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Ab204537

Manufactured by Abcam
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Sourced in United Kingdom, United States

Ab204537 is a laboratory equipment product. It is a general-purpose analytical tool used for various scientific applications.

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Lab products found in correlation

Ab83362, by Abcam (3 mentions) Creatinine assay kit, by Abcam (1 mentions) Cat no ab46070, by Abcam (1 mentions) Cat no elr il6 001, by RayBiotech (1 mentions) Glutathione gsh assay kit, by Cayman Chemical (1 mentions)

5 protocols using ab204537

1

Amino Acid and Creatinine Quantification

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Amino acids were determined as previously described by Weger et al. [41 (link)]. The panel of 17 amino acids included alanine (Ala), arginine (Arg), asparagine (Asn), aspartate (Asp), glutamine (Gln), glutamate (Glu), glycine (Gly), histidine (His), Isoleucine (Ile), leucine (Leu), lysine (Lys), methionine (Met), phenylalanine (Phe), proline (Pro), serine (Ser), tyrosine (Tyr) and valine (Val). Creatinine determination in murine urine samples was performed with the creatinine assay kit (Abcam, ab204537) following precisely the manufacturer’s protocol.
Heidenreich E., Pfeffer T., Kracke T., Mechtel N., Nawroth P., Hoffmann G.F., Schmitt C.P., Hell R., Poschet G, & Peters V. (2021). A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles. International Journal of Molecular Sciences, 22(18), 9979.
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2

Serum Creatinine and Urea Measurement

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According to the commercially available kits, serum creatinine and urea were assessed in accordance with their manufacturer’s instructions (ab204537 and ab83362, respectively; Abcam, Cambridge, UK).
Antar S.A., Abd-Elsalam M., Abdo W., Abdeen A., Abdo M., Fericean L., Raslan N.A., Ibrahim S.F., Sharif A.F., Elalfy A., Nasr H.E., Zaid A.B., Atia R., Atwa A.M., Gebba M.A, & Alzokaky A.A. (2023). Modulatory Role of Autophagy in Metformin Therapeutic Activity toward Doxorubicin-Induced Nephrotoxicity. Toxics, 11(3), 273.
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3

Creatinine Assay Protocol

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Plasma creatinine was measured with the fluorometric Creatinine assay kit (ab65340, Abcam), and urine creatinine with the colorimetric Creatinine assay kit (ab204537, Abcam).
De Castro L., Manoury A., Claude O., Simoneau B., Monceau V., Suhard D., Elie C., Magneron V., Roy L., Bouvier-Capely C., Ibanez C., Davesne E, & Guéguen Y. (2023). Renal toxicity and biokinetics models after repeated uranium instillation. Scientific Reports, 13, 4111.
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4

Biochemical Assays for Clinical Analysis

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Urea assay kit (abcam, ab83362) was measured according to the method described by Salama et al. (2013) (link), creatinine Assay kit (abcam, ab204537) measured according to the method of Granger et al. (1996) (link) and electrolytes according to Jain et al. (2009) (link).
Morsi R.M., Mansour D.S, & Mousa A.M. (2022). Ameliorative potential role of Rosmarinus officinalis extract on toxicity induced by etoposide in male albino rats. Brazilian journal of biology = Revista brasleira de biologia, 84.
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5

Quercetin Antioxidant and Inflammatory Assays

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Reagents and assay kits. Quercetin (C15H10O7, CAS Number 117-39-5) was purchased from Sigma-Aldrich (St. Louis, MO, USA) and was prepared daily and freshly by dissolving it in a normal saline solution (0.9 % NaCl) to the final concentration of 50mg/ml. Assay kits for determination of malondialdehyde (MDA, Cat No. NWK-MDA01) were purchased from NWLSS (Vancouver, BC, Canada). Glutathione (GSH) assay kit was purchased from Cayman Chemical, Cat. No.703002, MI, USA. ELISA kits for determination the levels of IL-6 (Cat No. ELR-IL6-001) was purchased from RayBio, GA, USA. ELISA kits for determination of TNF-a (Cat No. ab46070) was purchased from Abcam, Cambridge, UK. Urea and creatinine were determined by colorimetric assay using assay kits (ab83362 and ab204537) from Abcam, MA, USA.
Bayoumy N. (2020). Quercetin Protects Against Acetaminophen-Induced Acute Nephrotoxicity Associated with the Inhibition of Biomarkers of Acute Kidney Injury in Rats. International Journal of Morphology, 38(4), 876-881.
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