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A1rs confocal microscope

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The A1RS confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a fast, resonant scanner that enables rapid image acquisition, and a range of sophisticated optics and detectors for high-resolution, multi-channel imaging. The A1RS is capable of capturing detailed, high-quality images of various samples, making it a versatile tool for a wide range of scientific and research applications.

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Lab products found in correlation

Eclipse ti u, by Nikon (4 mentions) Guinea pig anti insulin, by Agilent Technologies (4 mentions) Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Texas red x phalloidin, by Thermo Fisher Scientific (3 mentions) Blue fluorescent hoechst, by Thermo Fisher Scientific (3 mentions) Live cell imaging solution, by Thermo Fisher Scientific (3 mentions) Vectashield mounting medium with dapi, by Vector Laboratories (2 mentions) 4 6 diamidine 2 phenylindole dihydrochloride dapi, by Merck Group (2 mentions) Shandon immu mount, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Abcam (1 mentions) Alexa fluor 594 goat anti rat igg, by Thermo Fisher Scientific (1 mentions) Rat anti mouse cd31 antibody, by BD (1 mentions) Duolink in situ red starter kit mouse rabbit, by Merck Group (1 mentions) Mouse anti rip1, by BD (1 mentions) Rabbit anti rip3, by ProSci (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Anti active caspase 3 pab, by Promega (1 mentions) 4t1 cell, by American Type Culture Collection (1 mentions) Rabbit anti cleaved caspase 3 primary antibody, by Cell Signaling Technology (1 mentions) Ab3421, by Abcam (1 mentions)

Close spelling variants of this product

A1R confocal microscope (1179 citations) Confocal microscope (523 citations) A1R microscope (119 citations) A1R confocal (60 citations) A1RS confocal microscope system (5 citations) A1RS high-definition (HD) confocal microscope (2 citations) A1rs HD Confocal Microscope (2 citations)

37 protocols using a1rs confocal microscope

1

Larval Wing Disc Confocal Imaging

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Embryos were dechorionated and analyzed under halocarbon oil to determine stage. Third instar larval (L3) wing discs were dissected and mounted in PBS. Confocal images were acquired on an A1R-S Confocal Microscope (Nikon) with 20x objective. Images were analyzed using NIS-Elements AR software. Z-stacks were flattened using the Maximum Intensity Z-projection function.
Hamm D.C., Larson E.D., Nevil M., Marshall K.E., Bondra E.R, & Harrison M.M. (2017). A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster. PLoS Genetics, 13(12), e1007120.
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2

Visualizing 3T3 Cell Cytoskeleton and Nucleus

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After 3T3 cells were cultured on encapsulation or cell plates in 24-well plates, cell morphology was observed directly using an inverted optical microscope (Nikon Eclipse Ti–U, Japan). The cytoskeleton and nucleus were stained with Texas red-X phalloidin (591/608 nm) and blue fluorescent Hoechst (352/461 nm) (ThermoFisher Scientific), respectively. The samples were fixed with 2–4% formaldehyde for 15 min and then rinsed three times with prewarmed PBS. The samples were incubated with Texas red-X phalloidin (100 nM) and Hoechst (50 nM) for 30 min at 37 °C. After staining, cells were rinsed with prewarmed buffer for 3 times and imaged using a Nikon A1RS confocal microscope (Figure S5a).
Yao G., Jiang D., Li J., Kang L., Chen S., Long Y., Wang Y., Huang P., Lin Y., Cai W, & Wang X. (2019). Self-Activated Electrical Stimulation for Effective Hair Regeneration via a Wearable Omnidirectional Pulse Generator. ACS nano, 13(11), 12345-12356.
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3

Pancreas Islet Cell Proliferation

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For each mouse (n = 4-5 per genotype) four to five 10 μm thick whole pancreas sections, each 150 μm apart, were stained with Guinea pig anti-insulin (Agilent), Rabbit anti-ki67 (CST), and DAPI. 18-26 islets with at least 3 coming from each section were imaged using a Nikon A1RS confocal microscope at 20x magnification. Ki-67 +/Insulin+ cells in all islets from each mouse were counted and divided by the sum of insulin area across the same islets to determine the relative beta cell proliferation in each mouse.
Sdao S.M., Ho T., Poudel C., Foster H.R., De Leon E.R., Adams M.T., Lee J.H., Blum B., Rane S.G, & Merrins M.J. (2021). CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent KATP channel closure. Cell reports, 34(4), 108690.
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4

Quantifying Pancreatic Islet Composition

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For each mouse (n = 4-5 per genotype), eight 10 μm thick whole pancreas sections, each 150 μm apart, were stained with Guinea pig anti-insulin (Agilent), Rabbit anti-glucagon (CST), and DAPI. Whole sections were then imaged on a Nikon A1RS confocal microscope at 10x magnification. The area of insulin, glucagon, and DAPI staining was measured in μm2 using FIJI’s auto thresholding and Anal-zeParticles functions. β-Cell and α-cell mass were determined for each animal by dividing the sum of insulin or glucagon area across all sections imaged by the sum of the area of DAPI across those same sections.
Sdao S.M., Ho T., Poudel C., Foster H.R., De Leon E.R., Adams M.T., Lee J.H., Blum B., Rane S.G, & Merrins M.J. (2021). CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent KATP channel closure. Cell reports, 34(4), 108690.
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5

Immunofluorescent Detection of DNA Damage

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Cells were permeabilized with 0.1% Triton-X 100, blocked with goat serum, and incubated with anti-phospho-H2AX primary antibody. Cells were probed with Alexa Fluor 555 conjugated secondary antibody (CST #4413). A Nikon A1RS confocal microscope was used.
Fisher M.M., SenthilKumar G., Hu R., Goldstein S., Ong I., Miller M., Brennan S.R., Kaushik S., Abel L., Nickel K.P., Iyer G., Harari P.M., Kimple R.J, & Baschnagel A.M. (2020). Fibroblast Growth Factor Receptors as Targets for Radiosensitization in Head and Neck Squamous Cell Carcinomas. International journal of radiation oncology, biology, physics, 107(4), 793-803.
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6

Pancreas Islet Cell Proliferation

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For each mouse (n = 4-5 per genotype) four to five 10 μm thick whole pancreas sections, each 150 μm apart, were stained with Guinea pig anti-insulin (Agilent), Rabbit anti-ki67 (CST), and DAPI. 18-26 islets with at least 3 coming from each section were imaged using a Nikon A1RS confocal microscope at 20x magnification. Ki-67 +/Insulin+ cells in all islets from each mouse were counted and divided by the sum of insulin area across the same islets to determine the relative beta cell proliferation in each mouse.
Sdao S.M., Ho T., Poudel C., Foster H.R., De Leon E.R., Adams M.T., Lee J.H., Blum B., Rane S.G, & Merrins M.J. (2021). CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent KATP channel closure. Cell reports, 34(4), 108690.
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7

Immunostaining of Mouse Anterior Segment

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Mouse tissues were acquired following protocols that were ethically reviewed and approved by the University of Wisconsin–Madison School of Medicine and Public Health Institutional Animal Care and Use Committee, and adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Anterior segments from 1-week-old wild-type (WT) mouse eyes were flat-mount immunostained for SVEP1 and CD31 as detailed in the Supplementary Materials. Briefly, enucleated eyes were immersion fixed in 2% PFA, anterior eye cups dissected, and tissues immunostained using rat anti-mouse CD31 antibody (Cat # 553370, BD Pharmingen, San Jose, CA), rabbit anti-human SVEP1 antibody (Cat # PA5-54436, Thermo Fisher Scientific, Waltham, MA), goat anti-rat IgG Alexa Fluor 594 (Cat # A-11007, Thermo Fisher Scientific), and goat anti-rabbit IgG Alexa Fluor 488 (Cat # ab150081, Abcam, Cambridge, UK) secondary antibodies. Control tissues were also processed without the addition of primary antibodies. Eye cups were washed, flattened on glass coverslips, and mounted with Shandon Immu-Mount (Thermo Fisher Scientific). SC imaging was performed using a Nikon A1RS confocal microscope.
The specificity of the anti-SVEP1 antibody was also verified via immunohistochemical staining sections of human placenta (Supplementary Fig. S3).
Young T.L., Whisenhunt K.N., Jin J., LaMartina S.M., Martin S.M., Souma T., Limviphuvadh V., Suri F., Souzeau E., Zhang X., Dan Y., Anagnos E., Carmona S., Jody N.M., Stangel N., Higuchi E.C., Huang S.J., Siggs O.M., Simões M.J., Lawson B.M., Martin J.S., Elahi E., Narooie-Nejad M., Motlagh B.F., Quaggin S.E., Potter H.D., Silva E.D., Craig J.E., Egas C., Maroofian R., Maurer-Stroh S., Bradfield Y.S, & Tompson S.W. (2020). SVEP1 as a Genetic Modifier of TEK-Related Primary Congenital Glaucoma. Investigative Ophthalmology & Visual Science, 61(12), 6.
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8

Quantifying Autophagy via GFP-mKate2-LC3 Assay

Cited 2 times
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We followed the method as we recently reported [24 (link)] with minor modifications. We used an updated version of GFP-RFP-LC3 vector, namely, pHluorin (GFP variant)-mKate2 in tandem with human LC3 [28 (link)] (Addgene, #61460, product name FUGW-PK-hLC3). Briefly, cells were seeded onto glass-bottom dishes (NuncTM, 150680), stabilized for 24 h, and then transfected with the vector for 24 h. The cells were then treated with PBS or 5 mM NaIO3 for 24 h and stained with 10 μM Hoechst 33342 (ThermoFisher, H3570) prior to live cell confocal microscopy. Images were acquired with a Nikon A1RS confocal microscope under a 40× oil objective lens.
Shen H., Li J., Heisler-Taylor T., Makin R., Yang H., Mavlyutov T.A., Gelfand B., Cebulla C.M, & Guo L.W. (2021). TMEM97 ablation aggravates oxidant-induced retinal degeneration. Cellular signalling, 86, 110078.
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9

Proximity Ligation Assay for RIP1-RIP3 Interaction

Cited 1 time
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Proximity ligation assay was performed following our published method [29 (link)], using the Duolink® In Situ Red Starter Kit (Mouse/ Rabbit), which was purchased from Sigma-Aldrich (DUO92101). Cryosections were blocked with 5% normal donkey serum in PBS containing 0.1 Triton-X 100 for 1 h at room temperature. Mouse anti-RIP1 (BD Transduction Laboratories, 610,459, 1:100) and Rabbit anti-RIP3 (ProSci, 2283, 1:100) were then incubated with the sections overnight. PLA® probes incubation, ligation, amplification, and mounting followed manufacturer’s instructions. Images were taken and analyzed using a Nikon A1RS confocal microscope.
Yang H., Fu Y., Liu X., Shahi P.K., Mavlyutov T.A., Li J., Yao A., Guo S.Z., Pattnaik B.R, & Guo L.W. (2017). Role of the sigma-1 receptor chaperone in rod and cone photoreceptor degenerations in a mouse model of retinitis pigmentosa. Molecular Neurodegeneration, 12, 68.
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10

Quantifying Pancreatic Islet Composition

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For each mouse (n = 4-5 per genotype), eight 10 μm thick whole pancreas sections, each 150 μm apart, were stained with Guinea pig anti-insulin (Agilent), Rabbit anti-glucagon (CST), and DAPI. Whole sections were then imaged on a Nikon A1RS confocal microscope at 10x magnification. The area of insulin, glucagon, and DAPI staining was measured in μm2 using FIJI’s auto thresholding and Anal-zeParticles functions. β-Cell and α-cell mass were determined for each animal by dividing the sum of insulin or glucagon area across all sections imaged by the sum of the area of DAPI across those same sections.
Sdao S.M., Ho T., Poudel C., Foster H.R., De Leon E.R., Adams M.T., Lee J.H., Blum B., Rane S.G, & Merrins M.J. (2021). CDK2 limits the highly energetic secretory program of mature β cells by restricting PEP cycle-dependent KATP channel closure. Cell reports, 34(4), 108690.
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