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Rat igg isotype control

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The Rat IgG isotype control is a laboratory reagent used as a control in immunoassays involving rat immunoglobulin G (IgG). It serves as a reference to establish baseline signals and assess non-specific binding in experiments where rat IgG is the target.

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Lab products found in correlation

Hrp conjugated goat anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Southern Biotech (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Be0146, by BioXCell (1 mentions) Il 33, by R&D Systems (1 mentions) Il 25, by R&D Systems (1 mentions)

Close spelling variants of this product

Control IgG (149 citations) Control rat IgG (34 citations) Isotype control IgG (14 citations) Isotype IgG (14 citations) Isotype control (8 citations) Rat Isotype Control (2 citations)

5 protocols using rat igg isotype control

1

CD8+ T Cell Depletion Methodology

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CD8+ T cell depletion was performed as previously described [11 (link)]. p210 immunized mice implanted with a subcutaneous osmotic pump to infuse AngII as described above were intraperitoneally injected with 150μg of anti-CD8 antibody (53-6.7; eBioscience, San Diego, CA) or isotype rat IgG control (Sigma-Aldrich) 3 days before the first immunization and then twice a week thereafter until euthanasia. The efficiency of the depletion was assessed by flow cytometry. Immune response to the injected antibody was assessed using ELISA. Briefly, sera from 5 mice per group were pooled and diluted 1:400 based on prior optimization. Capture antigens used were either CD8 Ab or Isotype at a concentration of 20μg/ml. After blocking with 1% BSA for 2 hours, diluted sera were incubated with the capture antigens for 2 hours, washed, and incubated with HRP-conjugated goat anti mouse IgG antibody (1:5000, Thermo Scientific). The ELISA plates were washed and detection chromogen (ABTS, Southern Biotech) was applied until color development and read on a micro-plate reader at 405 nm.
Honjo T., Chyu K.Y., Dimayuga P.C., Lio W.M., Yano J., Trinidad P., Zhao X., Zhou J., Cercek B, & Shah P.K. (2015). Immunization with an ApoB-100 Related Peptide Vaccine Attenuates Angiotensin-II Induced Hypertension and Renal Fibrosis in Mice. PLoS ONE, 10(6), e0131731.
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2

Oncolytic Virus and Checkpoint Inhibitor in Glioblastoma

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C57BL/6 mice were implanted with 005 GSC (1.0×105 cells/mouse), and treated with intratumoral injection of ICOVIR17 (1.6 × 107 PFU/mouse) or PBS on days 13 and 18. Anti-mouse PD-1 antibody (BioXCell, BE0146, clone RMP1–14, 12.5 mg/kg) or isotype rat IgG control (Sigma, l4131, 12.5 mg/kg) was injected intraperitoneally (ip) on days 14, 17 and 19 (4 groups, n=3/group). On day 20, mice were euthanized and tumors were collected. Total RNA was extracted from tumor tissues using RNeasy mini kit (Qiagen, 74104) following the manufacturer’s protocol, and kept at −80 °C until analysis. Gene expression analysis was performed by the NanoString Mouse PanCancer Immune Panel (115000142) and Mouse Myeloid Innate Immunity Panel (115000181). 110 ng/mouse of total RNA was hybridized with reporter codeset and capture probeset following the protocol provided by NanoString. Hybridized samples were prepared using the automated nCounter prep station and analyzed by Set up Digital Analyzer (nCounter Max Analysis System). Data were processed using nSolver analysis software and the nCounter digital analysis module. The housekeeping genes expressions were used for the normalization of the gene expression analysis.
Kiyokawa J., Kawamura Y., Ghouse S.M., Acar S., Barcin E., Martínez-Quintanilla J., Martuza R.L., Alemany R., Rabkin S.D., Shah K, & Wakimoto H. (2020). Modification of extracellular matrix enhances oncolytic adenovirus immunotherapy in glioblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(3), 889-902.
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3

Immune Modulation in Mice

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Mice were injected IP with 1mg GK1.5 (BioXcell, West Lebanon, NH) or Rat IgG isotype control (Sigma, St. Louis, MO) every two weeks for six weeks.
Korn L.L., Thomas H.L., Hubbeling H.G., Spencer S.P., Sinha R., Simkins H.M., Salzman N.H., Bushman F.D, & Laufer T.M. (2014). Conventional CD4+ T cells regulate IL-22 producing intestinal innate lymphoid cells. Mucosal immunology, 7(5), 1045-1057.
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4

Immune Modulation in Mice

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Mice were injected IP with 1mg GK1.5 (BioXcell, West Lebanon, NH) or Rat IgG isotype control (Sigma, St. Louis, MO) every two weeks for six weeks.
Korn L.L., Thomas H.L., Hubbeling H.G., Spencer S.P., Sinha R., Simkins H.M., Salzman N.H., Bushman F.D, & Laufer T.M. (2014). Conventional CD4+ T cells regulate IL-22 producing intestinal innate lymphoid cells. Mucosal immunology, 7(5), 1045-1057.
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5

Investigating Immune Responses in Helminth Infection

Cited 1 time
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Third-stage larvae (L3) of N. brasiliensis were purified with a Baermann apparatus. After washing three times in PBS, living worms were counted. On day 0, 500 purified worms were injected subcutaneously in PBS. In addition, in some experiments an anti-CD4 depleting antibody (clone GK1.5, 250 μg/mouse, in house) was injected intraperitoneally (i.p.) at day 0 and day 2, 4, and 6 of N. brasiliensis infection. In some experiments, anti-ICOS antibody (500 μg/mouse, Bioxcell) and rat IgG isotype control (500 μg/mouse, Sigma-Aldrich) were injected i.p. at day 1 and day 3 of N. brasiliensis infection and analyzed on day 5 post-infection, or treated with anti-ICOS antibody or rat IgG isotype control every other day for six days and analyzed one day later. In some experiments, mice were treated with carrier-free recombinant murine IL-33 (1 μg/mouse, R&D Systems) intravenously (i.v.) and analyzed 24 h later or treated with IL-33 (500 ng/mouse) or IL-25 (250μg/mouse, R&D Systems) i.p. for three consecutive days and analyzed one day later. In the IL-33 rescue experiments, mice were injected i.p. with IL-33 (12.5 μg/kg body weight/day) (Brestoff et al., 2015 (link)) from day 1 to day 6 of N. brasiliensis infection and analyzed 24 h later.
Flamar A.L., Klose C.S., Moeller J.B., Mahlakõiv T., Bessman N.J., Zhang W., Moriyama S., Stokic-Trtica V., Rankin L.C., Putzel G.G., Rodewald H.R., He Z., Chen L., Lira S.A., Karsenty G, & Artis D. (2020). Interleukin-33 induces the enzyme tryptophan hydroxylase 1 to promote inflammatory group 2 innate lymphoid cell-mediated immunity. Immunity, 52(4), 606-619.e6.
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