After the collection of cardiac and peripheral blood samples, the blood serum and red blood cells were rapidly and carefully separated by centrifugation at 1000×g for 10 min. The concentrations of tumor necrosis factor (TNF)-α (Abbkine, Wuhan, China), interleukin (IL)-1β (Abbkine), IL-6 (Abbkine), cardiac troponin I (cTnI) (Nanjing Jiancheng BioEngineering Institute, Nanjing, China), and brain natriuretic peptide (BNP) (Nanjing Jiancheng BioEngineering Institute) in the peripheral blood were determined using commercially available ELISA kits according to the manufacturer’s instructions.
Il 1β
Manufactured by Abbkine
Sourced in China
IL-1β is a pro-inflammatory cytokine that plays a crucial role in the immune response. It is involved in a variety of cellular activities, including the stimulation of the acute phase response. IL-1β is commonly used in research applications to study inflammation and immune system function.
Automatically generated - may contain errors
Lab products found in correlation
Tnf α, by Abbkine (2 mentions)
Interleukin 6 (il 6), by Abbkine (1 mentions)
Drp300, by R&D Systems (1 mentions)
Adi 900 071, by Enzo Life Sciences (1 mentions)
Victor x4 multimode plate reader, by PerkinElmer (1 mentions)
Ket6017, by Abbkine (1 mentions)
Ket6013, by Abbkine (1 mentions)
Dcrp00, by R&D Systems (1 mentions)
Pinaverium bromide, by Abbott (1 mentions)
Indomethacin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 protocols using il 1β
1
Quantifying Inflammatory Markers and Cardiac Biomarkers
2
Quantification of Inflammatory Biomarkers
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The levels of inflammatory cytokines and chemokines were established in plasma samples and the levels of cortisol and adiponectin were established in serum samples. Undiluted samples were used for inflammatory cytokines and chemokines, and samples for CRP and adiponectin were diluted 200-fold. All biomarkers were analyzed in duplicate using the following enzyme-linked immunosorbent assay (ELISA) kits: IL-1β (KET6013; Abbkine, Wuhan, China), IL-6 (KET6017; Abbkine, Wuhan, China), TNF-α (ADI-900-099; Enzo, Madison Avenue, New York, NY, USA), IL-17 (KET6022; Abbkine, Wuhan, China), CRP (DCRP00; R&D Systems, Minneapolis, MN, USA), adiponectin (DRP300; R&D Systems, Minneapolis, MN, USA), cortisol (ADI-900-071; Enzo, New York, NY, USA), CCL1 (MBS824930; MyBioSource, San Diego, CA, USA), and CCL2 (LS-F146; LSBio, Seattle, WA, USA), according to the manufacturer’s instructions. The standard solutions were respectively prepared using the reagents provided in each kit. Plates were read at 450 nm using a VICTOR X4 Multimode Plate Reader (PerkinElmer, Waltham, MA, USA). Protein quantification was performed using the mean value of the duplicate samples.
3
Investigating Gastrointestinal Inflammation
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The valerian extract capsule was obtained from Wuhan United Pharmaceutical Co. Ltd. (Hubei, China). Teprenone was purchased from Eisai China Inc. (Jiangsu, China). Indomethacin was acquired from Sigma (St. Louis, MO, USA). Pinaveriumbromide was purchased from Abbott (Chicago, IL, USA). Atropine was purchased from Tianjin Kingyork Pharmaceuticals Co., Ltd. (Tianjin, China). Neostigmine methylsulfate injection was purchased from Shanghai Sine Jinzhu Pharmaceutical Co., Ltd. (Shanghai, China). The enzyme-linked immunosorbent assay (ELISA) kits measured MPO, MDA, SOD, GSH-PX, PAF, IL-1β, TNF-α, PGE2, PGI2, and COX-1 were purchased from Abbkine Scientific Co. Ltd. (Santa Cruz, CA, USA).
4
In Vitro Chondrocyte Inflammation Model
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The ADTC5 cells (HTX2518, OTWO, Shenzhen, China) were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12) (L320KJ, BasalMedia, Shanghai, China) supplemented with 10% fetal bovine serum (10,270,106, Gibco, CA, USA) and 1% Penicillin–Streptomycin Solution 100 × (SL6040, Coolaber, Beijing, China) at 37 ◦C in 5% CO2 (#3111, Thermo Fisher Scientific, MA, USA).
Cells contain 6 groups: normal control (CON) group, OA model (MOL) group, solvent control (DMSO) group and different dose of calycosin (CAL) groups. In order to simulate the pathological process of aseptic inflammation in chondrocytes ADTC5 cells in vitro, we used IL-1β (prp1119, Abbkine Scientific, Wuhan, China)10 ng/mL as a stimulation factor [64 (link)]. The cells were pretreated with different concentrations (0, 16, 32, and 64 µM) of calycosin (A0514, MUST Biotechnology, Chengdu, China) with 10 ng/mL IL-1β for 24 h. DMSO (CD4731C, Coolaber) control was performed at the same concentrations with 32 µM.
Cells contain 6 groups: normal control (CON) group, OA model (MOL) group, solvent control (DMSO) group and different dose of calycosin (CAL) groups. In order to simulate the pathological process of aseptic inflammation in chondrocytes ADTC5 cells in vitro, we used IL-1β (prp1119, Abbkine Scientific, Wuhan, China)10 ng/mL as a stimulation factor [64 (link)]. The cells were pretreated with different concentrations (0, 16, 32, and 64 µM) of calycosin (A0514, MUST Biotechnology, Chengdu, China) with 10 ng/mL IL-1β for 24 h. DMSO (CD4731C, Coolaber) control was performed at the same concentrations with 32 µM.
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