Chemical, # 706002, Ann Arbor, MI, USA) according to the manufacturer's instructions. Catalase activity was measured using a commercial catalase activity assay kit (Cayman Chemical, # 707002, Ann Arbor, MI, USA) according to the manufacturer's instructions as previously described [31] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!