N pentamethyldiethylenetriamine

The fungal pathogens used in this study are shown in Table 1. The media used in this study were YPD (1% yeast extract [Bioshop, Canada], 2% peptone [Bioshop], 2% dextrose [Bioshop]), PDB (24 g potato dextrose broth [Himedia, India] in 1 L distilled water), RPMI 1640 medium (10.4 g RPMI 1640 powder [Sigma-Aldrich, USA], 34.5 g MOPS [3-(N-morpholino) propanesulfonic acid, Sigma-Aldrich], 2 g dextrose, in 1 L distilled water, with pH adjusted to 7.0 with NaOH), spider medium (10 g nutrient broth [Himedia], 10 g mannitol [Panreac, Spain], 2 g K₂HPO₄, in 1 L distilled water, adjusted to pH 7.2 with H₃PO₄), YNB (0.17% yeast nitrogen base w/o amino acids [Bioshop], 0.5% (NH₄)₂SO_4, 2% dextrose) and modified Dixon medium (36 g malt extract [Merck, Germany], 20 g desiccated oxbile [Sigma-Aldrich], 10 mL Tween 40 [Sigma-Aldrich], 6 g peptone [Bioshop], 2 mL glycerol [Scharlab, Spain], 2 mL oleic acid [Sigma-Aldrich], in 1 L distilled water). All media were solidified by adding 2% agar (Bioshop) if needed, except mDixon medium (1.5% agar).
For synthesis reaction, N, N, N′, N′, N′′-pentamethyldiethylenetriamine [Sigma-Aldrich], alkyl bromides [Sigma-Aldrich], 1, 4-butanesultone [Sigma-Aldrich], and acetonitrile [POCh S. A., Poland] were purchased. All compounds were analytical reagent quality and used without further purification.

Lupranol 2095 (polyether polyol: hydroxyl number 35 mg KOH/g, functionality 3, viscosity (25 °C) 8500 mPa·s), Lupranol 1200 (polypropylene glycol: hydroxyl number 248 mg KOH/g, functionality 2, viscosity (25 °C) 72 mPa·s), Lupranate MI (a mixture of 2,4′- and 4,4′-diphenylmethane di-isocyanate (MDI): NCO-content 33.5 g/100 g, viscosity at 25 °C 12 mPa·s) and Lupranate M20 (MDI: NCO-content 31.5 g/100 g, viscosity at 25 °C 200 mPa·s) were kindly supplied by BASF. DABCO DC198 (by Evonik) is a silicone glycol copolymer that acts as a surfactant that regulates cell sizes. N,N,N′,N″,N″-pentamethyldiethylenetriamine (PMDETA), the amine catalyst, and tin(II) 2-ethylhexanoate and bismuth(III) trifluoromethanesulfonate Bi(OTf)₃, the metal catalysts, were purchased from Sigma Aldrich. The isocyanate index was set at 100.

Paper pulp used in this study were kindly provided by Chung–Hua Paper Inc., Taiwan (LBKP grade). Cupric bromide (CuBr₂, 99%) 4,4–dimethylaminopyridine (DMAP, 99%), 2–bromoisobutyryl bromide (BiB, 97%), 2,2,6,6–tetramethylpiperidin–1–yl)oxyl (TEMPO, 98%), 2,2′–azobis(2–methylpropionitrile) (AIBN, 98%), ethyl 2–bromoisobutyrate (EBiB, 99%), N,N,N’,N´´,N´´–pentamethyldiethylenetriamine (PMDETA, 99%), methyl methacrylate (MMA, 99%), and triethylamine (TEA, 99.5%) were purchased from Sigma–Aldrich (Darmstadt, Germany). Sodium hydroxide (NaOH, 96%), hydrochloric acid solution (HCl_(aq), 35%), sodium bromide (NaBr, 99%), and sodium hypochlorite aqueous (NaClO_(aq), 12%) were purchased from Showa Corporation (Saitama, Japan). Commercial PMMA with average molecular weight (M_n) of 600,000 g/mol was purchased from Polyscience (Niles, IL, USA). All solvents were used without any further purification.