LC-HRMS analysis was performed on a Thermo Scientific Velos Pro instrument equipped with HESI source and Dionex UltiMate 3000 chromatography system. Samples were deproteinised as described for TLC. Compounds were separated on a Kinetex 2.6 µm EVO C18 column (2.1 ? 100 mm, Phenomenex) using a linear gradient of acetonitrile (B) against 20 mM ammonium bicarbonate (A): 0 – 5 min 2% B, 5 – 33 min 2 – 15% B, 33 – 35 min 15 – 98% B, 35 – 40 min 98% B, 40 – 41 min 98 – 2% B, 41 – 45 min 2% B. The flow rate was 350 µl min-1 and column temperature was 40 °C. UV data were recorded at 254 nm. Mass data were acquired on the FT mass analyzer in negative ion mode with scan range m/z 150 – 1500 at a resolution of 30,000. Source voltage was set to 3.5 kV, capillary temperature was 350 °C, and source heater temperature was 250 °C. Data were analysed using Xcalibur (Thermo Scientific). Extracted ion chromatograms were smoothed using the Boxcar function at default settings.
Kinetex 2.6 m evo c18 column
Manufactured by Phenomenex
The Kinetex 2.6 µm EVO C18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of analytes. It features a 2.6 µm particle size and a C18 stationary phase, which provides efficient and high-resolution separations.
Automatically generated - may contain errors
3 protocols using kinetex 2.6 m evo c18 column
1
LC-HRMS Analysis of Compounds
2
Metabolite Profiling by LC-HRMS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis was performed on a Thermo Scientific Velos Pro instrument equipped with HESI source and Dionex UltiMate 3000 chromatography system. Compounds were separated on a Kinetex 2.6 µm EVO C18 column (2.1 × 100 mm, Phenomenex) using a linear gradient of 2–15% acetonitrile against 20 mM ammonium bicarbonate pH 8 (gradient start delay 5 min, gradient length 28 min) at a flow rate of 350 µl min−1 and column temperature of 40°C. Data were acquired on the FT mass analyzer in negative ion mode with scan range m/z 150–1500. Source voltage was set to 3.5 kV, capillary temperature was 350°C, and source heater temperature was 250°C.
3
LC-HRMS Analysis of Compounds
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!