Log In Sign up
The largest database of trusted experimental protocols

Ifi44

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The IFI44 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a specific function, but without more information, a detailed and unbiased description cannot be provided while maintaining conciseness. A comprehensive and factual description is not available at this time.

Automatically generated - may contain errors

Lab products found in correlation

M mlv reverse transcriptase, by Thermo Fisher Scientific (2 mentions) Il 17a, by Thermo Fisher Scientific (1 mentions) Ifna1, by Thermo Fisher Scientific (1 mentions) Taqman gene expression master mix, by Thermo Fisher Scientific (1 mentions) Ifi16, by Thermo Fisher Scientific (1 mentions) Fx96 cycler, by Bio-Rad (1 mentions) Ifih1, by Thermo Fisher Scientific (1 mentions) Socs1, by Thermo Fisher Scientific (1 mentions) Taqman primer probe set, by Thermo Fisher Scientific (1 mentions) Mgcl2, by Thermo Fisher Scientific (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Tapestation 4200, by Agilent Technologies (1 mentions) Hprt1, by Thermo Fisher Scientific (1 mentions) Polr2a, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions) Lightcycler 480 2, by Roche (1 mentions) Tap63, by Cell Signaling Technology (1 mentions) Hdac3, by Cell Signaling Technology (1 mentions) Nupage tris hcl precast gels, by Thermo Fisher Scientific (1 mentions)

4 protocols using ifi44

1

qPCR Analysis of Immune Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
qPCR was performed using the TaqMan Gene Expression Master Mix (Applied Biosystems, Thermo Fisher Scientific, 4369016). Data were analyzed by the 2-DDCt method using the mean adult values as the reference. Primers/probes were from Thermo Fisher Scientific: MX1 (Hs00895608_m1), MX2 (Hs01550811_m1), IFNA1 (Hs00256882_s1), IFI44 (Hs00951349_m1), IFIT1 (Hs03027069_s1), IL17A (Hs00174383_m1), IFNG (Hs00989291_m1), RPLPO (4326314E).
Pierce C.A., Sy S., Galen B., Goldstein D.Y., Orner E., Keller M.J., Herold K.C, & Herold B.C. (2021). Natural mucosal barriers and COVID-19 in children. JCI Insight, 6(9), e148694.
+ Open protocol
+ Expand
2

Quantifying Immune Response Transcripts in Neutrophils

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using the RNeasy Mini kit following the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA quality and quantification were determined by TapeStation 4200 (Agilent, St. Clara, USA) following the manufacturer’s instructions. Neutrophil total RNA was isolated using RNeasy Mini kit following the manufacturer’s instructions (Qiagen), and complementary DNA (cDNA) was synthesized using M-MLV Reverse Transcriptase (Thermo Fisher Scientific). RT-PCR was performed in duplicates using the cDNA and Platinum Taq polymerase (Thermo Fisher Scientific), 200 nM dNTP (Promega, Southampton, UK), 50mM MgCl2 (Thermo Fisher Scientific), and TaqMan primer/probe sets (Thermo Fisher Scientific). Samples were matched to a standard curve generated by amplifying serially diluted products using the same PCR reaction and normalized to GAPDH (Hs00266705_g1) to obtain the relative expression value. Real-time assays were run on FX96 Cycler (Bio-Rad). The following are the primes used: STAT1 (Hs01013996_m1), STAT2 (Hs01013115_g1), STAT3 (Hs00374280_m1), IFIH1 (Hs00223420_m1), IFIT (Hs00356631_g1), ISG15 (Hs00192713_m1), MX1 (Hs00895608_m1), IRF1 (Hs00971965_m1), SOCS1 (Hs00705164_s1), USP18 (Hs00276441_m1), IFI44 (Hs00197427_m1), IFI16 (Hs00986757_m1), and OAS (Hs00242943_m1) (all from Thermo Fisher Scientific).
Parackova Z., Vrabcova P., Zentsova I., Sediva A, & Bloomfield M. (2023). Neutrophils in STAT1 Gain-Of-Function Have a Pro-inflammatory Signature Which Is Not Rescued by JAK Inhibition. Journal of Clinical Immunology, 43(7), 1640-1659.
+ Open protocol
+ Expand
3

Quantifying Immune Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from cells (1–2 × 106 cells per sample) with an RNeasy kit (Qiagen, Germantown, MD, USA) and cDNA was generated using an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). qRT-PCR was performed on a Light Cycler 480 II (Roche, Indianapolis, IN, USA). Primers for IFIT1 (Hs03027069), IFI44 (Hs00197427), RAGE (Hs00542584), FcRIIa (Hs01013401), MX1 (Hs00182073,), HPRT1 (Hs99999909), and Polr2a (Hs00172187) were purchased from Thermo Scientific. The genes of interest were normalized to the expression of the house keeping gene (Polr2a) and were compared to a control condition with no treatment. The relative induction was calculated by 2−ΔΔCt.
Porat A., Giat E., Kowal C., He M., Son M., Latz E., Ben-Zvi I., Al-Abed Y, & Diamond B. (2018). DNA-Mediated Interferon Signature Induction by SLE Serum Occurs in Monocytes Through Two Pathways: A Mechanism to Inhibit Both Pathways. Frontiers in Immunology, 9, 2824.
+ Open protocol
+ Expand
4

Western Blot Analysis of TNBC PDX Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols
About 50 mg of frozen TNBC PDX tumors were used to extract total protein with T-PER Tissue Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s guidelines. The protein concentration was evaluated by Bradford Assay. The equal amount of denatured protein extracts was separated through electrophoresis in 4–15% NuPAGE Tris-HCl precast gels (Invitrogen, Waltham, MA, USA) and transferred onto nitrocellulose membranes. Membranes were then probed with primary antibodies including Cd74, Sat1, TAp63, Bcl-xL, Dnmt1, Dnmt3a, Dnmt3b, Tet2, Tet3, Hdac1, Hdac2, Hdac3, Hdac8 (Cell Signaling Technology, Danvers, MA, USA), Tet1, Ifi44, Fzd9, Wwc1 (Thermo Fisher Scientific, Waltham, MA, USA) and NF-κB and Lpl (Santa Cruz, Dallas, TX, USA). β-actin served as an internal control for each membrane. Immunoreactive bands were visualized using Clarity MaxTM Western ECL Blotting Substrates (Bio-Rad, Hercules, CA, USA) and images were captured using ChemiDocTM Imaging Systems (Bio-Rad, Hercules, CA, USA). The protein expression levels were quantified using Image J software (v1.53e).
Sharma M., Arora I., Chen M., Wu H., Crowley M.R., Tollefsbol T.O, & Li Y. (2021). Therapeutic Effects of Dietary Soybean Genistein on Triple-Negative Breast Cancer via Regulation of Epigenetic Mechanisms. Nutrients, 13(11), 3944.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!