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Cfhenwps

Manufactured by Proteintech
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The CFHENWPS is a laboratory equipment product. It serves a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Sample buffer, by Bio-Rad (2 mentions) Dtssp, by Thermo Fisher Scientific (2 mentions) Cplaygtw, by Proteintech (2 mentions) Recombinant cd59, by R&D Systems (2 mentions) Gelcode blue stain reagent, by Thermo Fisher Scientific (2 mentions) Native sample buffer, by Bio-Rad (2 mentions) Nativepage running buffer 20, by Thermo Fisher Scientific (2 mentions)

4 protocols using cfhenwps

1

CD59 Cross-Linking Assay Protocol

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Peptide F (2 μg) (CFHENWPS, Proteintech) and control peptide (2 μg) (CPLAYGTW, Proteintech) were incubated separately with 1 μg of recombinant CD59 (R&D Systems) in 21 μl of PBS on a slow shaker overnight at 4°C. The cross-linking reagent 3,3′-dithiobis[sufosuccinimidylpropionate] (DTSSP) (Thermo Scientific) was added to a final concentration of 2 mM. After rotation at room temperature for 1 hour, the samples were diluted in 2× sample buffer (Bio-Rad) with β-mercapto-ethanol and loaded on 15% SDS-PAGE.
Emin M., Wang G., Castagna F., Rodriguez-Lopez J., Wahab R., Wang J., Adams T., Wei Y, & Jelic S. (2016). Increased internalization of complement inhibitor CD59 may contribute to endothelial inflammation in obstructive sleep apnea. Science translational medicine, 8(320), 320ra1.
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2

Peptide-Protein Interaction Analysis

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HUVEC whole-cell lysate (100 μg was incubated with 30 μg of peptide F (CFHENWPS, Proteintech) at 4°C for 2 hours. The mixture was diluted with 2× native sample buffer (Bio-Rad) and loaded on a 10% native gel in running buffer without SDS [NativePAGE Running Buffer (20×), Thermo Scientific]. The gel was transferred to a nitrocellulose membrane and probed with antibodies directed against peptide F (rabbit polyclonal antibody, 1:500; Proteintech). A duplicate gel was stained with GelCode Blue Stain Reagent (Thermo Scientific). The corresponding band of interest was cut and sent to Columbia University Protein Chemistry Core Facility for mass spectrometry.
Emin M., Wang G., Castagna F., Rodriguez-Lopez J., Wahab R., Wang J., Adams T., Wei Y, & Jelic S. (2016). Increased internalization of complement inhibitor CD59 may contribute to endothelial inflammation in obstructive sleep apnea. Science translational medicine, 8(320), 320ra1.
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3

CD59 Cross-Linking Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peptide F (2 μg) (CFHENWPS, Proteintech) and control peptide (2 μg) (CPLAYGTW, Proteintech) were incubated separately with 1 μg of recombinant CD59 (R&D Systems) in 21 μl of PBS on a slow shaker overnight at 4°C. The cross-linking reagent 3,3′-dithiobis[sufosuccinimidylpropionate] (DTSSP) (Thermo Scientific) was added to a final concentration of 2 mM. After rotation at room temperature for 1 hour, the samples were diluted in 2× sample buffer (Bio-Rad) with β-mercapto-ethanol and loaded on 15% SDS-PAGE.
Emin M., Wang G., Castagna F., Rodriguez-Lopez J., Wahab R., Wang J., Adams T., Wei Y, & Jelic S. (2016). Increased internalization of complement inhibitor CD59 may contribute to endothelial inflammation in obstructive sleep apnea. Science translational medicine, 8(320), 320ra1.
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4

Peptide-Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVEC whole-cell lysate (100 μg was incubated with 30 μg of peptide F (CFHENWPS, Proteintech) at 4°C for 2 hours. The mixture was diluted with 2× native sample buffer (Bio-Rad) and loaded on a 10% native gel in running buffer without SDS [NativePAGE Running Buffer (20×), Thermo Scientific]. The gel was transferred to a nitrocellulose membrane and probed with antibodies directed against peptide F (rabbit polyclonal antibody, 1:500; Proteintech). A duplicate gel was stained with GelCode Blue Stain Reagent (Thermo Scientific). The corresponding band of interest was cut and sent to Columbia University Protein Chemistry Core Facility for mass spectrometry.
Emin M., Wang G., Castagna F., Rodriguez-Lopez J., Wahab R., Wang J., Adams T., Wei Y, & Jelic S. (2016). Increased internalization of complement inhibitor CD59 may contribute to endothelial inflammation in obstructive sleep apnea. Science translational medicine, 8(320), 320ra1.
+ Open protocol
+ Expand

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