Formalin-fixed, paraffin-embedded specimens were collected, and a routine H&E slide was first evaluated. Immunohistochemical staining was done on 5 μm-thick paraffin-embedded sections using mouse anti-Merlin (Abcam), rabbit anti-GPX4 (Abcam), rabbit anti-PTGS2 (Cell Signaling), mouse anti-Ki67 (Cell Signaling), rabbit anti-ACSL4 (Thermo Fisher), rabbit anti-TFRC (Abcam), and rabbit anti-YAP (Cell Signaling) antibodies with a standard avidin-biotin HRP detection system according to the instructions of the manufacturer (anti-mouse/rabbit HRP-DAB Cell & Tissue Staining Kit, R&D Systems Minneapolis, MN). Tissues were counterstained with haematoxylin, dehydrated, and mounted. In all cases, antigen retrieval was done with the BD Retrievagen Antigen Retrieval Systems as per instructions of the manufacturer.
Rabbit anti ptgs2
Manufactured by Cell Signaling Technology
Rabbit anti-PTGS2 is a primary antibody that recognizes the PTGS2 (prostaglandin-endoperoxide synthase 2) protein. PTGS2 is an enzyme that catalyzes the formation of prostanoids, which are important mediators of inflammation and other physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti merlin, by Abcam (2 mentions)
Rabbit anti gpx4, by Abcam (2 mentions)
Mouse anti ki67, by Cell Signaling Technology (2 mentions)
Rabbit anti acsl4, by Thermo Fisher Scientific (2 mentions)
Rabbit anti tfrc, by Abcam (2 mentions)
Anti mouse rabbit hrp dab cell tissue staining kit, by R&D Systems (2 mentions)
Rabbit anti yap, by Cell Signaling Technology (2 mentions)
2 protocols using rabbit anti ptgs2
1
Immunohistochemical Analysis of Formalin-Fixed Tissues
2
Immunohistochemical Analysis of Formalin-Fixed Tissues
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