MCF-7, and T47D cell lines were maintained in RPMI-1640 medium containing 10 % FBS, 100U/mL penicillin and streptomycin at 37 °C in 5 % CO2. The cells were placed in airtight chambers (Nu Aire, Plymouth, MN, USA). At the beginning of each experiment, the cells were resuspended in the medium at a density of 2.5 × 105 cells/mL. Cells were treated with Tam at 25 μM, MSM at 300 mM and/or a combination of both (Tam at 15 μM and MSM at 200 mM).
Airtight chambers
Manufactured by NuAire
Sourced in United States
Airtight chambers are enclosed spaces designed to maintain a controlled environment. They are used to isolate samples or processes from external factors, providing a secure and regulated atmosphere. These chambers offer a seal to prevent the exchange of air or other substances between the interior and exterior.
Automatically generated - may contain errors
Lab products found in correlation
Mitochondria isolation kit, by Thermo Fisher Scientific (1 mentions)
Dioc6, by Merck Group (1 mentions)
Coomassie bradford protein assay kit, by Thermo Fisher Scientific (1 mentions)
β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
Qiaprep spin miniprep kit, by Qiagen (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Bcl 2, by Santa Cruz Biotechnology (1 mentions)
Bcl xl, by Santa Cruz Biotechnology (1 mentions)
Oligo dt, by Bioneer (1 mentions)
4 protocols using airtight chambers
1
Breast Cancer Cell Line Treatment Effects
2
Nobiletin Effects on Breast Cancer Cell Lines
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ER+ cell lines, MCF-7 and T47D cell lines, triple negative cell line, MDA-MB-231, Her2+ cell line, and SKBR3 were cultured and maintained in RPMI-1640 medium containing 10% FBS, 100 U/mL penicillin at 37 °C in 5% CO2. The endothelial cell line HUVEC was maintained in EBM-2 endothelial growth basal media. The cells were placed in airtight chambers (Nu Aire, Plymouth, MN, USA). At the beginning of each experiment, the cells were counted depending on the experiment and were re-suspended in the medium. Cells were treated with nobiletin at different concentrations according to the experiments.
3
Nobiletin Impacts on Breast Cancer Cells
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ER+ cell line, MCF-7 cell line and triple negative cell line, MDA-MB-231 were maintained in RPMI-1640 medium containing 10% FBS, 100 U/mL penicillin at 37 °C in 5% CO2. Endothelial cell line, HUVEC was maintained in EBM-2 endothelial growth basal media. The cells were placed in airtight chambers (Nu Aire, Plymouth, MN, USA). At the beginning of each experiment, the cells were counted depends on experiments and re-suspended in the medium. Cells were treated with nobiletin at different concentration according to the experiments. Media for tumor sphere formation was made by adding the growth supplements B27, EGF and bFGF to DMEM/F12 media.
4
Methylsulfonylmethane Induces Apoptosis in Gingival Cancer Cells
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Antibodies and reagents. RPMI-1640 medium, 10% fetal bovine serum (FBS), and 0.05% trypsin-ethylenediaminetetraacetic acid were purchased from Gibco-BRL (Grand Island, NY, USA). BAX, BCL-2, BCL-XL, CASPASE-3, β-actin antibodies and secondary antibodies (rabbit and goat anti-mouse IgG-horse radish peroxidase) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). An enhanced chemiluminescence detection kit (ECL plus), RT-PCR Premix kits, oligo d(T), and BCL-2, BCL-XL, BAX and 18S primers for RT-PCR were purchased from Bioneer (Daejeon, Korea). DiOC6 was obtained from Sigma (St. Louis, MO, USA). A mitochondria isolation kit and a Coomassie (Bradford) Protein Assay Kit were purchased from Thermo Scientific (Waltham, MA, USA). Restore Western Blot Stripping Buffer and NE-PER kits were purchased from Pierce (Rockford, IL, USA). An RNeasy mini kit and Qiaprep spin mini prep kits were purchased from Qiagen (Hilden, Germany). MSM was purchased from Fluka/Sigma Co. (St. Louis, MO, USA).
Cell culture and treatment. The gingival cancer cell line YD-38 was maintained in RPMI-1640 containing 10% FBS and 1% penicillin/streptavidin at 37˚C in 5% CO 2 . Cells were placed in airtight chambers (Nu Aire, Plymouth, MN, USA). At the beginning of each experiment, cells were re-suspended in the medium at a density of 2.5×10 5 cells/ml. Cells were treated with 200 mM MSM.
Cell culture and treatment. The gingival cancer cell line YD-38 was maintained in RPMI-1640 containing 10% FBS and 1% penicillin/streptavidin at 37˚C in 5% CO 2 . Cells were placed in airtight chambers (Nu Aire, Plymouth, MN, USA). At the beginning of each experiment, cells were re-suspended in the medium at a density of 2.5×10 5 cells/ml. Cells were treated with 200 mM MSM.
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