Twenty seven established cutaneous melanoma cell lines were used in this study. Twenty three early-passage (<20) melanoma lines were established from AJCC stage III and IV melanoma patients who received elective surgery at JWCI and were authenticated with autologous peripheral blood leukocytes. Stage I/II melanoma lines (WC00060, WC00080, WC00081 and WC00062) were obtained from Coriell Institute (Camden, NJ) and have been tested by short tandem repeat DNA profiles. Cells were cultured in a humidified chamber with 5% CO2 at 37°C in either RPMI-1640 (Corning, Corning, NY) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gemini Bio-Products, Sacramento, CA) and 1% penicillin-streptomycin, or MCDB-153 (Sigma-Aldrich, St. Louis, MO) / Leibovitz’s L-15 (Life Technologies, Carlsbad, CA) supplemented with 2% FBS, Insulin (5 μg/ml, Sigma-Aldrich), and 1% penicillin-streptomycin (9 (link),17 (link)). All the cell line experiments were completed within 12 passages or <4 months from thawing.
Wc00080
Manufactured by Coriell Institute for Medical Research
The WC00080 is a laboratory centrifuge designed for general-purpose applications. It is a compact and versatile instrument capable of separating samples at high speeds. The centrifuge can accommodate a variety of sample tubes and microplate formats. Its key function is to facilitate the separation of components within liquid samples through the application of centrifugal force.
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Lab products found in correlation
Wc00060, by Coriell Institute for Medical Research (2 mentions)
Wc00081, by Coriell Institute for Medical Research (2 mentions)
Wc00062, by Coriell Institute for Medical Research (1 mentions)
Mcdb153, by Merck Group (1 mentions)
Leibovitz s l 15, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Rpmi 1640, by Corning (1 mentions)
Wm266 4, by American Type Culture Collection (1 mentions)
5 aza 2 deoxycytidine, by Merck Group (1 mentions)
2 protocols using wc00080
1
Melanoma Cell Line Characterization
2
Evaluating RASSF8 Expression in Melanoma
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We assessed expression of RASSF8 in 24 well-established, early-passaged cutaneous melanoma metastasis cell lines: M24, M101, FD0836, TG0873, M14, JH1173, ME7, M16, M20, CS0169, WP0614, ME35, BD0548, SR0488, AB0801, DP0574, M8133, LM0615, ME16, M21, ME9, M12, M211, and M13 established from AJCC stage III and IV melanoma patients who received surgery at JWCI.
Melanocyte line (HEM-M, Catalog #2216) was purchased from ScienCell (Carlsbad, CA), Wm266-4 from ATCC (Manassas, VA), and primary cell lines (WC00060, WC00080, WC00081) obtained from Coriell institute (Camden, NJ). Melanoma cell lines were maintained in appropriate culture medium supplemented with 10% fetal bovine serum (heat inactivated) and antibiotics at 37°C in a 5% CO2 atmosphere incubator, as previously described, and were used at early passages [26 (link), 27 (link)]. Primary cells and melanocyte cell lines were cultured following manufacturer's instructions. For methylation assay studies, cultured cells were treated with 2 μmol/L 5-aza-2-deoxycytidine (Sigma-Aldrich, St.Louis, MO) dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St.Louis, MO) for 72 hrs with media changed every 24 hrs as previously described [10 (link)]. Control cells were incubated with DMSO under the same culture conditions.
Melanocyte line (HEM-M, Catalog #2216) was purchased from ScienCell (Carlsbad, CA), Wm266-4 from ATCC (Manassas, VA), and primary cell lines (WC00060, WC00080, WC00081) obtained from Coriell institute (Camden, NJ). Melanoma cell lines were maintained in appropriate culture medium supplemented with 10% fetal bovine serum (heat inactivated) and antibiotics at 37°C in a 5% CO2 atmosphere incubator, as previously described, and were used at early passages [26 (link), 27 (link)]. Primary cells and melanocyte cell lines were cultured following manufacturer's instructions. For methylation assay studies, cultured cells were treated with 2 μmol/L 5-aza-2-deoxycytidine (Sigma-Aldrich, St.Louis, MO) dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St.Louis, MO) for 72 hrs with media changed every 24 hrs as previously described [10 (link)]. Control cells were incubated with DMSO under the same culture conditions.
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