Streptozotocin (100 mg) was freshly dissolved in 1 ml of 0.1 M citrate buffer (pH 4.5) and was administered to various groups, based on the body weight of the rats and at a dosage of 45 mg/kg body weight. The administration was done subcutaneously in vehicle volume of 0.07 ml using a syringe with fine needle [42 (link)]. A rest period of two days (48 hours) following induction of diabetes was allowed for the blood glucose level to stabilize. During this period, the animals had free access to both water and food. Blood glucose was measured by a glucometer (One Touch Ultra®, LifeScan, USA), and only rats with blood glucose > 200 mg/dl were considered hyperglycemic. The hyperglycemic rats (n = 30) were divided into 6 groups comprising 5 rats in each group for the study.
Onetouch ultra
Manufactured by LifeScan
Sourced in United States, France, United Kingdom, Canada
The OneTouch Ultra is a blood glucose monitoring device designed to measure the level of glucose in a user's blood. It provides accurate and reliable results to help individuals manage their diabetes.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (6 mentions)
Labassay triglyceride, by Fujifilm (5 mentions)
Insulin, by Eli Lilly (5 mentions)
Labassay cholesterol, by Fujifilm (4 mentions)
Humulin r, by Eli Lilly (4 mentions)
Labassay nefa, by Fujifilm (4 mentions)
Ultra sensitive mouse insulin elisa kit, by Crystal Chem (4 mentions)
Human insulin, by Merck Group (3 mentions)
Dpp4 inhibitor, by Merck Group (3 mentions)
D glucose, by Merck Group (3 mentions)
Triglyceride determination kit, by Merck Group (3 mentions)
Eia kit, by ALPCO (2 mentions)
D12492, by Research Diets (2 mentions)
High fat diet (hfd), by Research Diets (2 mentions)
Model 7170, by Hitachi (2 mentions)
Cgms ipro, by Medtronic (2 mentions)
Mouse rat pyy elisa kit, by Fujifilm (1 mentions)
Glp 1 active elisa kit, by Merck Group (1 mentions)
Accu check performa, by Roche (1 mentions)
Automatic analyzer model 7170, by Hitachi (1 mentions)
231 protocols using onetouch ultra
1
Streptozotocin-Induced Diabetes in Rats
2
NKT Cells and OVA-specific CD8 T Cells
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A total of 2 × 106 NKTGFP and NKTSV cells were pre-injected into RIP-OVA mice, which were subsequently injected peritoneally with 8 × 106 OVA-specific CD8 T cells and 2 × 105 GFP-DCs loaded with OT-I peptides. The blood glucose was monitored using a One-Touch Ultra glucometer (LifeScan, CA).
3
Glucose and Hormone Measurements in Mice
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Blood glucose concentrations were measured using a One Touch Ultra (LifeScan, Milpitas, CA, USA). The concentrations of plasma and fecal triglycerides (LabAssay Triglyceride; Wako Pure Chemical Co. Ltd., Osaka, Japan), total cholesterol (LabAssay Cholesterol; Wako Pure Chemical Co. Ltd.), PYY (Mouse/Rat PYY ELISA Kit, Wako), GLP-1 (active) ELISA kit (Merck Millipore, Billerica, MA, USA), and an insulin ELISA Kit (Shibayagi, Gunma, Japan) were measured according to manufacturer instructions. For GLP-1 measurement, plasma samples and culture media were treated with a dipeptidyl peptidase IV (DPP-IV) inhibitor (Merck Millipore) to prevent degradation of active GLP-1.
For GTT, 24-h-fasted obese mice were given 1.5 mg of glucose/gram of body weight intraperitoneally (i.p.). For ITT, 3-h-fasted obese mice were administered human insulin (0.75 mU/g by i.p.; Sigma-Aldrich, St. Louis, MO, USA). Plasma glucose concentration was monitored before injection and at 15-, 30-, 60-, and 120-min post-injection.
For GTT, 24-h-fasted obese mice were given 1.5 mg of glucose/gram of body weight intraperitoneally (i.p.). For ITT, 3-h-fasted obese mice were administered human insulin (0.75 mU/g by i.p.; Sigma-Aldrich, St. Louis, MO, USA). Plasma glucose concentration was monitored before injection and at 15-, 30-, 60-, and 120-min post-injection.
4
Fasting Blood Glucose and Lipid Assays
5
Insulin Sensitivity Assessment in Mice
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Animals were weighed on a weekly basis systematically at 9:00-10:00 during the entire protocol. They were five-hour fasted prior blood glucose determination (One Touch Ultra, LifeScan Inc., Wayne, PA, United States). Additionally, to assess insulin sensitivity, all mice underwent an insulin tolerance test (ITT) by the end of the 18th week. Mice were short-fasted for 5 h, basal blood glucose was determined and shortly after this, 100 μl saline solution containing a standardized dose of 0.025 IU of human-recombinant short-acting insulin (Humulin R, Lilly, Indianapolis, IN, United States) was intraperitoneally administered in every animal. Blood glucose measurement was repeated thereafter at 30 min and 60 min. An additional solution of dextrose in sterile water was ready to use in case an animal might be at risk of death by the hypoglycemic effect of short-acting insulin. Once the protocol was finished, all animals were given free access to food and water. No animal losses occurred during the ITT.
6
Glucose and Insulin Tolerance Tests
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Glucose tolerance test (GTT) was performed by administrating glucose (2.0 mg/g BW) intraperitoneally after a 16-hour fast. Blood glucose levels were monitored using glucose test strips and a glucometer (OneTouch ultra, LifeScan) at indicated times. Blood was also collected from tails using EDTA-treated microcapillaries and plasma insulin levels were measured using an EIA kit (ALPCO). For insulin tolerance test (ITT), mice were fasted for 4 hours and injected insulin (1.0 mU/g BW, Lilly) intraperitoneally, and blood glucose levels were measured at indicated times.
7
Insulin-Induced Glucose Monitoring in Mice
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Following an overnight fast, mice received an injection of insulin (0.75 units/kg body wt i.p.; Humulin R; Eli Lilly, Indianapolis, IN). Blood glucose was monitored (OneTouch Ultra; LifeScan) before and at serial time points after insulin administration.
8
Glucose Tolerance Test in Mice
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After 16 h of fasting, mice were injected intraperitoneally with glucose (2 g/kg). Blood glucose levels were measured from the tail vein at indicated times using a glucometer (One Touch Ultra; LifeScan Inc.).
9
Fasting and Postprandial Metabolic Profiling
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After the completion of anthropometric measurement, rats were sacrificed under anesthesia using 3% isoflurane in either fasting (19:00) or post-prandial (7:00) state, 6 rats in each time point. Blood samples were collected via abdominal aorta, and treated immediately as described below. Liver, brown adipose tissue (BAT) and skeletal muscle were obtained and cut into small pieces and immediately frozen in liquid nitrogen, then stored at −80°C.
Blood glucose concentrations were measured with a handheld glucose meter (One Touch Ultra; LifeScan, Milpitas, CA) immediately after blood sampling. Blood lactate and pyruvate were determined enzymatically with spectrophotometric assays. The remaining blood samples were centrifuged (3000rpm, 10min), and serum samples were separated and stored at −80°C until the assay. Serum concentrations of insulin were determined with a Rat Insulin ELISA KIT (Shibayagi, Gunma, Japan).
Blood glucose concentrations were measured with a handheld glucose meter (One Touch Ultra; LifeScan, Milpitas, CA) immediately after blood sampling. Blood lactate and pyruvate were determined enzymatically with spectrophotometric assays. The remaining blood samples were centrifuged (3000rpm, 10min), and serum samples were separated and stored at −80°C until the assay. Serum concentrations of insulin were determined with a Rat Insulin ELISA KIT (Shibayagi, Gunma, Japan).
10
Monitoring Diabetes in Mice
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Blood samples were obtained from the tail vein. Blood glucose was monitored with a OneTouch Ultra (LifeScan, Neckargemünd, Germany) twice weekly in unfasted mice. Animals with blood glucose values over 300 mg/dL on two consecutive days were considered diabetic.
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