Poly allylamine hydrochloride

Melanoma cell lines for this study were obtained from The European Searchable Tumour Line Database (ESTDAB: http://www.ebi.ac.uk/ipd/estdab) and were maintained in RPMI-1640 medium containing 10% FBS and antibiotics at 37 °C and 5% CO₂. The PCR primers for this study have been modified from Dahl et al.^{27 (link),28 (link)} and were obtained from DNA Technology A/S, Denmark. The sequences can be found in the Supporting Information Table S2. The amine modified DNA probes (sequences in Supporting Information Table S1) were matched for melting temperature calculated with nearest-neighbor model.^{33 (link)} The probes were obtained from DNA Technology A/S. The other reagents: poly(ethylene-alt-maleic anhydride) (Sigma-Aldrich), poly(allylamine hydrochloride) (Polyscience), distilled water (Invitrogen), 1-Ethyl-3-(3-(dimethylamino)propyl)-carbodiimide EDC (Sigma-Aldrich), N-hy-droxysuccinimide NHS (Sigma-Aldrich) 1% bovine serum albumin BSA (Sigma-Aldrich), phosphate buffered saline PBS (Gibco), distilled water (Invitrogen), Tween 20 (Sigma-Aldrich), Urea (Fisher Scientific), 20× saline sodium citrate SSC (Invitrogen), mineral oil (Sigma-Aldrich), MNPs Streptavidin MicroBeads (Miltenyi), magnetic separation columns μ Columns (Miltenyi).