Anti cyp8b1

Proteins were extracted from liver tissue using RIPA lysis buffer (Beyotime Institute of Biotechnology, Shanghai, China), containing a protease and phosphatase inhibitor cocktail (Beyotime Institute of Biotechnology). Equal amounts of extracted proteins were separated by electrophoresis on 7.5% or 10% SDS-polyacrylamide gels and electrotransferred onto polyvinylidene fluoride membranes (Millipore, Tullagreen, Ireland). The membranes were blocked, incubated with appropriate primary antibodies at 4°C overnight, and subsequently incubated with secondary antibody (catalog number, 7074S, 7076S; Cell Signaling Technology, Danvers, MA, USA). Bands were detected using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) with a ChemiDoc MP imaging system (Bio-Rad Laboratories, Hercules, CA, USA).
The antibodies used, all of which were generated in rabbits and diluted 1:1000, were: anti-Gapdh (catalog number, 5174S; Cell Signaling Technology), anti-Abcg8 (DF6673; Affinity, Changzhou, China), anti-Cyp8b1 (ab191910; Abcam, Cambridge, UK), anti-Abcg5 (27722-1-AP; Proteintech, Rosemont, IL, USA), and anti-Cyp7b1 (24889-1-AP; Proteintech). Mouse anti-Cyp7a1 antibody (sc-518007; Santa Cruz Biotechnology, Dallas, TX, USA) was diluted 1:1500.

The following antibodies were used for immunofluorescence staining or western blot analyses: anti-FoxA2 (used at 1:400, Cell Signaling), anti-HNF4α (used at 1:500, Cell Signaling), anti-AFP (used at 1:1000, Sigma-Aldrich), anti-ALB (used at 1:1000, Cedarlane, Burlington, Canada), anti-ZO-1 (used at 1:1000, Thermo Fisher), anti-E cadherin (used at 1:500, Cell Signaling), anti-SR-BI (used at 1:100, Novus Biologicals), anti-BCRP (used at 1:100, Millipore), anti-MRP2 EAG5^{48 (link)} (used at 1:200, a kind gift from Anne Nies, IKP Stuttgart^{48 (link)}), anti-Apo-CIII (used at 1:500, Abcam), anti-CYP8B1 (used at 1:100, Abcam), anti-ORF2 (used at 1:400, a kind gift from Suzanne U. Emerson, NIH) and anti-HAV capsid (used at 1:1000, a kind gift from Stanley M. Lemon, UNC School of Medicine). Alexa Fluor 488 and 549 anti-mouse (used at 1:1000) and Alexa Fluor 488 and 549 anti-rabbit (used at 1:1000) antibodies were purchased from ThermoFisher. Alexa 594-conjugated transferrin was purchased from ThermoFisher. Tenofovir, Tenofovir disoproxil fumarate, and Emtricitabine were obtained through the AIDS Reagent Program, Division of AIDS, NIAID, NIH. Elvitegravir and Cobicistat were purchased from SelleckChem. Sofosbuvir was purchased from Acme Bioscience. BX795, oleic acid and lomitapide were purchased from Sigma-Aldrich.