Dneasy plant maxi kit

Manufactured by Qiagen

Sourced in United States, Germany, Netherlands, Spain

The DNeasy Plant Maxi Kit is a laboratory equipment designed for the efficient extraction and purification of genomic DNA from a wide range of plant materials. It utilizes a silica-membrane-based technology to provide high-quality DNA suitable for various downstream applications.

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Close spelling variants of this product

DNeasy kit (1236 citations) DNeasy Plant Kit (193 citations) Maxi Kit (58 citations) DNeasy® Plant Maxi Kit (24) (6 citations) DNeasy® Plant Mini or Maxi kit (4 citations) DNeasy Plant Maxi (2 citations) DNeasy Plant (2 citations)

105 protocols using dneasy plant maxi kit

Genomic DNA Extraction and PCR Amplification

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Extraction of genomic DNA was performed from young leaf tissue. The leaf material was grounded under liquid nitrogen and subsequently used for DNA isolation with the DNeasy Plant Maxi Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocols. The purified DNA was quality checked via gel electrophoresis and quantified using a NanoDrop spectrophotometer (Peqlab, Erlangen, Germany). Amplicons were amplified by long range PCR (98°C 30 sec, 15 cycles of 10 sec 98°C, 30 sec 72°C– 57°C, 5 min 72°C, 25 cycles 10 sec 98°C, 30 sec 58°C, 5 min 72°C and finally 2 min 72°C).
Target gene sequences were amplified from 37 individuals of the mapping population GF.GA-47-42 x ‘Villard Blanc’ including the parental lines and 35 F₁ individuals with early, intermediate and, late flowering time phenotypes (S3 Table).

Kamal N., Ochßner I., Schwandner A., Viehöver P., Hausmann L., Töpfer R., Weisshaar B, & Holtgräwe D. (2019). Characterization of genes and alleles involved in the control of flowering time in grapevine. PLoS ONE, 14(7), e0214703.

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Extraction of Fungal DNA and RNA

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Cadophora sp. DSE1049 and P. macrospinosa DSE2036 were maintained in modified Melin-Norkrans (MMN) liquid medium^{51 (link)} containing 3 g/L glucose and were grown as a free-living vegetative mycelium for two weeks at room temperature in the dark. For harvesting, the mycelium was dried on filter paper, flash-frozen in liquid nitrogen and ground into a powder. DNA was extracted from 2.5 g mycelium using the DNeasy Plant Maxi kit (Qiagen) according to the manufacturer’s instructions (doing on-column RNase treatment). In addition, total RNA was isolated from 0.5 g mycelium using the RNeasy Plant Midi Kit (Qiagen) according to the manufacturer’s instructions, including DNase treatment.

Knapp D.G., Németh J.B., Barry K., Hainaut M., Henrissat B., Johnson J., Kuo A., Lim J.H., Lipzen A., Nolan M., Ohm R.A., Tamás L., Grigoriev I.V., Spatafora J.W., Nagy L.G, & Kovács G.M. (2018). Comparative genomics provides insights into the lifestyle and reveals functional heterogeneity of dark septate endophytic fungi. Scientific Reports, 8, 6321.

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DNA Extraction and Genotyping of Grapevine Cultivars

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Deoxyribonucleic Acid (DNA) was extracted from 1 g of young leaves (with main rib less than 2 cm long) using DNeasy Plant Maxi Kit (Qiagen, Germany) following the manufacturer’s instructions. The concentration and quality of the DNA were checked using the Agilent® 2100 bioanalyzer system (Agilent, Santa Clara, CA, United States). The population was genotyped using the Illumina® 18 K SNP Infinium chip (18,071 SNP markers; [106 ]). Results were visualized and manually edited when necessary using the Illumina® Genome Studio software version 2011.1 [118 ]. The SNP markers that were monomorphic (55 % of the total markers), multilocus or with an ambiguous pattern (8 %), highly distorted or with a minor allele frequency < 10 % (1 %), were discarded. The remaining 6,000 SNP markers passing these filters were used to build the genetic maps, out of which 2,727 and 4,284 were heterozygous in Picovine and Ugni Blanc flb, respectively.

Houel C., Chatbanyong R., Doligez A., Rienth M., Foria S., Luchaire N., Roux C., Adivèze A., Lopez G., Farnos M., Pellegrino A., This P., Romieu C, & Torregrosa L. (2015). Identification of stable QTLs for vegetative and reproductive traits in the microvine (Vitis vinifera L.) using the 18 K Infinium chip. BMC Plant Biology, 15, 205.

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Gymnosperm DNA Isolation and Cytogenetics

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Needles and seeds of eleven gymnosperm accessions have been obtained from the Forest Botanical Garden of Tharandt (Technische Universität Dresden) and the Staatsbetrieb Sachsenforst (Table 1). DNA was isolated from 2 g of homogenized material from frozen needles using the DNeasy Plant Maxi Kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions. To allow for a more efficient elution of conifer DNA, the incubation time during the elution step was increased to 10 min. Purified DNA was eluted into water instead of the provided AE buffer.
For cytogenetics, we have used primary root tips from seeds of L. decidua (obtained as selected material for propagation from Staatsdarre Flöha, Partie number 1846, ELA/83704) and L. kaempferi seeds (obtained from Niedersächsische Landesforsten, provenance number 83901), as well as root tips from L. × eurolepis plantlets (clone 56.012.15) obtained from somatic embryogenic cultures from Madlen Walter and Kurt Zoglauer from the Humboldt Universität zu Berlin.

Heitkam T., Schulte L., Weber B., Liedtke S., Breitenbach S., Kögler A., Morgenstern K., Brückner M., Tröber U., Wolf H., Krabel D, & Schmidt T. (2021). Comparative Repeat Profiling of Two Closely Related Conifers (Larix decidua and Larix kaempferi) Reveals High Genome Similarity With Only Few Fast-Evolving Satellite DNAs. Frontiers in Genetics, 12, 683668.

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Rhizomucor pusillus Strain Genomic DNA Extraction

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Example 7

Rhizomucor pusillus strain NN046782 was inoculated onto a PDA plate and incubated for 3 days at 45° C. in the darkness. Several mycelia-PDA plugs were inoculated into 500 ml shake flasks containing 100 ml of FG4 medium. The flasks were incubated for 3 days at 45° C. with shaking at 160 rpm. The mycelia were collected by filtration through MIRACLOTH® (Calbiochem, La Jolla, Calif., USA) and frozen under liquid nitrogen. Frozen mycelia were ground, by a mortar and a pestle, to a fine powder, and genomic DNA was isolated using DNeasy® Plant Maxi Kit (QIAGEN Inc., Valencia, Calif., USA) following the manufacturer's instruction.

US10253342B2. Polypeptides having catalase activity and polynucleotides encoding same (2019-04-09). Novozymes Inc. [US]. Inventors: Ye Liu [CN], Junxin Duan [CN], Yu Zhang [CN], Lan Tang [CN].

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Thermophilic Fungus DNA Extraction

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Example 1

Thermoascus aurantiacus strain NN044936 was inoculated onto a PDA plate and incubated for 3 days at 45° C. in the darkness. Several mycelia-PDA plugs were inoculated into 500 ml shake flasks containing 100 ml of YPG medium. The flasks were incubated for 3 days at 45° C. with shaking at 160 rpm. The mycelia were collected by filtration through MIRACLOTH® (Calbiochem, La Jolla, Calif., USA) and frozen in liquid nitrogen. Frozen mycelia were ground, by a mortar and a pestle, to a fine powder, and genomic DNA was isolated using DNeasy® Plant Maxi Kit (QIAGEN Inc., Valencia, Calif., USA) following the manufacturer's instruction.

US11788078B2. Processes for producing ethanol (2023-10-17). Novozymes A/S [DK]. Inventors: Tianqi Sun [CA], Ming Li [CN], Junxin Duan [CN].

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Genomic DNA Analysis for J1-1 Gene Integration

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To analyze the genomic DNA for integration of the J1-1 gene, pepper genomic DNA was isolated using a DNeasy Plant Maxi Kit (Qiagen, Hilden, Germany) as described by the manufacturer. For Southern blot analysis, 15 µg of each DNA sample was digested with EcoRI and separated on a 1.0% (w/v) agarose gel. The digested DNA was then transferred to a nylon membrane and hybridized with HPT or J1-1 gene probes that was labeled with [α³²P] dCTP using the Rediprime II Random Prime Labeling System (Amersham Biosciences, UK). After hybridization, the membranes were exposed at −80°C on Kodak XAR-5 film (Kodak, Rochester, NY) using an intensifying screen.
For Northern blot analysis, total RNA was extracted from the pepper fruits using a RNeasy Plant Kit (Qiagen, Hilden, Germany). 10 µg of the total RNA was separated on 1.2% denaturing agarose gels and blotted onto a Hybond N⁺ membrane (GE Healthcare, Buchinghamshire, UK). The blots were then hybridized with [α³²P] dCTP-labeled respective probes that were amplified by PCR. The primers used for probes were shown in Table S1.

Seo H.H., Park S., Park S., Oh B.J., Back K., Han O., Kim J.I, & Kim Y.S. (2014). Overexpression of a Defensin Enhances Resistance to a Fruit-Specific Anthracnose Fungus in Pepper. PLoS ONE, 9(5), e97936.

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Molecular Genotyping of Vernalization and Photoperiod Genes

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Total DNA was extracted from leaves using a DNeasy Plant Maxi Kit (Qiagen, Germany) following the manufacturer’s protocol. We used polymerase chain reaction (PCR) primers (Supplemental Table 3) that had been shown to identify the alleles of Vrn-1 and Ppd-1 homoeologues in previous studies, and we amplified DNA by PCR using a T100 thermal cycler (Bio-Rad Laboratories Inc., Hercules, CA, USA) and GoTaq DNA polymerase (Promega Corp., Madison, WI, USA). The PCR conditions were as follows: denaturation at 95°C for 1 min, followed by 35 cycles of the denaturation at 95°C for 30 s, annealing for 30 s, extension at 72°C for 30 s, and then final extension at 72°C for 5 min. Information on the primer sets used in this study is presented in Supplemental Table 3. The amplicons were separated on a 2.0% agarose gel and visualized using SYBR Safe DNA Gel Stain (Invitrogen, Carlsbad, CA, USA), or separated by a capillary electrophoresis system (LabChip GX Touch HT, PerkinElmer, Inc., Waltham, MA, USA) with a DNA5K/RNA/CZE chip (PerkinElmer). Allele names of the Vrn-1 and Ppd-1 genes were determined according to Chen et al. (2018) (link).

Mizuno N., Matsunaka H., Yanaka M., Nakata M., Nakamura K., Nakamaru A., Kiribuchi-Otobe C., Ishikawa G., Chono M., Hatta K., Fujita M, & Kobayashi F. (2022). Allelic variations of Vrn-1 and Ppd-1 genes in Japanese wheat varieties reveal the genotype-environment interaction for heading time. Breeding Science, 72(5), 343-354.

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Microbial Community DNA Extraction

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Total DNA was extracted from the rhizosphere and bulk soil with the PowerSoil® DNA Isolation Kit (MoBio Inc., USA), starting from 0.5 g of each sample. For the root samples, total DNA was extracted from one gram of sterilized and grinded material^{30 (link)} using the DNeasy Plant Maxi Kit (Qiagen, Germany).

Mosqueira M.J., Marasco R., Fusi M., Michoud G., Merlino G., Cherif A, & Daffonchio D. (2019). Consistent bacterial selection by date palm root system across heterogeneous desert oasis agroecosystems. Scientific Reports, 9, 4033.

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Talaromyces emersonii Genomic DNA Extraction

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Example 4

Talaromyces emersonii strain NN051602 was inoculated onto a PDA plate and incubated for 3 days at 45° C. in the darkness. Several mycelia-PDA plugs were inoculated into 500 ml shake flasks containing 100 ml of YPG medium. The flasks were incubated for 3 days at 45° C. with shaking at 160 rpm. The mycelia were collected by filtration through MIRACLOTH® (Calbiochem, La Jolla, Calif., USA) and frozen in liquid nitrogen. Frozen mycelia were ground, by a mortar and a pestle, to a fine powder, and genomic DNA was isolated using DNeasy® Plant Maxi Kit (QIAGEN Inc., Valencia, Calif., USA) following the manufacturer's instruction.

US11180746B2. Polypeptides having alpha-amylase activity and polynucleotides encoding same (2021-11-23). NOVOZYMES A/S [DK]. Inventors: Tianqi Sun [CN], Ming Li [CN], Junxin Duan [CN].

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