2 3 dioxo 6 nitro 1 2 3 4 tetrahydrobenzo f quinoxaline 7 sulfonamide disodium salt nbqx

[2,3-³H]D-aspartate (specific activity 11.3 Ci/mmol) was from Perkin Elmer (Boston, MA, USA). (S)AMPA, pep2-SVKI, pep2-SVKE, 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX), and DL-threo-β-Benzyloxyaspartic acid (DL-t-BOA) were purchased by Tocris Bioscience (Bristol, UK). Horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies and glycine were from Sigma (Milan, Italy). Luminata Forte Western blotting detection system, guinea pig anti-vesicular glutamate transporters type 1 (VGLUT1) antibody and mouse anti-GluA3 monoclonal antibody (MAB5416) were purchased from Millipore (Temecula, CA, USA). Rabbit anti-GluA1 polyclonal antibody (ab86141) and rabbit anti-GluA2 monoclonal antibody (ab206293, EPR18115) were from Abcam (Cambridge, UK). Rabbit anti-GluA4 monoclonal antibody was from GeneTex (GTX62957, Irvine, USA). Donkey anti-rabbit AlexaFluor-647, goat anti guinea pig AlexaFluor-688, donkey anti-mouse AlexaFluor-647, and donkey anti-goat AlexaFluor-688 were from Life Technologies Corporation (Carlsbad, CA, USA). Anti-syntaxin 1A monoclonal mouse IgG, was purchased from Chemicon (CA;USA). Complement and C1q-depleted complement was from Gentaur (Kampenhout, Belgium).

Pilocarpine hydrochloride and γ-aminobutyric acid (GABA) was purchased from Sigma-Aldrich (Dorset, UK) and dissolved in saline on the day of experiment. 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX, 10 μM), DL-2-Amino-5-phosphonopentanoic (DL-AP5; 50 μM), picrotoxin (100 μM) and (2 S)-3-[[(1 S)-1-(3,4-Dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl) phosphinic acid (CGP55845; 1 μM), used in electrophysiological recordings in vitro to block AMPA, NMDA-, GABA_A- and GABA_B-receptors respectively, were all from Tocris Bioscience (Bristol, UK).

Trans-Cyclooct-2-en-L-Lysine (TCO*A; #SC-8008) was purchased from SiChem (Bremen, Germany). Pyrimidyl-Tetrazine-Alexa Fluor 647 (Pyr-Tet-AF647; #CLK-102), Pyr-Tet-ATTO-643 (Pyr-Tet-ATTO643; #CLK-101), H-Tet-Cy3 (#CLK-014-05), and H-Tet-Cy5 (#CLK-015-05) were purchased from Jena Bioscience (Jena, Germany). SNAP-Surface^® Alexa Fluor^® 488 (BG-AF488; #S9129S) was purchased from New England Biolabs. 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX; #1044) and Kainate (KA; #0222) were purchased from Tocris. l-Glutamic acid monosodium salt (Glu; #G1626) and doxycycline (#D1822) were purchased from Sigma.