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Anti ha 3f10

Manufactured by Santa Cruz Biotechnology
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Anti-HA 3F10 is a monoclonal antibody that specifically recognizes the hemagglutinin (HA) epitope tag. It is a useful tool for the detection and purification of HA-tagged proteins in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Histone 3, by Merck Group (2 mentions) Odyssey imaging system, by LI COR (2 mentions) Irdye 800cw, by LI COR (2 mentions) Anti tubulin, by Santa Cruz Biotechnology (2 mentions)

2 protocols using anti ha 3f10

1

Western Blot Analysis of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were separated on 12% SDS-polyacrylamide gels and transferred to PVDF membranes. Membranes were incubated with primary antibodies: anti-HA 3F10 (Rat, 1:2500), anti-Tubulin (polyclonal rabbit anti β Tubulin H-235 from Santa Cruz Biotechnology, 1:2500) and Histone-3 (polyclonal rabbit anti H3 H-0164 from Merk, 1:2500). After washing, membranes were incubated with IRDye® 800CW (LI-COR Biosciences) secondary antibodies. Bound antibody was detected an Odyssey imaging system (LI-COR Biosciences).
Fuchs Wightman F., Lukin J., Giusti S.A., Soutschek M., Bragado L., Pozzi B., Pierelli M.L., González P., Fededa J.P., Schratt G., Fujiwara R., Wilusz J.E., Refojo D, & de la Mata M. (2024). Influence of RNA circularity on Target RNA-Directed MicroRNA Degradation. Nucleic Acids Research, 52(6), 3358-3374.
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2

Western Blot Analysis of Protein Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein samples were separated on 12% SDS-polyacrylamide gels and transferred to PVDF membranes.
Membranes were incubated with primary antibodies: anti-HA 3F10 (Rat, 1:2500), anti-Tubulin (polyclonal rabbit anti βtubulin H-235 from Santa Cruz Biotechnology, 1:2500) and Histone-3 (polyclonal rabbit anti H3 H-0164 from Merk, 1:2500). After washing, membranes were incubated with IRDye® 800CW (LI-COR Biosciences) secondary antibodies. Bound antibody was detected an Odyssey imaging system (LI-COR Biosciences).
Wightman F.F., Lukin J., Giusti S.A., Soutschek M., Bragado L., Pozzi B., González P., Fededa J.P., Schratt G., Fujiwara R., Wilusz J.E., Refojo D., & Mata M.d.l. (2022). Influence of RNA circularity on Target RNA-Directed MicroRNA Degradation.
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