Amino acid analysis was performed on dried blood spots using an ABI 3200 Q-TRAP LC-MS/MS system (Applied Biosystems) as previously described.69 (link) Briefly, 25 μL of blood was applied to an S&S Grade 903 filter, dried for 3 h, and then sealed in a plastic bag at −20°C until testing. A 3-mm diameter disc was obtained from each dried blood card and placed in a 96-well polyprolene plate. Methanol (100 μL) containing isotopic internal standards was added to each well followed by incubation at 45°C for 45 min with gentle agitation. After 20 min at room temperature, 75 μL aliquots were transferred to a new plate and covered with an aluminum membrane before use.
Abi 3200 q trap lc ms ms system
Manufactured by Thermo Fisher Scientific
The ABI 3200 Q-TRAP LC-MS/MS system is a liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument designed for qualitative and quantitative analysis of a wide range of analytes. It combines a triple quadrupole mass analyzer with a linear ion trap, providing enhanced sensitivity, selectivity, and flexibility for numerous applications.
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Lab products found in correlation
2 protocols using abi 3200 q trap lc ms ms system
1
Dried Blood Spot Amino Acid Analysis
2
Biochemical Analysis of Blood Serum
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Blood was obtained via puncture of the ear vein using heparinized tubes. Serum was separated by centrifugation at 900 × g for 20 min and stored at −80 °C prior to biochemistry and amino acid analysis. Concentrations of ALT, AST, and triglycerides were measured with a CL-8000 Hitachi 7180 automatic biochemical analyzer (Shimadzu, Kyoto, Japan). Tyrosine was quantified by liquid chromatography-mass spectrometry using the ABI 3200 Q TRAP LC-MS/MS system (Applied Biosystems, Foster City, CA).
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