Total RNA was isolated with Isogen reagent (NIPPON GENE CO., LTD., Tokyo, Japan) and purified with RNAeasy mini kit (QIAGEN, California, USA) according to the manufacturer's instructions. Total RNA concentration and purity were checked with NanoDrop spectrophotometer (NanoDrop Technologies Inc., Delaware, USA). The quality of total RNA was assessed by electrophoretic separation on an RNA Nano lab chip, using a 2100 Bioanalyzer (Agilent Technologies Inc., California, USA). The total RNA samples were amplified and labeled with Cy3 by using the Quick Amp labeling Kit and hybridized with an Agilent 4×44 K Whole Mouse Genome Microarray (Agilent Technologies Inc.). Then, the array was scanned with Dual-Laser microarray Scanner G2565AA (Agilent Technologies Inc.). The scanned data were analyzed using Feature Extraction Software 9.1 (Agilent Technologies Inc.), which tagged the data as signals recognized as being outliers or equal to the background. The fold change of each gene was calculated as the ratio of signal intensity between the experimental average data and the control average data.
Agilent 4 44 k whole mouse genome microarray
Manufactured by Agilent Technologies
The Agilent 4x44K Whole Mouse Genome Microarray is a high-density microarray platform that enables the analysis of the expression of approximately 45,000 mouse genes and transcripts. It provides a comprehensive view of the mouse transcriptome, allowing researchers to study gene expression patterns and profiles in mouse models.
Automatically generated - may contain errors
Lab products found in correlation
Feature extraction software 9, by Agilent Technologies (1 mentions)
Isogen reagent, by Nippon Gene (1 mentions)
Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions)
2100 bioanalyzer, by Agilent Technologies (1 mentions)
Rnaeasy mini kit, by Qiagen (1 mentions)
Sybr green jumpstart taq readymix, by Merck Group (1 mentions)
Agilent microarray scanner, by Agilent Technologies (1 mentions)
Revertaid m mulv reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Rabbit anti mouse igg peroxidase conjugate, by Merck Group (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Faststart universal sybr green master rox, by Roche (1 mentions)
3 protocols using agilent 4 44 k whole mouse genome microarray
1
Whole Mouse Genome Microarray Analysis
2
Pancreatic Islet RNA Extraction and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from pancreatic islets isolated by the standard collagenase P (Roche, 11 213 873 001) protocol (31 (link)) or whole pancreata. Total RNA preparation and cDNA synthesis were performed as described (23 (link)). Quantitative PCR (qPCR) reactions were performed using SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich, S4438) and a Stratagene Mx3005P qPCR system. Fold enrichment of mRNA message was calculated by normalizing to a reference gene (see Supplementary Table 3 for qPCR primers). Control and mutant-isolated islet total RNA extractions were matched for pancreatic endocrine purity as described (32 (link)). Microarray analysis was performed by the University of Pennsylvania’s Diabetes Research Center Functional Genomics Core. RNA was labeled with the Agilent Low Input Kit and hybridized, using a dye-switch design, to the Agilent 4 × 44K Whole Mouse Genome Microarray. Arrays were hybridized overnight and scanned using the Agilent Microarray Scanner. Data were normalized using normalizeBetweenArrays from the Limma package followed by SAMR to identify differentially expressed genes.
3
Influenza Vaccine Preparation and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Newcastle disease virus (NDV)-based recombinant influenza vaccine (rL-H5) and H5 subtype AIV hemagglutination inhibition detecting antigen (H5Ag) were purchased from the Harbin Weike Biotechnology Development Co., Heilongjiang, China. RPMI medium was from Hyclone/GE Healthcare, Logan, UT, USA; fetal bovine serum (FBS) was from Gibco, Grand Island, NY, USA. Rabbit anti-mouse IgG peroxidase conjugate were purchased from Sigma Chemical Co., St. Louis, MO, USA; goat anti-mouse IgG1 and IgG2b peroxidase conjugates were from Southern Biotech. Assoc., Birmingham, AL, USA; goat anti-mouse IgG2a peroxidase conjugates were from Abcam, Cambridge, UK. Trizol reagent was purchased from Invitrogen, Carlsbad, CA, USA; revert Aid™ M-MuLV reverse transcriptase was from Fermentas, USA; diethylpyrocarbonate (DEPC), ribonuclease inhibitor, and oligo(dT)18 were from Shanghai Sangon Biological Engineering Technology Co., Ltd., Shanghai, China; FastStart Universal SYBR Green Master (ROX) was from Roche Diagnostics Ltd., Shanghai, China. Agilent 4 × 44 k whole mouse genome microarray was provided from Agilent Technologies. Santa Clara, CA, USA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!