Rabbit anti hdac6

Manufactured by Santa Cruz Biotechnology

Sourced in United Kingdom

Rabbit anti-HDAC6 is a primary antibody that recognizes the histone deacetylase 6 (HDAC6) protein. HDAC6 is a class II histone deacetylase enzyme that plays a role in the regulation of various cellular processes, including microtubule dynamics, protein trafficking, and stress response.

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Lab products found in correlation

Close spelling variants of this product

HDAC6 (16 citations) Anti-HDAC6 (14 citations) Rabbit anti-TM (2 citations) Rabbit anti-HDAC6 (H-300; sc-11420) (2 citations)

2 protocols using rabbit anti hdac6

Protein Immunoblotting and Immunostaining

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The following reagents were purchased from the indicated providers: dimethyl sulfoxide (DMSO; Sigma, D8418) and mifepristone (RU-486; Sigma, M8046). The following antibodies were used for immunoblotting: mouse anti-TurboGFP (Origene, TA150041), rabbit anti-TDP-43 (Proteintech, 10782-2-AP), rabbit anti-LC3 (MBL, PM036), mouse anti-Polyubiquitin (Enzo Life Science, BML-PW8805), mouse anti-Flag (Cell Signaling Technology, 2044), rabbit anti-HDAC6 (Santa Cruz Biotechnology, sc-11420), mouse anti-Lamin A/C (EMD Millipore, 05-714), HRP-conjugated anti-alpha-tubulin (Cell Signaling Technology, 9099), HRP-conjugated anti-rabbit IgG (Santa Cruz Biotechnology, sc-2004), HRP-conjugated mouse IgM (Abcam, ab97230), and HRP-conjugated mouse IgG (Santa Cruz Biotechnology, sc-2005). The following antibodies were used for immunocytochemistry (ICC): rabbit anti-cleaved caspase-3 (CC3) antibody (Cell Signaling Technology, 9664) and Alexa 594-conjugated anti-rabbit IgG (Jackson ImmunoResearch, 111-585-144). The following antibodies were used for immunohistochemistry: rat anti-ELAV (DSHB, RAT-ELAV-7), mouse anti-Polyubiquitin (Enzo Life Science, BML-PW8805), Alexa-488 conjugated rat IgG (Jackson ImmunoResearch, 112-545-167), and Alexa-594 conjugated mouse IgM (Jackson ImmunoResearch, 115-587-020).

Lee S., Kwon Y., Kim S., Jo M., Jeon Y.M., Cheon M., Lee S., Kim S.R., Kim K, & Kim H.J. (2020). The Role of HDAC6 in TDP-43-Induced Neurotoxicity and UPS Impairment. Frontiers in Cell and Developmental Biology, 8, 581942.

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Comprehensive Western Blot Characterization

Cited 1 time

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Western blot protocols have been previously published [23 (link)]. Immunoprobing was performed using the following antibodies: mouse anti-acetylated α-tubulin (Santa Cruz Biotechnologies, Dallas, TX, USA), rabbit anti-actin (Sigma Aldrich), rabbit anti-Notch3 (Abcam, Cambridge, UK), rabbit anti-Notch1 (Cell Signaling), mouse anti-c-Myb (Thermo Fisher Scientific), rabbit anti-HDAC6 (Santa Cruz Biotechnologies), rabbit anti-Histone H3 (Cell Signaling or Abcam), rabbit anti-acetyl-Histone H3 (Lys 9) (Cell Signaling), mouse anti-LAMP2 (Novus Biologicals, Littleton, CO, USA), mouse anti-Pan-cadherin (Santa Cruz Biotechnologies), mouse anti-GAPDH (Santa Cruz Biotechnologies), rabbit anti-VDAC (Cell Signaling) followed by incubation with a horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary Ab (Perkin Elmer, Waltham, MA, USA). Antigens were identified using Western Lightning plus ECL (Perkin Elmer) or ECL Select Western Blotting detection (GE Health Care) reagents and detected by UVITec Alliance LD2 (UVITec Cambridge, UK) imaging system.

Pinazza M., Ghisi M., Minuzzo S., Agnusdei V., Fossati G., Ciminale V., Pezzè L., Ciribilli Y., Pilotto G., Venturoli C., Amadori A, & Indraccolo S. (2018). Histone deacetylase 6 controls Notch3 trafficking and degradation in T-cell acute lymphoblastic leukemia cells. Oncogene, 37(28), 3839-3851.

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