Human recombinant interferon beta ifnβ

Human recombinant interferon beta (IFNβ) was obtained from R&D System (NY, USA). Soluble, recombinant human TRAIL, Super FAS Ligand, the primary mouse monoclonal antibodies against TRAIL, TRAIL-R1 and TRAIL-R2 were purchased from Enzo Life Science (Paris, France). The caspase inhibitors Z-VAD-fmk, Z-IETD-fmk and Z-LEHD-fmk were obtained from R&D System (Wiesbaden, Germany). Antibodies used for immunoblotting were mouse anti-human β-actin (Cell Signaling, Danvers, MA, USA), mouse anti-human-TRAIL (Enzo Life Science), mouse anti-human caspase-3 (Cell Signaling), and mouse anti-human caspase-8 (Enzo Life Science). The goat anti-mouse IgG secondary antibody was purchased from Santa Cruz Biotechnology (Heidelberg, Germany). The FITC-active caspase-3 apoptosis test was obtained from BD Pharmingen (San Diego, CA, USA). Hoechst 33258 was purchased from Sigma (St. Louis, MO, USA), Rotitest Vital from Roth (Karlsruhe, Germany). Knock-down experiments for TRAIL-RNA were performed using TRAIL-siRNA (Dharmacon, Freiburg, Germany, Cat. Nr. L-011524-00-0010) and scrambled RNA (Dharmacon, Cat. Nr. D-001810-20).