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4 protocols using octanesulfonate sodium

1

Microneedle-Assisted Transdermal Drug Delivery

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NTX-HCl was obtained from Mallinckrodt Pharmaceuticals (Webster Groves, MO, USA). P407 was obtained from Anatrace (Maumee, OH, USA). HEPES and sodium bicarbonate were obtained from Research Products International (Mt. Prospect, IL, USA). Sodium hydroxide (1N), o-phosphoric acid, and methanol Optima® were obtained from Fisher Chemical (Lenexa, KS, USA). Octanesulfonate sodium, Hank’s balanced salts, HPLC grade water, and HPLC grade acetonitrile were obtained from Sigma Aldrich (St. Louis, MO, USA). Snakeskin® cellulose acetate membrane (10,000 MWCO) was obtained from Thermo Scientific (Rockford, IL, USA). Solid stainless steel microneedle arrays of 600 μm or 750 μm length (200 μm width, 75 μm thickness, 1.3 mm inter-needle spacing for both) consisting of 50 projections bent 90° out of plane were purchased from Tech Etch (Plymouth, MA, USA).
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2

Naltrexone Hydrogel Microarray for Transdermal Delivery

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Naltrexone hydrochloride (NTX) was obtained from Mallinckrodt Pharmaceuticals (Webster Groves, MO, USA). P407 was obtained from Anatrace (Maumee, OH, USA). HEPES, methanol, and sodium bicarbonate were obtained from Research Products International (Mt Prospect, IL, USA). Sodium hydroxide (1 N), o-phosphoric acid, polyethylene glycol (PEG, MW 400), and methanol Optima® were obtained from Fisher Chemical (Lenexa, KS, USA). Acetic acid, octanesulfonate sodium, Hank's balanced salts, gentamicin sulfate, dimethyl sulfoxide (DMSO), glycerol, propylene glycol, benzyl alcohol, isopropyl myristate, oleic acid, HPLC grade water, and HPLC grade acetonitrile were obtained from Sigma Aldrich (St Louis, MO, USA). Ethanol was obtained from Decon Laboratories (King of Prussia, PA, USA). Isopropyl palmitate was obtained from Alfa Aesar (Haverhill, MA, USA). Hydroxyethyl cellulose (750 kDa) was obtained from Ashland (Wilmington, DE, USA). Solid stainless steel MN arrays of 600 μm length (56 projections, 200 μm width, 75 μm thickness, 1.3 mm inter-needle spacing) were purchased from Tech Etch (Plymouth, MA, USA). (5-(4,6-Dichlorotriazinyl)aminofluorescein) (5-DTAF) was obtained from Thermo Fisher.
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3

Composite Coatings for Titanium Implants

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The following materials were all purchased from Sigma-Aldrich and used to assemble composite coatings on Ti surface: powders of HAP (particles < 200 nm), chitosan (medium molecular weight (190-310) kDa, degree of deacetylation 75-85 %), and gentamicin sulfate solution (50 mg/mL in dH2O). Pure graphene nanopowder (99.2 %) with average graphene nanoflakes thickness of 12 nm was purchased from Graphene Supermarket, USA. Before EPD, titanium plates (15 × 10 × 0.25 mm, 99.7 %) were mechanically polished and ultrasonicated (15 min in acetone). Methanol, ophtalaldehyde, 2-mercaptoethanol, boric acid, sodium hydroxide, and 2-propanol (all purchased from Merck, Germany) were used for gentamicin derivatization procedure. The mobile phase consisted of sodium octanesulfonate and glacial acetic acid (Merck, Germany).
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4

Biogenic Amine Analysis Protocol

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All chemicals and reagents used were of analytical or HPLC grade or equivalent. Biogenic amine standards such as histamine hydrochloride, putrescine dihydrochloride, tyramine hydrochloride, cadaverine dihydrochloride, and agmatine sulphate were obtained from Sigma (USA). Perchloric acid and boric acid were also obtained from Sigma (France). O-phthalaldehyde (OPA), mercaptoethanol, polyoxyethylene (Brig-35), and sodium octanesulfonate were obtained from Merck (Germany). Double distilled water was used to prepare solutions.
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