Rabbit anti human lc3a b

For immunofluorescence (IF) staining, hMDM containing chamber slides were fixed in 4% paraformaldehyde. Cells were permeabilized by saponin (Sigma, S7900) and stained with DAPI, a polyclonal rabbit anti-human LC3 Ab (Cell Signaling Technology, 2775S), and an anti-Lm polyclonal Ab obtained from Lm infected Balb/c mice.⁶² (link) For detection, chicken anti-mouse Alexa 488 (A21200) and goat anti-rabbit Alexa Fluor 568 (A11036) were used (Molecular Probes/Invitrogen). Chamber slides were analyzed using a Zeiss Observer, Zeiss LSM7 Live and Axiovision/ZEN 2009 Software (Carl Zeiss, Göttingen, Germany). Quantification of LC3⁺ compartments was assessed by counting > 20 Lm infected cells.

SAL (purity, >99%) was purchased from Chengdu Ruifensi Biotechnology Co., Ltd. RPMI 1640 culture medium was purchased from HyClone (GE Healthcare Life Sciences), while fetal bovine serum was purchased from Zhejiang Tianhang Biotechnology Co., Ltd., and penicillin-streptomycin was purchased from Beyotime Institute of Biotechnology. Cell Counting Kit (CCK)-8 assay kit was purchased from Dojindo Molecular Technologies Inc., while DEX (Chinese medicine standard, H41020036) was purchased from Shanghai Shyndec Pharmaceutical Co., Ltd., and the cell cycle detection kit was purchased from Nanjing KeyGen Biotech Co., Ltd., and the Annexin V-FITC/PI apoptosis kit was purchased from BD Biosciences. The total RNA extraction kit was purchased from Tiangen Biotech Co., Ltd., while the reverse transcription and quantitative PCR (qPCR) kits were purchased from Toyobo Life Science, and the acridine orange stain was purchased from Biotopped Life Sciences. The rabbit anti-human c-Myc and GAPDH antibodies were purchased from ProteinTech Group, Inc., while the rabbit anti-human LC3A/B, Bax, BCL-2 and cleaved PARP antibodies were purchased from Cell Signaling Technology, Inc., and the goat anti-rabbit IgG-HRP antibody was purchased from BIOSS. Lastly, the PCR primers were synthesized by Shanghai Shenggong Biology Engineering Technology Service, Ltd.