Hp 1100 series hplc

The quantitative analyses of capsaicinoids present in the extracts of the three hot pepper samples (RP1, RP2 and RP3) were carried out through HPLC–UV, using an HP 1100 Series HPLC (Agilent, Palo Alto, CA, USA) equipped with a binary pump (G-1312A) and a UV detector (G-1314A). The chromatographic conditions were as described for the HPLC–MS system, except that phenols were separated on a XBridge BEH C18 column (130 Å, 5 mm, 4.6 mm × 150 mm) (Waters, Milford, MA, USA) at a flow rate of 1 mL min⁻¹. The detection wavelength was 280 nm. The quantification of capsaicinoids was performed using external calibration curves generated via the repeated injections of a fixed volume of capsaicin standard solutions in a concentration range of 0.005–0.1 µg/µL with four different concentration levels and duplicate injections at each level. All samples were analysed in duplicate. The results were expressed as g kg⁻¹ DW.

The quantitative analyses of capsaicinoids present in the extracts of the three hot pepper samples (RP1, RP2 and RP3) were carried out through HPLC–UV, using an HP 1100 Series HPLC (Agilent, Palo Alto, CA, USA) equipped with a binary pump (G-1312A) and a UV detector (G-1314A). The chromatographic conditions were as described for the HPLC–MS system, except that phenols were separated on a XBridge BEH C18 column (130 Å, 5 mm, 4.6 mm × 150 mm) (Waters, Milford, MA, USA) at a flow rate of 1 mL min⁻¹. The detection wavelength was 280 nm. The quantification of capsaicinoids was performed using external calibration curves generated via the repeated injections of a fixed volume of capsaicin standard solutions in a concentration range of 0.005–0.1 µg/µL with four different concentration levels and duplicate injections at each level. All samples were analysed in duplicate. The results were expressed as g kg⁻¹ DW.