Cells grown and treated as indicated were collected, and the total protein was extracted. The following primary antibodies were used: rabbit monoclonal anti-phosphorylated IGF-1R (Tyr1131), rabbit monoclonal anti-IGF-1R, rabbit monoclonal anti-Akt, rabbit monoclonal anti-phosphorylated Akt (Ser473), rabbit monoclonal anti-phosphorylated mTOR (Ser2448), rabbit monoclonal anti-mTOR, rabbit monoclonal anti-p70s6k, or rabbit monoclonal anti-phosphorylated p70s6k (Thr389), rabbit monoclonal anti-S6, or rabbit monoclonal anti-phosphorylated S6 (Ser240/244), rabbit monoclonal anti-AMPK, or rabbit monoclonal anti-phosphorylated AMPK (Thr172) (all from Cell Signaling Technology, Inc.). Horseradish peroxidase-conjugated goat-anti-rabbit antibody (Thermo Scientific) was used as a secondary antibody. The control for equal protein loading was assessed with an anti-β-actin antibody (Cell Signaling Technology, Inc.).
Rabbit monoclonal anti s6
Manufactured by Cell Signaling Technology
Sourced in Germany
Rabbit monoclonal anti-S6 is a primary antibody that recognizes the S6 ribosomal protein. It is suitable for use in Western blotting and immunohistochemistry applications.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit monoclonal anti akt, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Rabbit monoclonal anti phosphorylated akt ser473, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti mtor, by Cell Signaling Technology (1 mentions)
Rabbit polyclonal anti akt, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti sirt1 ab110304, by Abcam (1 mentions)
Mouse monoclonal anti α tubulin t5168, by Merck Group (1 mentions)
Lamin a c, by Cell Signaling Technology (1 mentions)
2 protocols using rabbit monoclonal anti s6
1
Western Blot Analysis of Signaling Pathways
2
Immunoblotting Antibody Validation Protocol
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Immunoblotting was performed as previously reported16 (link),17 (link). The antibodies used were mouse monoclonal anti-VDAC1 (ab14734), mouse monoclonal anti-SDHA (ab14715), mouse monoclonal anti-Rieske (ab14746), mouse monoclonal anti-SIRT1 (ab110304), rabbit polyclonal anti-histone H3 (ab1791) from Abcam (Cambridge, UK); mouse monoclonal anti-HSP60 (SPA-806) from Enzo Life Sciences (Farmingdale, NY); guinea pig polyclonal anti-p62 (GP62-C) from Progen (Heidelberg, Germany); rabbit monoclonal anti-LC3A/B (#12741), rabbit monoclonal anti-phospho-Ser37/46-4EBP1 (#2855), rabbit polyclonal anti-4EBP1 (#9452), rabbit polyclonal anti-phospho-ser235/236 S6 (#2211), rabbit monoclonal anti-S6 (#2217), rabbit polyclonal anti-phospho-Ser473 Akt (#9271), rabbit polyclonal anti-Akt (#9272), rabbit monoclonal anti-FoxO3a (#2497), Lamin A/C (#2032) from Cell Signaling Technologies (Danvers, MA, USA); mouse monoclonal anti-GAPDH (G8795), mouse monoclonal anti-α-tubulin (T5168) from Sigma Aldrich (St. Louis, MO, USA); and rabbit polyclonal anti-acetylated histone H3 (382158) from Millipore (Billerica, MA, USA).
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