Recombinant loxl2

Manufactured by R&D Systems

Recombinant LOXL2 is a lab reagent produced by R&D Systems. It is a recombinant form of the human lysyl oxidase-like 2 (LOXL2) protein. LOXL2 is an extracellular copper-dependent amine oxidase enzyme that plays a role in the cross-linking of collagen and elastin in the extracellular matrix.

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Lab products found in correlation

Loxl3, by R&D Systems (1 mentions) Clear black plates, by Greiner (1 mentions)

Close spelling variants of this product

Recombinant human LOXL2 LOXL2

3 protocols using recombinant loxl2

Amplex Red Assay for Lysyl Oxidase

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The standard amplex red assay has been used as previously described [14 (link)] using putrescine as a substrate and native ´fibroblast derived LOX, which was prepared by collecting the supernatant of IMR90 cells, followed by a size filtration > 10 kDa for concentration and < 50 kDa to separate from LOXL2, or recombinant LOXL2 as enzyme (R&D Systems).

Chang J., Lucas M.C., Leonte L.E., Garcia-Montolio M., Singh L.B., Findlay A.D., Deodhar M., Foot J.S., Jarolimek W., Timpson P., Erler J.T, & Cox T.R. (2017). Pre-clinical evaluation of small molecule LOXL2 inhibitors in breast cancer. Oncotarget, 8(16), 26066-26078.

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Collagen Crosslinking Enzyme Quantification

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Black µclear plates (Greiner bio one, Austria) were coated with human lung collagen (EPC, Owensville, Missouri, USA) at 4 °C overnight. Plates were washed with PBS once followed by incubation with either 20 nM or 100 nM recombinant LOXL2 or LOXL3 (R&D Systems, Minneapolis, MN) for 2 hours. The collagen reaction was stopped using fixative and free aldehyde residues were fluorescently labeled using the Aldehyde Site Detection Kit (KA1295, Abnova, Germany). FITC fluorescence was measured using EnVision and the respective Software.

Aumiller V., Strobel B., Romeike M., Schuler M., Stierstorfer B.E, & Kreuz S. (2017). Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis. Scientific Reports, 7, 149.

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Quantifying LOX Activity in MSCs

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LOX activity in MSCs in monolayer culture was determined using a LOX activity kit (Abcam, Cambridge, MA). Media samples were collected from well plates and analyzed following the manufacturer’s instructions. The resulting fluorescence was read on a microplate reader (excitation/emission= 540/590 nm) and recombinant LOXL2 (R&D Systems, Minneapolis, MN) was used as a standard.

Mitra D., Yasui O.W., Harvestine J.N., Link J.M., Hu J.C., Athanasiou K.A, & Leach J.K. (2018). Exogenous lysyl oxidase-like 2 and perfusion culture induce collagen crosslink formation in osteogenic grafts. Biotechnology journal, 14(3), e1700763.

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