NOD-scid IL2Rgammanull (NSG) mice, used at 8–12-weeks-old, were purchased from The Jackson Laboratory or bred in-house. RH30_19 tumor cells were prepared at 20×106/mL in PBS. 50 μL (1×106 RH30_19 or RH30_19 MIF KO) of the single cell suspension were injected intramuscularly into the right hind leg of NSG mice. T cells (CAR T or UTD) were injected intravenously or intratumorally, as per procedure. Mice were imaged weekly after tumor inoculation using IVIS Spectrum in vivo Imaging system (PerkinElmer) 15 minutes after subcutaneous D-luciferase (Xenolight D-luciferin K+ salt, PerkinElmer) injection. Endpoint was determined by tumor volume (1,000 mm3) as measured by digital caliper using the formula: volume = (width2 * length)/2. All-trans retinoic acid (ATRA) was delivered by timed release subcutaneous pellet (5 mg, 21-day release, Innovative Research of America). PLX3397 (50 mg/kg/d, MedChemExpress), Epacadostat (75 mg/kg/d, MedChemExpress; refs. 30, 31 (link)), and SD-208 (25 mg/kg/d, MedChemExpress; ref. 32 (link)) were delivered by oral gavage using plastic feeding tubes (Instech, FTP-18–30). L-NAME (50 mg/kg/d, Cayman Chemical) and anti-PD1 antibody (200 μg/dose, BioXCell, BE0188) were delivered by intraperitoneal injection. Doses were 5 days on/2 days off unless otherwise noted.
Ftp 18 30
Manufactured by Instech
Sourced in United Kingdom
The FTP-18–30 is a laboratory equipment that functions as a flue gas analyzer. It is designed to measure the composition of exhaust gases, including the levels of oxygen, carbon monoxide, and other gaseous components.
Automatically generated - may contain errors
Lab products found in correlation
Be0188, by BioXCell (2 mentions)
Xenolight d luciferin k salt, by PerkinElmer (2 mentions)
Ivis spectrum in vivo imaging system, by PerkinElmer (2 mentions)
Epacadostat, by MedChemExpress (2 mentions)
Nod scid il2rgammanull nsg mice, by Jackson ImmunoResearch (1 mentions)
L name, by Cayman Chemical (1 mentions)
3 protocols using ftp 18 30
1
Tumor Growth and Immunotherapy in NSG Mice
2
Murine Xenograft Model for Rhabdomyosarcoma
3
Murine Bronchoalveolar Lavage Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
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After cutting open the abdominal cavity, the diaphragm was carefully punctured. The skin and tissue were removed from the throat until the trachea was revealed. A cut was inserted into the trachea using a sharp blade and an 18-gauge polypropylene gavage tube (with the tip removed: FTP-18–30: , Instech, Leicestershire, UK) inserted into the incision. The mouse lungs were ravaged to inflate the lung three times with 1 ml of PBS and transferred to a 1.5 ml Eppendorf tube. The BAL fluid was centrifuged at 1500 rpm for 5 minutes and supernatants were collected and frozen at -80°C. The cell pellet was resuspended with 500 µl of Ammonium–Chloride–Potassium (ACK) lysis buffer for 2 minutes then quenched with PBS prior to centrifugation at 1500 rpm for 5 minutes prior to resuspending in RPMI for flow cytometry.
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