B6.129 icr tg cag ecfp ck6nagy j

KRT14-Kitl*4XTG2Bjl (Krt14-Kitl*) mice were a gift from BJ Longley, University of Wisconsin. PMEL TCR transgenic mice are Thy1.1⁺, and were obtained from The Jackson Laboratory, stock no. 005023, B6.Cg Thy1^a/CyTg(TcraTcrb)8Rest/J. GFP-PMEL and CFP-PMEL TCR transgenic mice were produced by crossing PMEL transgenic mice with DPE^GFP mice, which express GFP in T cells (provided by U. von Andrian, Harvard Medical School, Boston, MA), or with CAG-ECFP [The Jackson Laboratory, stock no. 004218, B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J], respectively. CFP-PMEL TCR transgenic mice were backcrossed to B6 for a total of 10 generations. Cxcr3−/− PMEL TCR transgenic mice were produced by crossing GFP-PMEL mice with the B6.129P2-Cxcr3^tm1Dgen/J strain (The Jackson Laboratory, stock no. 005796). To develop Cxcl9- and Cxcl10-deficient recipients, B6.129S4-Cxcl9^tm1Jmf (provided by Joshua M. Farber, NIAID, Bethesda, MD) and B6.129S4-Cxcl10^tm1Adl/J (The Jackson Laboratory, stock no. 006087) mice were crossed with Krt14-Kitl*. For consistency, the Krt14-Kitl* allele was heterozygous on all mice used in experiments. All mice were on a C57BL/6J background, were maintained in pathogen-free facilities at UMMS, and procedures were approved by the UMMS Institutional Animal Care and Use Committee and in accordance with the NIH Guide for the Care and Use of Laboratory Animals.

Mice aged between 3 to 4 months were counted as young and mice aged over 20 months were considered as old. Young and Old C57Bl/6-β-actin-EGFP mice were mostly used to obtain MuSCs in this study were initially provided by Dr. Amy Wagers (Harvard University, Cambridge, MA) and maintained in the Jang lab mouse colony. B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J and B6.Cg-Gt(ROSA)26Sor^tm1(EYFP)Cos mice were purchased from the Jackson Laboratory. Sod1^−/− mice were initially provided by Dr. Holly Van Remmen (Oklahoma Medical Research Foundation). Female mice were used in this study. All mice were housed, aged, and/or bred in the specific pathogen-free condition in Physiological Research Laboratory (PRL) at Georgia Institute of Technology. All procedures were performed in accordance with the approval of the Institutional Animal Care and Use Committee (IACUC) at Georgia Institute of Technology.