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6 well ultra low binding plates

Manufactured by Corning
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Sourced in China

The 6-well ultra-low binding plates are a laboratory equipment product designed to minimize non-specific binding of biomolecules. They feature a specialized surface treatment that reduces the adsorption of proteins, cells, and other biological samples, enabling more reliable and consistent experimental results.

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Lab products found in correlation

Humec media, by Thermo Fisher Scientific (1 mentions) 40 μm pore cell strainer, by BD (1 mentions) Eclipse ts100 microscope, by Nikon (1 mentions)

2 protocols using 6 well ultra low binding plates

1

Mammosphere Formation Assay Protocol

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The different groups of cells (0.5 × 104 cells/well) were cultured in triplicate in 6-well ultra-low binding plates (Corning) with DMEM-F12 medium (Heart, China), supplemented with 20 ng/ml EGF, 20 ng/ml basic FGF, 1× B27 for 14 days. Spheres over 100 mm diameter (MDA-MB-231) or 50 mm diameter (MCF-7) were counted and pictured using Nikon microscope.
Zhao L., Lei J., Gu S., Zhang Y., Jing X., Wang L., Zhang L., Ning Q., Luo M., Qi Y., Zhao X, & Shao S. (2022). A yes-associated protein 1- Notch1 receptor positive feedback loop promotes breast cancer lung metastasis by attenuating the bone morphogenetic protein 4-SMAD family member 1/5 signaling. Carcinogenesis, 43(12), 1162-1175.
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2

Oncosphere Assay for Cancer Cells

Cited 1 time
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Oncosphere assays were performed as previously described30 (link). Briefly, cancer cells were collected by using scraper and filtered using 40 μm pore cell strainer (BD Biosciences). 0.5–2.5 × 104 single cells were seeded into 6-well ultra-low binding plates (Corning) and cultured in Human mammary epithelial cell (HuMEC) media (Life Technologies) containing 20 ng ml−1 EGF, 5 μg ml−1 insulin, 10 μg ml−1 heparin, 40 ng ml−1 basic FGF, 1X B27 supplement without vitamin A and 0.02% methylcellulose for 7 days. Spheres over 100 μm diameter were counted and pictured using Nikon ECLIPSE TS100 microscope.
Song K.H., Park M.S., Nandu T.S., Gadad S., Kim S.C, & Kim M.Y. (2016). GALNT14 promotes lung-specific breast cancer metastasis by modulating self-renewal and interaction with the lung microenvironment. Nature Communications, 7, 13796.
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