Alexa fluor 647

Nab-paclitaxel (Nab, Paclitaxel for Injection (Albumin Bound), provided by CSPC Pharmaceutical, China) was dissolved in 0.9% saline, Tras (Herceptin, provided by Roche Genentech, US) was dissolved in distilled water. Lova (Targetmol, China) was dissolved in DMSO, and Lova sodium (Glpbio, US) was dissolved in ethanol for preparation. RPMI-1640 medium, penicillin (100 U/ml)–streptomycin (100 mg/ml), trypsin-EDTA (0.25%) were purchased from Macgene technologies (China). McCoy 5a medium was purchased from Sigma. Cell counting kit-8, BCA protein assay kit, RIPA lysis buffer, Protease and phosphatase inhibitor cocktail, GAPDH Rabbit Monoclonal Antibody, HER2/ErbB rabbit polyclonal antibody, HRP-labeled Goat Anti-Rabbit IgG(H + L), Annexin V-FITC apoptosis detection kit, cell cycle, and apoptosis analysis kit were purchased from Beyotime technologies (China). Octyl-b-d-glucopyranoside was purchased from Millipore. Anti-Cav-1 antibody was purchased from Abcam. Alexa Fluor 488 and Alexa Fluor 647 secondary antibodies were purchased from Bioss. For transient transfection for Cav-1 knocking down and overexpression, lipofectamine 2000 was purchased from Thermo Fisher, genOFF h-CAV1_1999A was purchased from Ribobio technologies (China), and CAV1_pcDNA3.1(+) were purchased from Genscript technologies (China).