Tissue arrayer

Manufactured by Beecher Instruments

Sourced in United States, Moldova, Republic of

The Tissue Arrayer is a laboratory instrument designed for the construction of tissue microarrays (TMAs). It allows for the precise extraction of small tissue core samples from donor tissue blocks and the subsequent placement of these cores into a recipient paraffin block. This process enables the efficient analysis of multiple tissue samples on a single microscope slide.

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Lab products found in correlation

Ultraview universal dab detection kit, by Roche (2 mentions) 3 aminopropyltriethoxysilane, by Merck Group (2 mentions) Bond max autostainer, by Leica (2 mentions) Positively charged slides, by Thermo Fisher Scientific (1 mentions) Superfrost glass slide, by Thermo Fisher Scientific (1 mentions) Hm355s microtome, by Thermo Fisher Scientific (1 mentions) Ab64077, by Abcam (1 mentions) Allprep dna rna ffpe kit, by Qiagen (1 mentions) Qiacube, by Qiagen (1 mentions) Anti phosho akt ser473, by Cell Signaling Technology (1 mentions) Anti p27, by BD (1 mentions) Envision system, by Agilent Technologies (1 mentions) Sc 130164, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Tissue arrayer instrument Programmed tissue arrayer Semi-automated tissue arrayer Precision tissue arrayer Beecher Mark II Tissue Arrayer Tissue Arrayer 1 Beecher Manual Tissue Arrayer Automated tissue arrayer MTA-1 Manual Tissue Arrayer Beecher Tissue Arrayer

41 protocols using tissue arrayer

Immunohistochemical Analysis of hMAGEA2 in Breast Cancer

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The tissue microarrays (TMAs) were constructed with 1 mm diameter core punched two to three distinct regions from each formalin-fixed paraffin-embedded (FFPE) tumor block. The TMAs were assembled with a tissue arrayer (Beecher Instruments, Silver Spring, MD). One section from each TMA was stained with hematoxylin and eosin (H&E) stain and reviewed to confirm the presence of representative tumors. After deparaffinization, rehydration, and antigen retrieval, the localization of hMAGEA2 in TMAs was assessed by immunohistochemistry (IHC) using antibodies (sc-130164, Santa Cruz) and the Ultraview Universal DAB Detection Kit (Ventana, Tucson, AZ). All sections were counterstained with hematoxylin. TMA sections were assessed for the intensity of the stain and the actual percentage of stained cells in the nucleus, cytoplasm, and cell membrane. Staining was considered positive when there was moderate or strong immune reactivity at the appropriate location over the cut-off point. A sample of 25 patients with breast cancer was used and 5 patients with TNBC prognosis.

Park S., Sung Y., Jeong J., Choi M., Lee J., Kwon W., Jang S., Park S.J., Kim H.S., Lee M.H., Kim D.J., Liu K., Kim S.H., Dong Z., Ryoo Z.Y, & Kim M.O. (2017). hMAGEA2 promotes progression of breast cancer by regulating Akt and Erk1/2 pathways. Oncotarget, 8(23), 37115-37127.

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Tissue Microarray Construction Protocol

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The TMA was constructed as previously described (88 (link)). Briefly, FFPE tumour tissue blocks were first cut into sections and stained with haematoxylin and eosin (H&E) to confirm the presence of tumour. An H&E section from each block was marked by a neuropathologist to indicate the most representative tumour area for each patient. Tissue cores of 0.6 mm diameter were punched from the donor marked tumour tissue block using a manual Tissue Arrayer (Beecher Instruments Tissue Arrayer) and inserted into one empty recipient block. The recipient TMA block was cut into 5 μm thick using bench mounted microtome (Thermo Fisher Scientific) and mounted onto positively charged slides (Thermo Fisher Scientific) to maximize tissue adherence.

Al Shboul S., Curran O.E., Alfaro J.A., Lickiss F., Nita E., Kowalski J., Naji F., Nenutil R., Ball K.L., Krejcir R., Vojtesek B., Hupp T.R, & Brennan P.M. (2021). Kinomics platform using GBM tissue identifies BTK as being associated with higher patient survival. Life Science Alliance, 4(12), e202101054.

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Bladder Tumor Tissue Microarray Analysis

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The expression levels of selected genes were tested on parallel tissue microarrays (TMA) comprising the same cohort of 89 conventional and 43 micropapillary FFPE bladder tumor samples from which cDNA microarray was prepared. The tissue microarrays were designed and prepared as previously described[18 (link)]. In brief, tissue microarrays were constructed with a tissue arrayer (Beecher Instruments, Silver Spring, MD). Immunohistochemical staining was performed with mouse monoclonal antibodies against human CD44 (H-CAM DF1485 clone, 1:75 dilution; Leica Biosystems, Buffalo Grove, IL), KRT14 ( LL002 clone, 1:50 dilution; BioGenex, Fremont, CA), GATA3 (HG3-31 clone, 1:100 dilution; Santa Cruz Biotechnology Inc., Santa Cruz, CA) and human uroplakin 2 (BC21 clone, 1:100 dilution; Biocare Medical, Concord, CA). Immunohistochemical stains were performed using the Bond-Max Autostainer (Leica Biosystems, Buffalo Grove, IL). The slides were incubated with the primary antibodies, followed by the visualization reagent linked to a dextran polymer backbone with DAB (3, 3-diaminobenzidine) as a chromogen solution. The slides were counterstained with Mayer’s hematoxylin. Staining was scored manually by two pathologists.

Guo C.C., Dadhania V., Zhang L., Majewski T., Bondaruk J., Sykulski M., Wronowska W., Gambin A., Wang Y., Zhang S., Fuentes-Mattei E., Kamat A.M., Dinney C., Siefker-Radtke A., Choi W., Baggerly K.A., McConkey D., Weinstein J, & Czerniak B. (2016). Gene Expression Profile of the Clinically Aggressive Micropapillary Variant of Bladder Cancer. European urology, 70(4), 611-620.

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Prostate Cancer Tissue Microarray

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Human tissue microarrays were constructed using the Beecher Instruments Tissue-Arrayer™ (Beecher Instruments); the first consisting of 27 benign, 25 Gleason pattern 3 prostate carcinoma, and 15 Gleason pattern 4 prostate carcinoma. The second consisted of two replicate 1 mm cores of 65 visceral metastases (35-lymph node; 18-liver; 6-lung; 2-adrenal; 1-brain; 1-periaortic mass) and 120 bone metastases from 42 patients with CRPC as described previously [49 (link)]. Thirty-one of the 42 patients had matched bone and visceral metastases, 4 had only visceral metastases represented, and 7 had only bone metastases represented.

Haider M., Zhang X., Coleman I., Ericson N., True L.D., Lam H.M., Brown L.G., Ketchanji M., Nghiem B., Lakely B., Coleman R., Montgomery B., Lange P.H., Roudier M., Higano C.S., Bielas J.H., Nelson P.S., Vessella R.L, & Morrissey C. (2015). Epithelial mesenchymal-like transition occurs in a subset of cells in castration resistant prostate cancer bone metastases. Clinical & experimental metastasis, 33(3), 239-248.

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Tissue Microarray Construction for Tumor Analysis

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Cellular areas of the tumors on H&E slides were chosen and the corresponding areas were taken from the paraffin blocks for tissue microarray (TMA) construction. Two 0.6 mm tissue cores were obtained from each case. One additional core was taken from available nodal metastases. The TMAs were assembled with a tissue arrayer (Beecher Instruments, Silver Springs, MD). Sixty two composite TMA blocks, each containing maximum 54 tissue cores, were constructed. Serial 4 micron sections were cut and transferred to Superfrost Plus glass slides (Menzel-Glaser, Germany). One section from each tissue array block was stained with H&E to confirm the presence of representative tumors in the TMA blocks.

Liu Y.H., Tsang J.Y., Ni Y.B., Hlaing T., Chan S.K., Chan K.F., Ko C.W., Mujtaba S.S, & Tse G.M. (2015). Doublecortin-like kinase 1 expression associates with breast cancer with neuroendocrine differentiation. Oncotarget, 7(2), 1464-1476.

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Prostate Cancer Tissue Microarray Construction

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Human tissue microarrays were constructed consisting of 65 soft tissue metastases and 120 bone metastases from 42 patients with advanced prostate cancer as previously described [24 (link)]. This cohort had been recruited and work performed prior to the advent of novel anti-androgens (such as enzalutamide and abiraterone), however 50% of the cohort received radiotherapy and over 95% had received various combinations of therapies (chemotherapeutic agents/ immunotherapies). Samples were obtained from patients who died of mCRPC and who signed written informed consent for a rapid autopsy to be performed ideally within 2 h of death, under the aegis of the Prostate Cancer Donor Program at the University of Washington [24 (link)]. Cohort characteristics are outlined in Additional file 1: Table S1. Two replicate 1 mm cores of soft tissue metastases and bone metastases were taken from every patient where available [25 (link)]. The tissue microarrays were assembled using the Beecher Instruments Tissue-Arrayer™ (Beecher Instruments, Silver Spring, MD).

Lundon D.J., Boland A., Prencipe M., Hurley G., O’Neill A., Kay E., Aherne S.T., Doolan P., Madden S.F., Clynes M., Morrissey C., Fitzpatrick J.M, & Watson R.W. (2017). The prognostic utility of the transcription factor SRF in docetaxel-resistant prostate cancer: in-vitro discovery and in-vivo validation. BMC Cancer, 17, 163.

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Tissue microarray construction protocol

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Tissues embedded in paraffin were sectioned and stained with hematoxylin and eosin (HE), followed by diagnosis by two pathologists. A site with typical pathology was identified in the corresponding paraffin block and marked. The acceptor paraffin block was perforated with a tissue arrayer (Beecher Instruments Inc, Sun Prairie, WI, USA), and a paraffin-embedded tissue core with a diameter of 1.5 mm was taken from the sampling site of the donor paraffin block and implanted into the corresponding hole in the acceptor paraffin block. The tissue array paraffin block was generated according to a predesigned array diagram. A conventional method was used to cut the array into 4-µm-thick continuous sections, which were attached to anti-dewaxing slides and stored at room temperature.

Zhang X., Ji J., Zhang G., Fang C., Jiang F., Ma S, & Hou J. (2017). Expression and significance of B7-H3 and Tie-2 in the tumor vasculature of clear cell renal carcinoma. OncoTargets and therapy, 10, 5417-5424.

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Prostate Cancer Tissue Microarray

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All samples used in the construction of the TMA were used only for men who signed an IRB-approved Informed Consent for use of their tissues samples. A tissue arrayer (Beecher Instruments, Sun Prairie, WI) was used to construct a prostate cancer tissue microarray (Stanford-TMA) comprising an independent set of 234 formalin-fixed, paraffin-embedded primary prostate tumor cases selected from radical prostatectomy specimens collected at Stanford University, with institutional review board approval. Duplicate 0.6 mm tumor cores represented each case, and the series was associated with a minimum clinical follow-up of 5 years and a median follow-up of 8 years.

Sahoo D., Wei W., Auman H., Hurtado-Coll A., Carroll P.R., Fazli L., Gleave M.E., Lin D.W., Nelson P.S., Simko J., Thompson I.M., Leach R.J., Troyer D.A., True L.D., McKenney J.K., Feng Z, & Brooks J.D. (2018). Boolean analysis identifies CD38 as a biomarker of aggressive localized prostate cancer. Oncotarget, 9(5), 6550-6561.

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DLBCL Tissue Microarray Construction

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Archived tumor specimens were retrieved and hematoxylin and eosin stained (H&E) slides were reviewed to confirm the DLBCL diagnosis as well as to identify representative tumor blocks for tissue microarray (TMA) construction (at the UCLA Core Microarray Facility). Whenever possible three 0.6-mm cores from different areas of the donor block were obtained from each case and inserted in a grid pattern into a recipient paraffin block using a tissue arrayer (Beecher Instruments, Silver Spring, MD).

CHAO C., XU L., SILVERBERG M.J., MARTÍNEZ-MAZA O., CHEN L.H., CASTOR B., ABRAMS D.I., ZHA H.D., HAQUE R, & SAID J. (2015). Stromal immune infiltration in HIV-related diffuse large B-cell lymphoma is associated with HIV disease history and patient survival. AIDS (London, England), 29(15), 1943-1951.

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Tissue Microarray Construction and Analysis

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TMA blocks were built using Tissue-arrayer^® (Beecher Instruments Inc., Sun Prairie, WI, USA). For each case, six tumor cores (0.6-mm diameter) of tumor were transferred from the selected tumor areas to the recipient block. Sections of 5 µm were cut on a microtome and transferred to Superfrost^™ glass slides (Thermo Fisher Scientific, Inc., Waltham, MA, USA). The first slides were colored routinely to attest the presence of tumor cells on a spot. For each tumor included, only the most tumor cell-rich and best conserved spot was considered for further FISH analyses. In this manner, the potential impact of likely intra-tumor heterogeneity was minimized by analyzing a single and identical tissue area per TMA-included tumor with the two commercial and bacterial artificial chromosome (BAC) probes FISH assays.

Uguen A., Uguen M., Talagas M., Gobin E., Marcorelles P, & De Braekeleer M. (2016). Fluorescence in situ hybridization testing of chromosomes 6, 8, 9 and 11 in melanocytic tumors is difficult to automate and reveals tumor heterogeneity in melanomas. Oncology Letters, 12(4), 2734-2741.

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