Mouse recombinant ifn β

HEK293, L929, and RAW264.7 were obtained from American Type Culture Collection. Huh7 cells were gift from Dr. Xiaoben Pan (Peking University Hepatology Institute). IFNαR and STAT1-deficient immortal BMDMs were gift from Dr. Genhong Cheng (University of California, Los Angeles). Mouse primary PMs were prepared from female C57BL/6J mice (5–6-week old) through intraperitoneal injection with thioglycolate. The cells were cultured at 37 °C under 5% CO₂ in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Biology Industries), 100 U ml⁻¹ penicillin (Beyotime) and 100 µg ml⁻¹ streptomycin (Beyotime). Primary bone marrow cells were collected from WT and NDR1^−/− mice to prepare bonemarrow-derived macrophages, which were cultured at 37 °C under 5% CO₂ in RPMI-1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Biology Industries) FBS, 20 ng ml⁻¹ M-CSF (PeproTech), 100 U ml⁻¹ penicillin (Beyotime) and 100 µg ml⁻¹ streptomycin (Beyotime). Mouse recombinant IFNβ, IFNγ and human recombinant IFNα were purchased from PeproTech. Poly (I:C), poly (dA:dT) and anti-flag mouse magnetic (M2) beads were from Sigma-Aldrich. Information about the antibodies used in this study is provided in Supplementary Table 1.