Kribb11

Manufactured by MedChemExpress

Sourced in United States

KRIBB11 is a chemical compound that functions as a selective inhibitor of the AMPK-related kinase SNARK (also known as NUAK2). KRIBB11 inhibits the catalytic activity of SNARK in a dose-dependent manner. This compound can be used in research applications to study the biological functions of the SNARK kinase.

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Lab products found in correlation

6 protocols using kribb11

Microglia Activation Modulation Protocol

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LPS (Escherichia coli, serotype 0111: B4, #L2630) and poly-L-lysine (#25988-63-0) were purchased from Sigma (Saint Louis, MO, United States) and Santa Cruz Biotechonology (Santa Cruz, CA, United States), respectively. Both Hoechst 33258 (#HY-15558) and KRIBB11 (#HY-100872) are the products of MedChem Express (Princeton, NJ, United States). Antibodies against protein kinase B (Akt, #4691), phospho-Akt (#4060), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #5174) are the products of Cell Signaling Technology (Beverly, MA, United States). The antibody against Iba-1 (#ab178846) was purchased from Abcam (Cambridge, MA, United States). Both LY294002 (#440202) and VIII (#124018) were purchased from Calbiochem (San Diego, CA, United States). Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12) was obtained from Biotium and Gibco Invitrogen Corporation. Other agents were purchased from commercial suppliers. Stocked drugs solutions were stored at −20°C and diluted to the final concentration immediately before use. The final concentration of dimethyl sulfoxide (DMSO) is <0.05%. No toxic effect of DMSO was observed.

Su J., Dou Z., Hong H., Xu F., Lu X., Lu Q., Ye T, & Huang C. (2022). KRIBB11: A Promising Drug that Promotes Microglial Process Elongation and Suppresses Neuroinflammation. Frontiers in Pharmacology, 13, 857081.

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Endometriosis Cell Line Culture and Antibody Dilutions

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The endometriotic epithelial cell line (11Z) was established by Professor Anna Strazinski-Powitz [24 (link)]. The human endometrial stromal cell line (ESC) was established by Dr. Krikun [25 (link)]. All cell lines were cultured in Dulbecco’s Modified Eagle Medium/Ham’s F-12 50/50 Mix (DMEM/F-12) supplemented with 10% FBS (Gibco, Carlsbad, CA, USA) with 100 μg/mL penicilin and 100 μg/mL streptomycin at 37 °C and 5% CO₂.
Mouse anti-β-actin (A1978) was from Sigma-Aldrich, and dilution: 1:5000. Mouse anti-HSF1 (sc-17,757) was from Santa Cruz, and dilution: 1:1000. Rabbit anti-PFKFB3 (ab181861) was purchase from Abcam, and dilution: 1:2000. KRIBB11 were obtained from Med Chem Express (MCE), 50 mg/kg.

Wang Y., Xiu J., Yang T., Ren C, & Yu Z. (2021). HSF1 promotes endometriosis development and glycolysis by up-regulating PFKFB3 expression. Reproductive Biology and Endocrinology : RB&E, 19, 86.

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Compound Dissolution in Diverse Solvents

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Sodium arsenite (S7400), chloroquine diphosphate salt (C6628), and ammonium chloride (A9434) purchased from Sigma were dissolved in ddH₂O. actinomycin D (A1410), MG132 (474787) and geldanamycin (G3381) purchased from Sigma were dissolved in dimethyl sulfoxid (DMSO). HSF1 inhibitor KRIBB11 purchased from Medchemexpress (HY-100872) was dissolved in DMSO.

Lin Y.L., Tsai H.C., Liu P.Y., Benneyworth M, & Wei L.N. (2017). Receptor-interacting protein 140 as a co-repressor of Heat Shock Factor 1 regulates neuronal stress response. Cell Death & Disease, 8(12), 3203.

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Cerulein-Induced Pancreatic Injury Model

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Cerulein (40 μg/kg or 5 μg/mouse), KRIBB11 (mainly 2 μM for cells and 50 mg/kg for mice), erlotinib (2 μM for cells and 100 mg/kg for mice), selumetinib (100 nM for cells) and torkinib (50 nM for cells) were purchased from MedChem Express (Monmouth Junction, NJ, USA). Recombinant human epidermal growth factor (EGF mainly 20 ng/ml for cells) and transforming growth factor alpha (mainly TGFα 50 ng/ml for cells) were purchased from PeproTech (Rocky Hill, NJ, USA). All reagents were stored according to the manufacturer’s instructions.

Qian W., Chen K., Qin T., Xiao Y., Li J., Yue Y., Zhou C., Ma J., Duan W., Lei J., Han L., Li L., Shen X., Wu Z., Ma Q, & Wang Z. (2021). The EGFR-HSF1 axis accelerates the tumorigenesis of pancreatic cancer. Journal of Experimental & Clinical Cancer Research : CR, 40, 25.

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Establishing 4T1 Murine Mammary Carcinoma Model

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For both in vitro and in vivo studies, the 4T1 murine mammary carcinoma cell line, provided by Judy Lieberman (Lieberman Laboratory, Harvard University, Boston, MA, USA), was used. The 4T1 cells were grown as adherent culture in Dulbecco’s Modified Eagle Medium (DMEM high glucose, 4.5 g/L without lglutamine and Phenol Red, Capricorn Scientific, Ebsdorfergrund, Germany, Cat-No. DMEM-HXRXA) supplemented with 10% Fetal Bovine Serum (FBS—South America Origen, EU approved, EuroClone S.p.A., Pero, Italy, CatNo. ECS0180L), l-glutamine 200 mM (Capricorn Scientific, Ebsdorfergrund, Germany, Cat-No. GLN-B), and penicillin/streptomycin 100x (Capricorn Scientific, Ebsdorfergrund, Germany, Cat-No. PS-B). Cells were submitted to passages every 2 or 3 days. Trypsin 10x (Lonza A. G., Basel, Switzerland, Cat-No. 17-160E) was used to release cells from sub-confluent monolayers. The detached cells were seeded back into cell culture flasks or prepared for experiments. KRIBB11 was purchased from MedChemExpress (Monmouth Junction, NJ, USA, Cat-No. HY-100872).

Viana P.H., Schvarcz C.A., Danics L.O., Besztercei B., Aloss K., Bokhari S.M., Giunashvili N., Bócsi D., Koós Z., Benyó Z, & Hamar P. (2024). Heat shock factor 1 inhibition enhances the effects of modulated electro hyperthermia in a triple negative breast cancer mouse model. Scientific Reports, 14, 8241.

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Cerulein, KRIBB11, and Erlotinib Protocol

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Cerulein (40 µg/kg or 5 µg/mouse), KRIBB11 (1,2 5, 10, 20 µM for cells and 50 mg/kg for mice), erlotinib (10 µM for cells and 100 mg/kg for mice) were purchased from MedChem Express (Monmouth Junction, NJ, USA). Recombinant human epidermal growth factor (EGF, 20 ng/ml for cells) was purchased from PeproTech (Rocky Hill, NJ, USA). All reagents were stored according to the manufacturer's instructions.

Qian W., Chen K., Qin T., Xiao Y., Li J., Yue Y., Zhou C., Ma J., Duan W., Lei J., Liang H., Shen X., Wu Z., Ma Q., & Wang Z. (2020). The EGFR-HSF1 Axis Accelerates the Tumorigenesis of Pancreatic Cancer.

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