MEG‐01 and its subclone MEG‐01s MB cell lines were obtained from JCRB Cell Bank (Japan), cultured in RPMI1640 medium (Gibco) with 10% FBS and maintained in a humidified atmosphere of 5% CO2 environment at 37°C.
Meg 01
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
The MEG-01 is a cell culture plate that can be used for the cultivation and maintenance of human megakaryocytic cells. It provides a standardized and controlled environment for the growth and study of these specialized blood cells.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Ham s f 12 nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hl 60, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Thp 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Jurkat, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using meg 01
1
Culturing MEG-01 Cell Lines
2
Cell Line Maintenance Protocols for Leukemia Research
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The human leukemia cell lines K562 [28 (link)], HEL [29 (link)], MEG–01 [30 (link)], CMK-11-5 [31 (link)], HL60 [32 (link)], NB4 [33 (link)], Jurkat [34 (link)], Reh [35 (link)], and THP–1 [36 (link)] were obtained from the Japanese Collection of Research Bioresources Cell Bank and maintained in Roswell Park Memorial Institute 1640 medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO2, unless otherwise indicated. The erythropoietin-dependent erythroid cell line UT–7/Epo was kindly provided by Dr. Norio Komatsu and maintained in the same medium supplemented with 10% FBS and 1 U/ml recombinant human erythropoietin (Kyowa Hakko Kirin, Tokyo, Japan) [37 (link)]. The Chinese hamster ovary cell line CHO-TRVb, which lacks functional TFR1, was kindly provided by Dr. Timothy McGraw and maintained in Ham’s F12 nutrient mixture (Life Technologies) supplemented with 5% FBS [38 (link)].
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