PvLDH levels in VIS were measured in whole blood samples collected between day 4 and day of treatment, and every 12 hours after treatment until 120 hours. PvLDH levels in the samples above were measured using the Q-Plex™ Human Malaria assay (Quansys Biosciences, Logan, UT, USA) as previously described27 (link). The Q-View™ Imager Pro was used to capture chemiluminescent images of each plate which was then quantitatively analysed using Q-View™ software (Quansys Biosciences).
Q view software
Manufactured by Quansys Biosciences
Sourced in United States
Q-View Software is a data analysis tool developed by Quansys Biosciences. It provides users with the ability to analyze and interpret data generated from Quansys Biosciences' lab equipment and assays.
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Lab products found in correlation
Q plex array human angiogenesis antigen, by Quansys Biosciences (3 mentions)
Q plex human malaria assay, by Quansys Biosciences (2 mentions)
Q view imager pro, by Quansys Biosciences (2 mentions)
Q view imager ls, by Quansys Biosciences (2 mentions)
Multiplex elisa array, by Quansys Biosciences (2 mentions)
Chemidoctm xrs system, by Bio-Rad (2 mentions)
Nanodrop, by Thermo Fisher Scientific (2 mentions)
Q plex mouse cytokine screen 16 plex, by Quansys Biosciences (1 mentions)
Myecl imager, by Thermo Fisher Scientific (1 mentions)
0.22 μm pore filter, by Merck Group (1 mentions)
Q plex assay kit, by Quansys Biosciences (1 mentions)
Protease phosphatase inhibitor cocktail, by Cell Signaling Technology (1 mentions)
Total protein assay, by Bio-Rad (1 mentions)
Chemidoc, by Bio-Rad (1 mentions)
Q plex, by Quansys Biosciences (1 mentions)
Q plex mouse cytokine screen, by Quansys Biosciences (1 mentions)
Rpmi 1640 medium, by Sartorius (1 mentions)
Griess reagent assay, by Promega (1 mentions)
21 protocols using q view software
1
Quantifying PvLDH Levels in Malaria
2
Plasma Biomarker Profiling in Cancer
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Before starting the treatment, peripheral blood and bone marrow plasma (the initial 1 ml of bone marrow aspirate) samples were collected into EDTA-containing tubes. Both blood and bone marrow plasma samples from 124 of 511 patients enrolled in the study (24%) were available for analysis. Plasma was separated by centrifugation (2,000 rpm for 20 min at 4 °C) within 1 h from blood drawing and aliquoted into multiple cryovials. Plasma samples were stored at − 80 °C until use. Before analysis, plasma samples were thawed slowly in an ice bath and all analyses were done from a one-off thaw sample. CAFs were measured by using Q-Plex™ Array Human Angiogenesis Antigen (Quansys Biosciences, Logan, Utah) allowing the simultaneous quantification of the following factors: angiopoietin-2 (ANG-2), fibroblast growth factor-2 (FGF-2), hepatocyte growth factor (HGF), interleukin-8 (IL-8), platelet-derived growth factor-BB (PDGF-BB), tissue inhibitor of matrix metalloproteinase-1 and 2 (TIMP-1, TIMP-2), tumor necrosis factor-alpha (TNF-α), and vascular endothelial growth factor (VEGF), according to the manufacturer’s instructions. Secreted levels of CAFs were quantified through Q-View Software (Quansys Biosciences, Logan, Utah) in triplicate samples, and the mean results were used in biomarker analysis.
3
Phosphorylation Profiling of RTKs
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The basal phosphorylation status of 16 RTKs (EGFR, HER-2, HER-3, HER-4, HGF-R (c-MET), IGF-IR, INS-R, M-CSF R, MSP-R, PDGFRa, PDGFRb, SCF R, Tie-2, VEGFR1, VEGFR2, VEGFR3) was investigated in BxPc3 parental cells and its drug-resistant variants using the Proteome Profiler 96 Human Phospho-RTK Array 1 (Catalog # ARZ001) following the manufacturer's instructions (R&D Systems). The plate was imaged using a G-box imaging system (Invitrogen, UK) and data analysis was performed using Q-view software (Quansys Biosciences, Logan, UT, USA).
4
Cytokine Profiling of BV2 Microglial Cells
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Following the 24-hour stimulation episode, the culture media of BV2 microglial cells was collected. Floating cells were spun at 1500 rpm and the supernatant was collected and stored at −80°C. Cytokine and chemokine concentrations were quantified using an enzyme-linked immunosorbant assay (ELISA)-based chemiluminescent assay, the Q-Plex Mouse cytokine – Screen (16-Plex) (Quansys Biosciences, Logan, UT) following the manufacturer’s protocol. The plate was read with a myECL Imager (Thermo Scientific, Whaltam, MA). The concentration of each cytokine was quantified with the Q-View Software (Quansys Biosciences). The following cytokines were detected: interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, monocyte chemoattractant protein-1 (MCP-1), interferon gamma (IFN-γ), tumor-necrosis factor alpha (TNFα), macrophage inflammatory proteins (MIP), granulocyte-macrophage colony-stimulating factor (GM-CSF), and regulated on activated, normal T cell expressed and secreted (RANTES).
5
Cytokine Profiling of PP242-Treated Cells
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CM by seeding 2x105 cells treated or not with PP242 (100 nM) were obtained as reported above. Cytokines were measured by using Q-Plex™ Array Human Angiogenesis Antigen (Quansys Biosciences, Logan, UT, USA) that detects ANG-2, FGF-2, HGF, IL-8, PDGF-BB, TIMP-1 and -2, TNF-α and VEGF, according to the manufacturer’s instructions. Secreted levels of cytokines were quantified through Q-View Software (Quansys Biosciences).
6
Isolation and Cytokine Profiling of PBMCs
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Following separation, the PBMCs were suspended in R5 medium supplemented with 20% native or heat-inactivated autologous serum. The suspension was then placed into the inner wells of 96-well microtiter plates, with each well containing approximately 5 × 105 cells. The plates were placed in a CO2 incubator (5% CO2) at 37 °C. Aliquots were collected at 45 min and 18 h after the start of the experiment. Additionally, baseline samples (“0 min samples”) were collected before the incubation. After collection, the samples were centrifuged (2500× g, 4 °C, 10 min), and the supernatants were stored at –80 °C until the C and cytokine assays were performed. Soluble terminal C complex (sTCC, sC5b-9) was measured with an ELISA kit from Svar Life Science AB (Malmö, Sweden), and for the measurement of cytokines, we used a Q-View™ LS chemiluminescent imager paired with Q-View™ Software, https://www.quansysbio.com/products-and-services/imaging/q-view-imager-ls/ (accessed on 18 March 2023), obtained from Quansys Biosciences (West Logan, UT, USA). The cytokine panel supplied by the company was the Human Cytokine Inflammation Panel 1 multiplex ELISA, measuring IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ, and TNF-α. All procedures followed the manufacturer’s instructions.
7
Multiplex ELISA for Mouse Cytokines
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Cell culture CM or BALF from mice were collected and analyzed using the Multiplex ELISA Kit for Mouse Cytokine Panel 1 (6-Plex, MEK1011, BOSTER) following the manufacturer’s instructions. All samples were diluted at a 1:2 ratio, and the results were analyzed using Q-View software (Quansys Biosciences).
8
Quantitative Cytokine Analysis of Wound Exudate
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In experimental group, the whole gel-like wound exudate was collected by abrasion with a sterile scalpel on day 1, day 5, and afterward weekly after trauma, whereas in control group, the exudate was left undisturbed. Biomaterial was collected in a small prechilled tube. After weighing, it was diluted in phosphate-buffered saline (1:10) and centrifuged at 2000 relative centrifugal force for 10 minutes at 4 °C. Supernatant was then transferred to nitrogen in 100 μL samples. Time from harvesting to freezing was kept under 30 minutes. For further experiments, samples were thawed on ice. Quantitative cytokine analysis was performed using a Q-Plex™ ELISA (Quansys Biosciences, Logan, UT) in accordance with the manufacturers’ description. Briefly, samples were loaded to the array plate containing immobilized antibodies spotted and blocked by the manufacturer. Antigen standards were loaded for an 8-point standard curve. After incubation, immobilized cytokines were labeled with biotinylated antibodies and then incubated with streptavidin-conjugated horse radish peroxidase. Antibodies and peroxidase were part of the Q-Plex™ ELISA kit. Directly after addition of the substrates, chemiluminescence was detected with the Fusion SL (Vilber Lourmat, Fisher Biotec, Australia). Signals were evaluated via Q-View™ software (Quansys Biosciences, Logan, UT).
9
Q-Plex Malaria Antigen Quantification
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The commercially-available Q-Plex™ Human Malaria assay (Quansys Biosciences, Logan, UT, USA) was used to detect and quantify four Plasmodium antigens and CRP in ethylenediaminetetraacetic acid-anticoagulated whole blood and heparinised plasma. The assay was performed according to manufacturer’s instructions and serially diluted protein standards from the kit were used to construct standard curves. Chemiluminescent imaging of each plate was captured on a Q-View™ Imager LS and analysed using Q-View™ software (Quansys Biosciences). Samples were run neat and diluted up to 100-fold until results were obtained in a reliable area of the standard curves. Several samples needing further dilution for HRP2 and CRP were assigned a value of the upper limit of quantification on their standard curve. Samples run neat that had antigen(s) below the detection limit were conservatively assigned a value corresponding to the assay’s lower limit of detection for that antigen(s).
10
Angiogenic Factor Profiling in Nicotine-Treated Cells
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Angiogenic factor profiling was performed using a multiplex ELISA array (Quansys Biosciences, Logan, UT), which can quantify the expression levels of 9 human angiogenic factors (ANG-2, FGF-2, HGF, PDGF, IL-8, TIMP-1, TIMP-2, TNFα and VEGF). Briefly, 2 × 105 cells were seeded on a 60-mm dish and treated with nicotine and/or cotinine for 7 days. Supernatant was collected at Day 7, subjected to the multiplex ELISA array, and incubated at room temperature for 1 hour. After washing with the Wash Buffer for 3 times, Detection Mix was added to the plate and incubated at room temperature for 1 hour. After 3-time washing, horse radish peroxidase-conjudated Streptavidin was applied and incubated at room temperature for 15 minutes. After 6-time washing, the substrate was added, and the plate was imaged immediately using the ChemiDocTM XRS+ system (BioRad, Hercules, CA). The intensities of the spots were quantified and calculated by the Q-View software (Quansys Biosciences) according to the standard curves for each factor. Mean of the 6 repeats for each group was compared for the statistical significance.
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