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Sc 7706

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Sc-7706 is a laboratory product from Santa Cruz Biotechnology. It is a specific antibody designed for use in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab13970, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Z fix, by Anatech (2 mentions) Ab7817, by Abcam (2 mentions) Goat anti sp c, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 568 donkey anti goat, by Thermo Fisher Scientific (1 mentions) Ab98952, by Abcam (1 mentions) Ab3280, by Abcam (1 mentions) Gtx100443, by GeneTex (1 mentions) Normal goat igg sc 2028, by Santa Cruz Biotechnology (1 mentions) Normal mouse igg sc 2025, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) A 21244, by Thermo Fisher Scientific (1 mentions) Nb100 304, by Novus Biologicals (1 mentions) Ab3786, by Merck Group (1 mentions) A31571, by Thermo Fisher Scientific (1 mentions) Sc 134483, by Santa Cruz Biotechnology (1 mentions) A 11057, by Thermo Fisher Scientific (1 mentions) Ta150032, by OriGene (1 mentions)

5 protocols using sc 7706

1

Lung Tissue Immunofluorescence Staining

Cited 1 time
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Mouse lungs were perfused and inflated with 20 ml/kg aqueous buffered zinc formalin (Z-FIX; Anatech, Battle Creek, MI) immediately following euthanasia and used for paraffin-embedding. Immunofluorescence staining was performed as described previously (Lee et al., 2017 (link)). Paraffin-embedded tissue sections (5 μm) were rehydrated and subjected to antigen retrieval in Tris-HCl buffer (100 mM, pH 9.5). Blocking was performed with 15% BSA in 0.2% Triton-X/PBS at room temperature for 60 min. Sections were incubated with chicken anti-GFP (1:500, Abcam, ab13970) and goat anti-SP-C (1:50, Santa Cruz Biotechnology, sc-7706) overnight at 4°C. The immune complexes were detected using Alexa Fluor 568 donkey anti-goat or Alexa Fluor 488 goat anti-chicken (1:400, Invitrogen) secondary antibodies before sections were counterstained with DAPI. Sections were mounted in Prolong Gold antifade reagent (Invitrogen). Fluorescence images were acquired using a Zeiss microscope.
Zhu B., Wu Y., Huang S., Zhang R., Son Y.M., Li C., Cheon I.S., Gao X., Wang M., Chen Y., Zhou X., Nguyen Q., Phan A.T., Behl S., Taketo M.M., Mack M., Shapiro V.S., Zeng H., Ebihara H., Mullon J.J., Edell E.S., Reisenauer J.S., Demirel N., Kern R.M., Chakraborty R., Cui W., Kaplan M.H., Zhou X., Goldrath A.W, & Sun J. (2021). Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection. Immunity, 54(6), 1200-1218.e9.
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2

Evaluating EMT Markers in RLE-6TN Cells

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The expression levels of proteins involved in EMT (SPC, SMA, E-cad and N-cad) in RLE-6TN cells were analyzed by western blot analysis as mentioned above. The antibody concentrations were as follows: SPC (1:100, goat polyclonal antibody; sc-7706; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), α-SMA (1:500, mouse monoclonal to α-SMA; ab7817; Abcam), E-cad (1:1,000, rabbit polyclonal to E-cad; GTX100443, GeneTex, Irvine, CA, USA), N-cad (1:1,000, mouse monoclonal to N-cad; ab98952; Abcam) or β-actin (1:1,000, mouse monoclonal [ACTN05 (C4)] to actin; ab3280; Abcam). ImageJ software was used to analyze the optical density of the protein bands and then normalized to that of β-actin.
The mRNA expression of SPC, α-SMA, E-cad, N-cad in the RLE-6TN cells was analyzed by real-time PCR as mentioned above. The primers and amplification conditions are shown in Table I. The relative level of mRNA expression was calculated following normalization to β-actin.
Yang H., Fu J., Yao L., Hou A, & Xue X. (2017). Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD. International Journal of Molecular Medicine, 40(5), 1466-1476.
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3

Immunofluorescence Staining of Mouse Lungs

Cited 1 time
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Mouse lungs were perfused and inflated with 20 ml/kg aqueous buffered zinc formalin (Z-FIX; Anatech, Battle Creek, MI) immediately following euthanasia and used for paraffin-embedding. Immunofluorescence staining was performed as described previously (Lee et al., 2017 (link)). Paraffin-embedded tissue sections (5 μm) were rehydrated and subjected to antigen retrieval in Tris-HCl buffer (100 mM, pH 9.5). Blocking was performed with 15% BSA in 0.2% Triton-X/PBS at room temperature for 60 min. Sections were incubated with chicken anti-GFP (1:500, Abcam, ab13970) and goat anti-SP-C (1:50, Santa Cruz Biotechnology, sc-7706) overnight at 4 °C. The immune complexes were detected using Alexa Fluor 568 donkey anti-goat or Alexa Fluor 488 goat anti-chicken (1:400, Invitrogen) secondary antibodies before sections were counterstained with DAPI. Sections were mounted in Prolong Gold antifade reagent (Invitrogen). Fluorescence images were acquired using a Zeiss microscope.
Zhu B., Wu Y., Huang S., Zhang R., Son Y.M., Li C., Cheon I.S., Gao X., Wang M., Chen Y., Zhou X., Nguyen Q., Phan A.T., Behl S., Taketo M.M., Mack M., Shapiro V.S., Zeng H., Ebihara H., Mullon J.J., Edell E.S., Reisenauer J.S., Demirel N., Kern R.M., Chakraborty R., Cui W., Kaplan M.H., Zhou X., Goldrath A.W, & Sun J. (2021). Uncoupling of macrophage inflammation from self-renewal modulates host recovery from respiratory viral infection. Immunity, 54(6), 1200-1218.e9.
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4

Immunofluorescence Staining of RLE-6TN Cells

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The RLE-6TN cells were cultured, respectively on glass slides in the 5 groups, fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton X-100, then washed 3 times with PBS. The sections were subsequently incubated in 5% FBS for 30 min for antigen blocking. The slides were then incubated with a mixture of two primary antibodies [anti-SPC (1:100 diluted, goat poly-clonal antibody; sc-7706; Santa Cruz Biotechnology, Inc.) and α-SMA (1:100 diluted, mouse monoclonal to α-SMA; ab7817; Abcam)] at −4°C overnight. Normal goat IgG (sc-2028) or normal mouse IgG (sc-2025) (both from Santa Cruz Biotechnology, Inc.) was used respectively in place of the primary antibodies in the negative control samples. The slides were then washed 3 times with PBS and then incubated with a mixture of two secondary antibodies [anti-mouse antibody (Alexa Fluor-488, green fluorescence) and anti-goat antibody (Alexa Fluor-594, red fluorescence)] at 37°C for 60 min. Subsequently, the sections were further washed with PBS 3 times and then subjected to DAPI (1:2,000; Sigma-Aldrich, St. Louis, MO, USA) nuclear staining for 5 min. After washing thoroughly with PBS, images of the sections were acquired using a fluorescence microscope at x400 magnification.
Yang H., Fu J., Yao L., Hou A, & Xue X. (2017). Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD. International Journal of Molecular Medicine, 40(5), 1466-1476.
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5

Immunostaining of Lung Tissue Sections

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The following antibodies were used to immunostain FFPE sections: goat Spc (1:100; sc7706; Santa Cruz Biotechnology); rabbit Spc (1:500; ab3786; Millipore); rabbit Pdpn (1:100; sc134483; Santa Cruz Biotechnology); rabbit 53BP1 (1:3500; NB100-304; Novus Biologicals); goat CD34 (1:100; Santa Cruz Biotechnology); rabbit eGFP (1:1000; TA150032; Origene); mouse αSMA (1:3000; A2547; Sigma Aldrich); rabbit ΔNp63 (1:100; 619001; Biolegend); donkey anti-goat Alexa 568 (1:2000; A11057; Thermo Fisher Scientific); goat anti-rabbit Alexa 647 (1:2000, A21244; Thermo Fisher Scientific); donkey anti-mouse (1:2000; A31571; Thermo Fisher Scientific). All sections were counterstained with DAPI. Whole slide immunofluorescence imaging was performed in the Digital Histology Shared Resource at Vanderbilt University Medical Center using a Leica Apiro Versa whole slide scanner that provides high resolution wide field images of ranging from 0.05x to 40x. Entire lung sections were first inspected at a low magnification. Following the initial inspection each entire lung section was then inspected at 20 to 40x. Images were captured as jpeg files. Confocal images of immunofluorescence staining were acquired using an Olympus FV-1000 inverted confocal microscope using a 60x/1.45 Plan-Apochromat oil immersion objective lens. Images were captured as jpeg files.
Traver G., Mont S., Gius D., Lawson W.E., Ding G.X., Sekhar K.R, & Freeman M.L. (2017). Loss of Nrf2 promotes alveolar type 2 cell loss in irradiated, fibrotic lung. Free radical biology & medicine, 112, 578-586.
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