Non essential amino acid (neaa)

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom

Non-essential amino acids are a group of amino acids that can be synthesized by the human body, and are not required to be obtained from the diet. These amino acids play a fundamental role in protein synthesis and various metabolic processes.

Automatically generated - may contain errors

Lab products found in correlation

Sodium pyruvate, by Merck Group (17 mentions) L glutamine, by Merck Group (12 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (11 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (10 mentions) Fetal bovine serum (fbs), by Merck Group (10 mentions) Penicillin streptomycin, by Merck Group (9 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions) L glutamine, by Thermo Fisher Scientific (6 mentions) Streptomycin, by Thermo Fisher Scientific (6 mentions) Penicillin, by Merck Group (5 mentions) Streptomycin, by Merck Group (5 mentions) Penicillin streptomycin solution, by Merck Group (4 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (4 mentions) Penicillin, by Thermo Fisher Scientific (4 mentions) Hepes, by Merck Group (3 mentions) Rpmi 1640 medium, by Welgene (3 mentions) Model 680 microplate reader, by Bio-Rad (3 mentions) Fetal bovine serum (fbs), by Welgene (3 mentions) Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Glutamine, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

Non-essential amino acids (905 citations) Non-essential amino acids (NEAA; (30 citations) Amino acids (83 citations)

59 protocols using non essential amino acid (neaa)

Differentiation of Bone Marrow Cells

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

MF patients’ or normal donors’ BM LDCs were cultured in conditions favoring differentiation to either fibrocytes or MSCs, as previously described [6 (link), 21 (link), 52 (link)]. To differentiate LDCs to fibrocytes BM LDCs were cultured in StemSpan serum-free medium (Stemcell Technologies, Vancouver, BC, Canada) supplemented with modified eagle medium (MEM), non-essential amino acid, insulin-transferrin-selenium, HEPES, glutamine, sodium pyruvate, penicillin, and streptomycin solutions (Sigma-Aldrich). To grow MSCs, BM LDCs were cultured in α-MEM supplemented with 20% FBS (Invitrogen, Waltham, MA, USA). All LDC-derived cultures were grown in Nunc Lab-Tek II CC2 chamber slides (Thermo Scientific, Waltham, MA, USA) at 37 °C in humidified atmosphere supplemented with 5% CO₂.

Manshouri T., Veletic I., Li P., Yin C.C., Post S.M., Verstovsek S, & Estrov Z. (2022). GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes. Cell Death & Disease, 13(5), 481.

+ Open protocol

+ Expand

Thawing and Culturing Tissue Explants

Check if the same lab product or an alternative is used in the 5 most similar protocols

The freezing tube was taken out from the freezer at -80˚C and rapidly placed in a water bath at 37˚C. The frozen tissue was thawed at 37˚C completely in 1 min in the water bath. Subsequently, the frozen solution was poured into a Petri dish together with the tissue. The tissue was then picked out with a syringe needle, washed in culture medium pre-heated at 37˚C 4-5 times, implanted into a six-well culture plate and cultured with 2 ml solution. The culture conditions were as follows: DMEM was used as the basic culture medium (Sigma-Aldrich; Merck KGaA), supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA), 1% non-essential amino acid (Sigma-Aldrich; Merck KGaA) and 100 µg/ml penicillin and streptomycin (Sigma-Aldrich; Merck KGaA), with a culture temperature of 37.5˚C, saturated humidity and a 5% CO₂ concentration. Under a fluorescence microscope, the growth of the tissue was detected using Hoechst 33342 fluorescence staining (Beijing Reagan Biotechnology Co., Ltd.), at 1 µg/ml, dyed at room temperature for 5 min. The growth rate of the tissue was calculated using the following equation: Growth rate=number of tissue blocks on which fibroblasts have grown after culture/total number of tissue blocks cultured after resuscitation x100%.

Wang S., Yuan X., Zhou J., Jin J., Zuo Q, & Li B. (2020). Comparison of the effects of three cryoprotectants on the cryopreservation of mouse subcutaneous tissue under different conditions. Experimental and Therapeutic Medicine, 20(4), 3285-3289.

+ Open protocol

+ Expand

Nutrient Deprivation Experiments in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

All nutrient deprivation experiments were performed in the absence of serum for 24 hours, unless otherwise indicated. Cells were plated in complete culture media, which was exchanged with nutrient-deprived media (described below) 1–3 days after cell seeding. Nutrient-deprived media was not changed throughout the course of the experiments, unless otherwise indicated. For non-essential amino acid deprivation experiments, MEM medium without glutamine (Sigma) was used, and 1X MEM non-essential amino acids (Sigma) and/or 4 mM glutamine (Corning) were added for the corresponding controls. For cystine deprivation experiments, DMEM medium without methionine, cystine and glutamine (Sigma) was used, and 0.030 g/L methionine (Sigma), 0.063 g/L cystine (Sigma) and/or 4 mM glutamine were added for the corresponding controls. For glutamine deprivation experiments, glutamine-free RPMI (Corning), glutamine-free DMEM (Corning) or glutamine-free IMDM (Sigma) was used. For leucine deprivation experiments, leucine-free RPMI (Crystalgen) or leucine-free DMEM (Crystalgen) was used. For glucose deprivation experiments, glucose-free RPMI (Crystalgen) or glucose-free DMEM (Corning) was used. In Figures S3D, S4A and S4B, SW1990 cells were starved of glutamine in the presence of dialyzed FBS for 24 hours (S4A and S4B) or 48 hours (S3D).

Lee S.W., Zhang Y., Jung M., Cruz N., Alas B, & Commisso C. (2019). EGFR/Pak Signaling Selectively Regulates Glutamine Deprivation-Induced Macropinocytosis. Developmental cell, 50(3), 381-392.e5.

+ Open protocol

+ Expand

Dopaminergic Differentiation of Neural Stem Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neural stem cells (NSCs) were maintained in a growth medium comprised of Neurobasal medium (Gibco), B-27 (Gibco), non-essential amino acid (Sigma-Aldrich), GlutaMAX (Gibco), and 10 ng/mL of fibroblast growth factor (FGF). Differentiation into a dopaminergic phenotype was carried out over 10 days in medium comprised of Neurobasal medium, 1X B27 supplements, 1X non-essential amino acids, 20 ng/mL brain-derived neurotrophic factor (BDNF), 20 ng/mL glial-derived neurotrophic factor (GDNF), and 20 ng/mL TGFb3.^{101 (link)} All growth factors were purchased from Peprotech (Rocky Hill, NJ). NSCs were grown in plates/flasks coated with Geltrex ™ (ThermoFisher Scientific, Carlsbad, CA). 50% of the differentiation media was replaced with fresh media every 72 hours.

Moritz A.E., Free R.B., Weiner W.S., Akano E.O., Gandhi D., Abramyan A., Keck T.M., Ferrer M., Hu X., Southall N., Steiner J., Aubé J., Shi L., Frankowski K.J, & Sibley D.R. (2020). Discovery, Optimization and Characterization of ML417: A Novel and Highly Selective D3 Dopamine Receptor Agonist. Journal of medicinal chemistry, 63(10), 5526-5567.

+ Open protocol

+ Expand

Vocal Fold Fibroblast Cell Culture

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human fibroblasts cell lines were derived from polyp, scar, and two age-matched normal vocal fold tissue samples based on protocols approved by the University of Wisconsin Health Sciences Institutional Review Board as previously described [8 (link),14 (link),15 (link)]. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, 0.01 mg/mL streptomycin sulfate and 1× non-essential amino acid (all from Sigma Inc, St Louis) at 37°C in atmosphere of 5% CO₂. Fibroblasts were used between passages five and nine.

King S.N., Berchtold C.M, & Thibeault S.L. (2014). Lipopolysaccharide responsiveness in vocal fold fibroblasts. Journal of Inflammation (London, England), 11, 42.

+ Open protocol

+ Expand

PBMC Isolation and Stimulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples were collected in tubes with lithium heparin (Vacutainer®) and diluted with an equal volume of warm PBS (Gibco, Invitrogen, Massachusetts). PBMCs were isolated by centrifuging at 800 g for 20 min at 18–20℃ on Biocoll separating solution (Biochrom AG, Germany). PBMCs were washed three times, and the cell pellet was resuspended in complete medium [RPMI 1640 with HEPES 25 mM and l‐Glutamine (Gibco, Life Technologies Ltd, UK), supplemented with 10 ml/L Penicillin‐Streptomycin USA, 50 μl/L 1 M β‐mercaptoethanol, 20 ml/L l‐Glutamine plus MEM Vitamin, 20 ml/L Non‐essential Amino Acid, Sodium Pyruvate and 10% heat‐inactivated FBS (all from Sigma‐Aldrich, Germany)]. The suspension was seeded in a flat‐bottom 48‐well tissue plate (Corning Incorporated, Costar, New York), with 5 × 10⁵ viable cells per well (500 μl). PBMCs were cultured in duplicates either with complete medium alone (unstimulated control) or with one of the following stimulants: 4 μg/ml Resiquimod (R848), 10 μg/ml Zymosan (InvivoGen, France), 10 μg/ml Phytohemagglutinin (PHA‐M), 20 μg/ml Polyinosinic–polycytidylic acid potassium salt (Poly I:C), (Sigma‐Aldrich, Germany), at 37℃, 5% CO₂. Cultures were harvested after 48 h and, after centrifugation at 600 g for 5 min, supernatants were stored at −80℃ until analysis.

Maurer D.J., Liu C., Xepapadaki P., Stanic B., Bachert C., Finotto S., Gao Y., Graser A., Jartti T., Kistler W., Kowalski M., Lukkarinen H., Pasioti M., Tan G., Villiger M., Zhang L., Zhang N., Akdis M., Papadopoulos N.G, & Akdis C.A. (2021). Physical activity in asthma control and its immune modulatory effect in asthmatic preschoolers. Allergy, 77(4), 1216-1230.

+ Open protocol

+ Expand

In Vitro Immune Cell Stimulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Thymocytes and LN cells were cultured in RPMI 1640 medium (Welgene) containing 10% FBS (Gemini), L-glutamine, 100,000 U/ml penicillin plus 100 mg/ml streptomycin (GIBCO), nonessential amino acid (Sigma-Aldrich), sodium pyruvate (Sigma-Aldrich), and β-mercaptoethanol (GIBCO). They were stimulated with recombinant human IL-2 (10 ng/ml), murine IL-4 (20 ng/ml), IL-7 (10 ng/ml), IL-9 (100 ng/ml), IL-15 (100 ng/ml), IL-21 (100 ng/ml), or IL-6 (10 ng/ml; all from PeproTech) or stimulated in presence of sodium azide (Sigma-Aldrich) in a 37°C or 4°C humidified incubator.

Goh T.S., Jo Y., Lee B., Kim G., Hwang H., Ko E., Kang S.W., Oh S.O., Baek S.Y., Yoon S., Lee J.S, & Hong C. (2017). IL-7 Induces an Epitope Masking of γc Protein in IL-7 Receptor Signaling Complex. Mediators of Inflammation, 2017, 9096829.

+ Open protocol

+ Expand

Isolation and Culture of Menstrual Blood-derived Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

For study purposes, samples were collected from six woman aged 25 ~ 35 years old with the informed consent approved by the Ethics Committee of Shengjing Hospital affiliated to China Medical University. MenSCs were then isolated and cultured accordingly as our previous study reported [30 (link)]. In brief, MenSCs were purified from menstrual blood by density gradient centrifugation with Ficoll-Paque solution (GE Healthcare Bio-sciences, Uppsala, Sweden). The enriched MenSCs were cultured in 25-cm² culture flask with DMEM/F12 medium (Hyclone, Logan, UT, USA) supplemented with 10% FBS (Gibco, Waltham, MA, USA), 2 mM L-glutamine (Sigma-Aldrich), 1% non-essential amino acid (Sigma-Aldrich) and 1% penicillin-streptomycin (Sigma-Aldrich) in a humidified incubator under 37 °C with 5% CO₂.

Feng P., Li P, & Tan J. (2018). Human Menstrual Blood-Derived Stromal Cells Promote Recovery of Premature Ovarian Insufficiency Via Regulating the ECM-Dependent FAK/AKT Signaling. Stem Cell Reviews, 15(2), 241-255.

+ Open protocol

+ Expand

TC-1 Cell-Based Murine Model for Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Female C57BL/6 (6–8 weeks old) mice were used in this study and purchased from Animal Resources Centre (Perth, Western Australia). TC-1 cells (murine C57BL/6 lung epithelial cells transformed with HPV-16 E6/E7 and ras oncogenes).29 (link) TC-1 cells were cultured and maintained at 37 °C/5% CO₂ in RPMI 1640 medium (Gibco) supplemented with 10% heat inactivated fetal bovine serum (Gibco) and 1% nonessential amino acid (Sigma-Aldrich). The animal experiments were approved by the University of Queensland Animal Ethics committee (DI/034/11/NHMRC) and (UQDI/327/13/NHMRC) in accordance with National Health and Medical research Council (NHMRC) of Australia guidelines.

Hussein W.M., Liu T.Y., Maruthayanar P., Mukaida S., Moyle P.M., Wells J.W., Toth I, & Skwarczynski M. (2016). Double conjugation strategy to incorporate lipid adjuvants into multiantigenic vaccines †Electronic supplementary information (ESI) available: Structures, spectroscopic data with 1H and C13 NMR spectra, HPLC profiles and MS spectra. See DOI: 10.1039/c5sc03859f. Chemical Science, 7(3), 2308-2321.

+ Open protocol

+ Expand

Profiling B Cell Clonal Lineages

Check if the same lab product or an alternative is used in the 5 most similar protocols

We obtained whole blood drawn from volunteers at the Stanford Blood Center and prepared enriched B cell fractions using the RosetteSep kit (StemCell Technologies, Cambridge, MA) according to manufacturer’s instructions. We sorted CD19+ IgM+ cells and cultured them at 5 × 10⁵ cells/ml for 5 days at 37 C and 5% CO₂ in RPMI 1640 with L-glutamine (ThermoFisher) supplemented with 10% fetal bovine serum, 10 mM HEPES pH 7.4, 0.1 mM non-essential amino acid (Sigma-Aldrich, St. Louis, MO), 1 mM sodium pyruvate, 100 µ/ml penicillin, 100 µg/ml streptomycin (ThermoFisher), 40 µg/ml apo-transferrin, 500 ng/µl multimeric CD40 ligand (Miltenyi Biotec, San Diego, CA), 200 ng/ml IL-4 (Sigma-Aldrich), and 200 ng/ml IL-10 (Sigma-Aldrich). We extracted RNA from the cells using the RNeasy Micro Kit (Qiagen) according to manufacturer’s instructions, but omitting the DNase digestion step. We then prepared sequencing libraries using 24.5 ng of total RNA as input as described above, except that PCR products were purified using Ampure XP beads at a 0.65:1 ratio instead of a 1:1 ratio before pooling for multiplexed sequencing. We processed the sequences, reconstructed the clonal lineage histories, and measured correlations between the class switch fates of related cells as described above.

Horns F., Vollmers C., Croote D., Mackey S.F., Swan G.E., Dekker C.L., Davis M.M, & Quake S.R. (2016). Lineage tracing of human B cells reveals the in vivo landscape of human antibody class switching. eLife, 5, e16578.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!