Hydrogen peroxide

Manufactured by Thermo Fisher Scientific

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Hydrogen peroxide is a chemical compound with the formula H2O2. It is a clear, colorless liquid that is used as an oxidizing agent in various industrial and laboratory applications.

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Lab products found in correlation

229 protocols using hydrogen peroxide

Yeast Stress Tolerance Assay

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Yeast cells were adjusted in concentration to an initial A₆₀₀ of 0.5, and were then serially diluted 1:10 and spotted onto plates supplemented with MnCl₂ (Sigma, St. Louis, MO, USA; 244589), or hydrogen peroxide (Alfa Aesar, Heysham, UK; L14000) at the indicated concentrations. The plates were incubated at 26 °C over the course of 2–3 days.

de Oya I.G., Jiménez-Gutiérrez E., Gaillard H., Molina M., Martín H, & Wellinger R.E. (2022). Manganese Stress Tolerance Depends on Yap1 and Stress-Activated MAP Kinases. International Journal of Molecular Sciences, 23(24), 15706.

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Synthesis and Analysis of Glucose Derivatives

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3-mercaptophenylboronic acid (3-MPBA) (≥95%), 3-hydroxythiophenol (3-HTP) (≥96%), D-(+)-glucose (≥99.5% GC grade), D-(−)-fructose (≥99%), L-glutamine (≥99%), phorbol 12-myristate 13-acetate (PMA) (≥99%) and sodium hypochlorite (5% w/w) are purchased from Sigma-Aldrich. D-(+)-galactose (≥98%), D-(+)-mannose (≥99%), urea (≥99%), sodium bromide (≥99%), sodium nitroprusside (≥98%), hydrogen peroxide (35% w/w), tert-butyl hydroperoxide (TBHP) (70% w/w), calcium sulfate dehydrate (≥98%) and sodium dihydrogen phosphate (≥98%) are purchased from Alfa Aesar. D-(+)-sucrose (≥99%), creatinine (≥99%) is purchased from TCI. Glucose oxidase from aspergillus and sodium chloride (≥99%) are purchased from VWR. Iron (II) chloride tetrahydrate (≥99%) is purchased from JT Baker. Magnesium sulfate (≥98%) is purchased from EMD Millipore. Potassium chloride (≥99%) is purchased from Acros Organics. Ultrapure water is obtained from a Millipore water system. Citrate-capped gold nanoparticles (60 nm, 5.2×10⁻¹¹ M) are purchased from Nanopartz. Normal human serum is purchased from UTAK Laboratories. Artificial urine is made from 55 mM sodium chloride, 67 mM Potassium chloride, 2.6 mM calcium sulfate, 29.6 mM sodium sulfate, 3.2 mM Magnesium sulfate, 19.8 mM sodium dihydrogen phosphate, 9.8 mM creatinine and 310 mM urea.

Gu X., Wang H., Schultz Z.D, & Camden J.P. (2016). Sensing Glucose in Urine & Serum and Hydrogen Peroxide in Living Cells Using a Novel Boronate Nanoprobe Based on Surface-Enhanced Raman Spectroscopy. Analytical chemistry, 88(14), 7191-7197.

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Enzymatic Assays for Catalytic Activity

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Horseradish peroxidase (HRP) (reagent grade), Candida antarctica lipase (CALB), 2-methylimidazole, phosphate buffer saline (1×), 1,2,3-trihydroxybenzene (THB, ACS reagent), and 4-nitrophenyl butyrate (p-NPB) were purchased from Sigma-Aldrich. Zinc nitrate hexahydrate (99.998%) and hydrogen peroxide (29–32% wt) were purchased from Alfa Aesar. Cytochrome c from horse heart was purchased from Biodee Corporation. All the other reagents are of analytic grade.

Wu X., Yang C, & Ge J. (2017). Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents. Bioresources and Bioprocessing, 4(1), 24.

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Synthesis and Antibacterial Assessment of Zinc Oxide Nanoparticles

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Standard LEVO was obtained from Sanofi pharmaceutical company (Egypt). For the preparation of NPs: zinc acetate dihydrate, sodium hydroxide, sulfuric acid (95%), sodium nitrate (99.9%), and potassium permanganate (99.9%, KMnO₄) were purchased from sigma Aldrich; Graphite (99.99%) and hydrogen peroxide (H₂O₂ 36%) from Alfa Aesar; Methanol HPLC grade (99.8%) from LobaChemie PVT.LTD, India; and Potassium dihydrogen phosphate and ortho-phosphoric acid from Sharlau, Spain. For the antibacterial activity test: saline solution, barium chloride, sulphuric acid (ADWIC company, Egypt) and Mueller-Hinton agar were used.

El-Maraghy C.M., El-Borady O.M, & El-Naem O.A. (2020). Effective Removal of Levofloxacin from Pharmaceutical Wastewater Using Synthesized Zinc Oxid, Graphen Oxid Nanoparticles Compared with their Combination. Scientific Reports, 10, 5914.

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Extraction and Characterization of Polyphenols

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Flake graphite, sodium nitrate (NaNO₃), hydrochloric acid (HCl, 37%), sulfuric acid (H₂SO₄, 98%), potassium permanganate (KMnO₄, 99.9%), hydrogen peroxide (H₂O₂, 30%), sodium carbonate (NaCO₃) and ammonia were procured from Alfa Aesar. All drugs are analytically pure. Beer (Budweiser Beer Co. Ltd, China), wine (Yantai Changyu Grape Brewing Co. Ltd, China) and cocoa (No Sikkim Cocoa Food Co. Ltd, China) are bought from a nearby supermarket and simply purified before be used. The content of tannic acid was determined by tannic acid analysis test method based on Chinese forestry standard LY/T 1642-2005. The content of tannic acid in beer, wine and cocoa was 13.35%, 19.92% and 8.75%, respectively. The water used throughout the experiments was DI-water.

Xu J., Lu L., Duan G, & Zhao W. (2022). Characteristic analysis of three-ecofriendly reduced graphene oxides (rGOs) and their application in water–ethanol-based fluids with different volume ratios. RSC Advances, 12(9), 5522-5533.

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Destabilized ATP Aptamer Purification

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The “destabilized” ATP aptamer (5′- CTGGGGGAGTATTGCGGAGGAAA-3′) oligonucleotide sequence^{24 (link)} was purified using dual HPLC (Biosearch Technologies, CA) and used as received. Tris-2-carboxyethyl-phosphine (TCEP), ferrocenemethanol (FcMeOH), 6-mercapto-1-hexanol (99%), Trizma (tris) base (2-amino- 2-(hydroxymethyl)-1,3-propanediol, magnesium chloride (MgCl₂), sulfuric acid (H₂SO₄), potassium chloride (KCl), hydrogen peroxide (H₂O₂), hydrochloric acid (HCl) sodium chloride (NaCl), adenosine triphosphate (ATP), dopamine hydrochloride (99%) (Alfa Aesar), dimethyl sulfoxide (DMSO), and 10× Tris-EDTA were used as received from Sigma- Aldrich. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25°C) using a Biopak Polisher Millipore ultra purification system (Millipore, Billerica, MA).

Santos-Cancel M., Simpson L.W., Leach J.B, & White R.J. (2019). Direct, Real-Time Detection of Adenosine Triphosphate Release from Astrocytes in 3-Dimensional Culture Using an Integrated Electrochemical, Aptamer-Based Sensor. ACS chemical neuroscience, 10(4), 2070-2079.

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Colorimetric Detection of Biomolecules

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Chlorauric acid (HAuCl₄∙3H₂O), potassium tetrachloroplatinate (II) (K₂PtCl₄), cetyltrimethylammonium bromide (CTAB), hydrogen peroxide, L-ascorbic acid (AA), 3,3′,5,5′-tetramethylbenzidine (TMB), cysteine, L-glutathione reduced (GSH), L-glutathione oxidized (GSSG), horseradish peroxidase and sodium hydrosulfide (NaHS) were purchased from Alfa Aesar. FeSO₄∙6H₂O, Co(NO₃)₂∙6H₂O, ZnSO₄, CuSO₄, Mg₂SO₄, Na₂S∙9H₂O, glucose, uric acid and glycine were obtained from Sinopharm Chemical Reagent Co., Ltd. Milli-Q water (18 MΩ cm) was used in the preparation of all solutions.

He W., Han X., Jia H., Cai J., Zhou Y, & Zheng Z. (2017). AuPt Alloy Nanostructures with Tunable Composition and Enzyme-like Activities for Colorimetric Detection of Bisulfide. Scientific Reports, 7, 40103.

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Colorimetric MoSe2 Nanoparticle Synthesis

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MoSe₂ powder (99.9%), 3,3,5,5-tetramethylbenzidine (TMB), and isopropanol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cetyltrimethyl ammonium bromide (CTAB) was purchased from Millipore (Burlington, MA, USA). Acetic acid, sodium acetate anhydrous, hydrochloric acid, and hydrogen peroxide (35%) were obtained from Alfa Aesar (Tewksbury, MA, USA). These chemicals were of analytical purity and were used as received. Deionized water (DI water) was used as a solvent throughout.

Hang D.R., Pan Y.Q., Sharma K.H., Chou M.M., Islam S.E., Wu H.F, & Liang C.T. (2020). 2D CTAB-MoSe2 Nanosheets and 0D MoSe2 Quantum Dots: Facile Top-Down Preparations and Their Peroxidase-Like Catalytic Activity for Colorimetric Detection of Hydrogen Peroxide. Nanomaterials, 10(10), 2045.

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Zirconium-based Metal-Organic Framework Synthesis

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Zirconium(IV) chloride
(ZrCl4, 98%, Acros), 2-aminoterephthalic acid (99%, Acros), hydrochloric
acid (HCl, 36%, Honeywell), N,N-dimethylformamide
(DMF, 99.5%, Fisher), ethanol (C₂H₅OH, 99.9%,
Honeywell), acetonitrile (99.9%, Honeywell), n-dodecane
(99%, Alfa Aesar), hydrogen peroxide (H₂O₂,
30%, Alfa Aesar), and dibenzothiophene (98%, Acros) were used as received.

Barghi B., Jürisoo M., Volokhova M., Seinberg L., Reile I., Mikli V, & Niidu A. (2022). Process Optimization for Catalytic Oxidation of Dibenzothiophene over UiO-66-NH2 by Using a Response Surface Methodology. ACS Omega, 7(19), 16288-16297.

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Synthesis of Titanium-based Nanomaterials

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Analytical grade chemicals were used in this study without any purification. Tetrabutyl titanate ((TBT), Sigma Aldrich), titanium tetrachloride (Alfa Aesar), toluene (99.9%, Sigma Aldrich), ethylene glycol (VWR Chemicals), ethanol, iron(III) chloride hexahydrate (Sigma Aldrich), cobalt chloride hexahydrate (Acros Organics), polyethylene glycol (PEG-8000, Alfa Aesar), sodium acetate (Alfa Aesar), graphite powder (Fisher Chemicals), sulphuric acid (98%, VWR Chemicals), sodium nitrate (Sigma Aldrich), potassium permanganate (Sigma Aldrich), hydrogen peroxide (30%, Alfa Aesar), cobalt oxide (50–80 nm diameter, Alfa Aesar), potassium dichromate (Sigma Aldrich), bisphenol A (Sigma Aldrich) were used in this study.

Santhosh C., Malathi A., Daneshvar E., Kollu P, & Bhatnagar A. (2018). Photocatalytic degradation of toxic aquatic pollutants by novel magnetic 3D-TiO2@HPGA nanocomposite. Scientific Reports, 8, 15531.

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