BEAS-2B cells (ATCC CRL-9609) were cultured from cryopreserved stocks in collagen-coated T-75 culture flasks according to ATCC guidelines. Briefly, cells were seeded at 3,000 cells/cm2 and cultured in 23 mL of bronchial epithelial cell growth medium (BEGM) (Lonza, CC-3170), omitting the addition of the gentamicin–amphotericin aliquot to the medium, as recommended by ATCC. Cells were grown at 37°C in a humidified incubator with a 5% CO2 atmosphere, and complete media exchanges were performed every 48 h. After approximately 4 days, the cultures reached ∼70% confluency, and cells were subcultured into 6-well or 96-well plates coated with type 1 collagen (Advanced BioMatrix, Cat#5005) at 1,500 cells/cm2, and allowed to attach to the growth surface for 24 h before exposure to PM.
Cat 5005
Manufactured by Advanced BioMatrix
Cat#5005 is a laboratory equipment used for [specific core function]. It is designed to [brief description of core function] without any further interpretation or extrapolation on its intended use.
Automatically generated - may contain errors
2 protocols using cat 5005
1
Culturing BEAS-2B Cells for PM Exposure
2
BEAS-2B Cell Culture Methodology
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BEAS-2B cells (ATCC CRL-9609) were cultured from cryopreserved stocks in collagen-coated T-75 culture flasks according to ATCC guidelines. Briefly, cells were seeded at 3,000 cells/cm2 and cultured in 23mL of BEGM Bronchial Epithelial Cell Growth Medium (Lonza, CC-3170), omitting the addition of the gentamicin-amphotericin aliquot to the medium, as recommended by ATCC. Cells were grown at 37°C in a humidified incubator with a 5% CO2 atmosphere, and complete media exchanges were performed every 48 hours. After approximately 4 days, the cultures reached ~70% confluency, and cells were sub-cultured into 6-well or 96-well plates coated with Type 1 collagen (Advanced BioMatrix, Cat#5005), and allowed to attach to the growth surface for 24 hours prior to exposure to particulate matter.
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