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C11440 5b camera

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The C11440-5B is a high-sensitivity camera manufactured by Hamamatsu Photonics. It features a large-format CMOS image sensor and provides high quantum efficiency. The camera is designed for scientific and industrial applications requiring high-performance imaging capabilities.

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Lab products found in correlation

Nanosight lm10, by Malvern Panalytical (5 mentions) Nta nanosight lm10, by Malvern Panalytical (3 mentions) Nta analytical software version 2, by Malvern Panalytical (1 mentions) Jem 1400, by JEOL (1 mentions) Veleta, by EMSIS (1 mentions)

9 protocols using c11440 5b camera

1

Cryo-EM and Flow Cytometry Analysis of Extracellular Vesicles

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The morphology of the isolated EVs was assessed by cryo-EM, as described previously [38 (link)]. The initial volumes of plasma or blood for the study of EV using the cryo-EM were 10 mL.
The size and concentration of the isolated EVs were evaluated by NTA using the NanoSight® LM10 (Malvern Instruments, UK) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan), at several dilutions according to the manufacturer’s instructions. Each sample was measured in triplicate, with a camera setting of 15, an acquisition time of 60 s, and a detection threshold setting of 5. At least 200 completed tracks were analyzed per video. NTA analytical software version 2.3 was used for data analysis and capture.
The immunocytochemical identification of exosomes from plasma and total blood with monoclonal antibodies to tetraspanin CD9 was performed as described previously [45 (link)]. Quantitative analysis of the exosomal tetraspanines on the surface of the isolated EVs was carried out using flow cytometry, as described previously [46 (link)]. Flow cytometry was performed on the Cytoflex (Becman Coulter, USA), using the CytExpert 2.0 Software. The MFI of the stained exosomes was analyzed and compared to the isotype control (BD bioscience, USA).
Konoshenko M., Sagaradze G., Orlova E., Shtam T., Proskura K., Kamyshinsky R., Yunusova N., Alexandrova A., Efimenko A, & Tamkovich S. (2020). Total Blood Exosomes in Breast Cancer: Potential Role in Crucial Steps of Tumorigenesis. International Journal of Molecular Sciences, 21(19), 7341.
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2

Nanoparticle Tracking Analysis of GF-EVs

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The size of GF-EVs and their concentration in suspensions were determined by NTA using the NanoSight LM10 (Malvern Instruments, UK) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan). Recording and data analysis were performed using the NTA software 2.3. The following parameters were evaluated during the analysis of recordings monitored for 60 s: the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension.
Garaeva L., Kamyshinsky R., Kil Y., Varfolomeeva E., Verlov N., Komarova E., Garmay Y., Landa S., Burdakov V., Myasnikov A., Vinnikov I.A., Margulis B., Guzhova I., Kagansky A., Konevega A.L, & Shtam T. (2021). Delivery of functional exogenous proteins by plant-derived vesicles to human cells in vitro. Scientific Reports, 11, 6489.
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3

Nanoparticle Tracking Analysis of GF-EVs

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The size of GF-EVs and their concentration in suspensions were determined by NTA using the NanoSight LM10 (Malvern Instruments) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan). Recording and data analysis were performed using the NTA software 2.3. The following parameters were evaluated during the analysis of recordings monitored for 60 s: the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension.
Garaeva L., Kamyshinsky R., Kil Y., Varfolomeeva E.Y., Garmay Y., Ланда С.Б., Burdakov V., Myasnikov A.G., Kagansky A., Vinnikov I.A., Komarova E.Y., Margulis B.A., Guzhova I.V., Konevega A.L., & Shtam T. (2020). Delivery of Functional Exogenous Proteins by Plant Vesicles to Human Cells in Vitro.
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4

Nanoparticle Tracking Analysis of Extracellular Vesicles

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The size and concentration of EVs were determined by NTA using the NTA NanoSight LM10 analyzer, equipped with a 405 nm laser (Nano-Sight, Malvern Instruments) and a C11440-5B camera (Hamamatsu Photonics K.K.). Before NTA measuring, an aliquot of the isolated EVs was thawed at room temperature and diluted with deionized water 1,000, 10,000, 100,000 times and injected in the sample chamber with sterile syringe. Recording and data analysis were performed using the NTA software 2.3. Particles were captured by recording 30s video at room temperature and following parameters: camera level—16, low threshold—0, high threshold—2015. Received videos (750 frames captured with 10–40 particles/per frame) were analyzed at detection threshold value of 8 and minimal expected size. The measurements were carried out three times. Software used identifies and tracks individual nanoparticles moving under Brownian motion and relates the movement to a particle size according to the Stokes-Einstein formula. So, the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension were determined.
Miroshnikova V.V., Dracheva K.V., Kamyshinsky R.A., Yastremsky E.V., Garaeva L.A., Pobozheva I.A., Landa S.B., Anisimova K.A., Balandov S.G., Hamid Z.M., Vasilevsky D.I., Pchelina S.N., Konevega A.L, & Shtam T.A. (2023). Cryo-electron microscopy of adipose tissue extracellular vesicles in obesity and type 2 diabetes mellitus. PLOS ONE, 18(2), e0279652.
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5

Nanoparticle Tracking Analysis of GEVs and TEVs

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The sizes and concentrations of GEVs and TEVs in suspensions were determined by NTA using the NanoSight® LM10 (Malvern Instruments, Malvern, UK) with a UV laser (45 mW at 405 nm) using a C11440-5B camera (Hamamatsu photonics KK, Shizuoka, Japan). Recording and data analysis were performed using the NTA software 2.3.
The NTA of GEVs and TEVs was performed by diluting the samples in Milli-Q water between 1000 and 100,000-fold at 25 °C (Camera level: 16, Low threshold: 0, High threshold: 2.015) with a minimum expected size of 30 nm. The following parameters were evaluated during the analysis of records monitored for 30 s: average hydrodynamic diameter, mode of distribution, and concentration of vesicles in the suspension.
Kilasoniya A., Garaeva L., Shtam T., Spitsyna A., Putevich E., Moreno-Chamba B., Salazar-Bermeo J., Komarova E., Malek A., Valero M, & Saura D. (2023). Potential of Plant Exosome Vesicles from Grapefruit (Citrus × paradisi) and Tomato (Solanum lycopersicum) Juices as Functional Ingredients and Targeted Drug Delivery Vehicles. Antioxidants, 12(4), 943.
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6

Characterizing Extracellular Vesicle Size and Concentration

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The size of EVs and their concentration were determined using the NTA NanoSight® LM10 (Malvern Instruments) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan). Recording and data analysis were performed using the NTA software 2.3. To optimize the measurement mode, the samples of isolated vesicles were diluted 1:100, 1:1000, or 1:10,000 by PBS. In the selected dilution, each sample was measured in triplicate. The following parameters were evaluated during the analysis of recordings monitored for 60 s: the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension.
Komarova E.Y., Suezov R.V., Nikotina A.D., Aksenov N.D., Garaeva L.A., Shtam T.A., Zhakhov A.V., Martynova M.G., Bystrova O.A., Istomina M.S., Ischenko A.M., Margulis B.A, & Guzhova I.V. (2021). Hsp70-containing extracellular vesicles are capable of activating of adaptive immunity in models of mouse melanoma and colon carcinoma. Scientific Reports, 11, 21314.
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7

Nanoparticle Tracking Analysis of EVs

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The size of EVs and their concentration were determined using the NTA NanoSight® LM10 (Malvern Instruments) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan). Recording and data analysis were performed using the NTA software 2.3. To optimize the measurement mode, the samples of isolated vesicles were diluted 1:100, 1:1000, or 1:10,000 by PBS. In the selected dilution, each sample was measured in triplicate. The following parameters were evaluated during the analysis of recordings monitored for 60 s: the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension.
Komarova E.Y., Суезов Р.В., Nikotina A.D., Aksenov N., Garaeva L.А., Shtam T., Zhakhov A., Martynova M.G., Добродумов А.В., Istomina M., Ischenko A., Margulis B.A., & Guzhova I.V. (2021). Hsp70-containing Extracellular Vesicles are Capable of Activating of Adaptive Immunity in Models of Mouse Melanoma and Colon Carcinoma.
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8

Characterization of Extracellular Vesicles by TEM and NTA

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Morphology of the isolated extracellular vesicles (EVs) was assessed by transmission electron microscopy (TEM) as described previously [26 (link)]. Specific markers for exosomes, CD9 or CD63 were detected as described earlier [26 (link)] using 10 μL of vesicles mixed with 10 μL of 0.5% bovine serum albumin in PBS and 3 μL (100 μg/mL) of corresponding monoclonal antibodies (Abcam, Cambridge, UK). All grids were examined in JEM 1400 (Jeol, Tokyo, Japan) TEM supplied with digital camera Veleta (EM SIS, Muenster, Germany). The measurements were made directly on the camera screen using iTEM (EM SIS, Muenster, Germany) software, version 5.2.
The size and concentration of the isolated EVs were determined by NTA using the NanoSight® LM10 (Malvern Instruments, Malvern, Worcestershire, UK) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Shizuoka, Japan), at several dilutions according to the manufacturer’s instructions. Each sample was measured in triplicate, with a camera setting of 15, an acquisition time of 60 s, and a detection threshold setting of 5. At least 200 completed tracks were analyzed per video. NTA analytical software version 2.3 (Malvern Instruments, Malvern, Worcestershire, UK) was used for data analysis and capture.
Tutanov O., Shtam T., Grigor’eva A., Tupikin A., Tsentalovich Y, & Tamkovich S. (2022). Blood Plasma Exosomes Contain Circulating DNA in Their Crown. Diagnostics, 12(4), 854.
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9

Nanoparticle Tracking Analysis of EVs

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The size and concentration of EVs were determined by NTA using the NTA NanoSight® LM10 (Malvern Instruments) analyzer, equipped with a blue laser (45 mW at 488 nm) and a C11440-5B camera (Hamamatsu Photonics K.K., Japan). Recording and data analysis were performed using the NTA software 2.3. The following parameters were evaluated during analysis of recording monitored for 60 s: the average hydrodynamic diameter, the mode of distribution, the standard deviation, and the concentration of vesicles in the suspension. Before NTA measuring, an aliquot of the isolated vesicles was thawed at room temperature and diluted with deionized water in 10, 100, 1000 times. The measurements were performed at least three times.
Emelyanov A., Shtam T., Kamyshinsky R., Garaeva L., Verlov N., Miliukhina I., Kudrevatykh A., Gavrilov G., Zabrodskaya Y., Pchelina S, & Konevega A. (2020). Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid. PLoS ONE, 15(1), e0227949.
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