IDF-11774 (S8771, purity > 99%; Selleckchem, Houston, TX) was prepared as stock solution in dimethyl sulfoxide (DMSO) at 10 mM and stored at –20°C. In brief, 10 μM IDF-11774 was supplemented in cell culture, while 20 mg/kg/day IDF-11774 was administered orally to mice, according to previous methods (21 (link)).
Idf 11774
Manufactured by Selleck Chemicals
The IDF-11774 is a laboratory instrument designed for conducting various analytical and experimental tasks. It is a versatile piece of equipment that can be utilized across multiple scientific disciplines. The core function of the IDF-11774 is to facilitate controlled and precise measurements, data collection, and sample processing within a laboratory setting.
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2 protocols using idf 11774
1
IDF-11774 Cell and Mouse Assay
2
Protocols for Culturing Mouse Sertoli and Human Kidney Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The TM4 and HEK293T cell line (immortalized mouse Sertoli cells) was purchased from Procell Life Science Technology. TM4 and HEK293T are maintained in Dulbecco’s modified Eagle’s medium (DMEM) (GIBIO, 12100061) supplemented with 10% fetal bovine serum (VISTECH, SE100-011) and 1% penicillin and streptomycin. All cells were cultured in 37 °C humidified incubators with 5% CO2. High glucose culture environment with a glucose concentration of 9.0 g/L. The cells do not have mycoplasma contamination. All treatment times for cells were 24 h if not otherwise specified. Agonists, antagonists, and metabolites used in this study were listed as follows: Glutathione (Selleck, S4606), L-carnitine (Selleck, S2388), NAD+ (Selleck, S2518), Palmitelaidic Acid (MedChemExpress, HY-N2341), Apelin-13 (Abcam, ab141010), Pyr1-Apelin-13 (TargetMol, TP1260), Ulixertinib (Selleck, S7854), AICAR (Selleck, S1802), IDF-11774 (Selleck, S8771).
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