Gapdh cat no 10494 1 ap

The normal human liver cell line (L02) was purchased from The Cell Collection Center of Wuhan University. The Cell Counting Kit-8 (CCK-8) assay was purchased from Dojindo Chemical Technology Co., Ltd. GLY was purchased from Selleck Chemicals. The lactate dehydrogenase (LDH), malondialdehyde (MDA), glutathione (GSH) and ROS kits were purchased from Beyotime Institute of Biotechnology. The primary antibodies against glutathione peroxidase 4 (GPX4, cat. no. sc-166570) was purchased from Santa Cruz Biotechnology, Inc. GAPDH (cat. no. 10494-1-AP) were purchased from ProteinTech Group, Inc. The primary antibodies against high mobility group box 1 (HMGB1, cat. no. 3935), nuclear factor E2-related factor 2 (Nrf2, cat. no. 12721) and homooxygenase-1 (HO-1, cat. no. 43966) were purchased from Cell Signaling Technology, Inc. The goat anti-rabbit IRDye fluorescent secondary antibody IRDye800CW (cat. no. 926-32211) was purchased from LI-COR Biosciences. Cy3 (cat. no. BA1031 and BA1032) and FITC (cat. no. BA1101 and BA1105) fluorescently labeled rabbit anti-goat secondary antibodies were purchased from Wuhan Boster Biological Technology, Ltd. The iron ion detection kit was purchased from Abcam. D-GalN, LPS and TNF-α were purchased from Sigma-Aldrich; Merck KGaA.

SAL, Sirtinol and 3-(4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent were purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany). Hoechst 33342 solution, the BCA protein assay kit, radioimmunoprecipitation (RIPA) lysis buffer and BeyoECL Plus were purchased from Beyotime Institute of Biotechnology (Shanghai, China). The caspase-3 enzyme-linked immunosorbent assay (ELISA) kit was purchased from the Nanjing Jiancheng Bioengineering Research Institute (cat no. H076; Nanjing, China). The Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining kit was obtained from Sigma-Aldrich; Merck KGaA. Antibodies against SIRT1 (cat no. 9475), phosphorylated (p)-FOXO3α (cat no. 9466), B-cell lymphoma 2 (Bcl-2; cat no. 3498) and Bcl-2 associated X protein (Bax; cat no. 2772) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). GAPDH (cat no. 10494-1-AP) and secondary antibodies were obtained from ProteinTech Group, Inc. (Chicago, IL, USA).