1 2 dipalmitoyl sn glycero 3 phospho rac 1 glycerol sodium salt dppg

Egg Phosphatidylcholine (PC), CAS: 8002–43–5, 1,2-Dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DPPG), CAS: 67232–81–9, 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), CAS: 63–89–8, and Cholesterol (Chol), CAS: 57–88–5, were obtained from Sigma-Aldrich Company Ltd. (Poole, UK). 1,2-dioleoyl-sn-glycero-3-phsphoEthanolamine (DOPE), CAS: 4004–05–1, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP), CAS: 144189–73–1, dimethyldioctadecylammonium bromide (DDA), CAS: 3700–67–2, and trehalose 6,6-dibehenate (TDB), CAS: 66758–35–8 were purchased from Avanti Polar Lipids, Inc., (Alabaster, AL), purity >99% (Table I). Ethanol, CAS: 64–17–5, and mEthanol, CAS: 67–56–1, were obtained from Fisher Scientific (Loughborough, UK). TRIS Ultra Pure, CAS: 77–86–1, was obtained from ICN Biomedicals, Inc., (Aurora, Ohio, US). Propofol (2,6-Bis(isopropyl)phenol), CAS: 2078–54–8, and ovalbumin (chicken egg), CAS: 9006–59–1, were obtained from Sigma-Aldrich Company Ltd., (Poole, UK). Ultrafiltration regenerated cellulose membranes (p\n: U2755–10AE) were obtained from Sigma-Aldrich Company Ltd., (Poole, UK) (10 kDa, pore size 0.22 µm), and Biomax polyethersulfone ultrafiltration membrane discs with 300 kDa cutoff, pore size 0.45 µm (p\n: PBMK06210) from Merck Milipore (Darmstadt, Germany).

The synthetic phospholipid 1,2-dipalmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt (DPPG) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The polyfluorenes HTMA-PFP (M_n (g·mol⁻¹) = 4170; M_w (g·mol⁻¹) = 8340), HTMA-PFBT (M_n (g·mol⁻¹) = 4584; M_w (g·mol⁻¹) = 8531) and HTMA-PFNT (M_n (g·mol⁻¹) = 4507; M_w (g·mol⁻¹) = 8990) were synthesized and subsequently characterized in our laboratory [43 (link),44 (link),47 (link)]. Stock solutions of the polyfluorenes were dissolved in dimethyl sulfoxide (DMSO) with a final concentration of 3.65 × 10⁻⁴ M for HTMA-PFP and HTMA-PFNT, and 6.24 × 10⁻⁴ M for HTMA-PFBT (in repeat units), and stored at −20 °C before use. The dye 5(6)-carboxyfluorescein (CF) was purchased from Sigma-Aldrich (St. Louis, MO, USA), as well as the fluorescent membrane probe 1,6-diphenyl-1,3,5-hexatriene (DPH), and the quencher 9,10-anthraquinone-2,6-disulfonic acid (AQS). Stock solutions of the three compounds were prepared in dimethyl sulfoxide (DMSO) (1.25 M), dimethylformamide (DFM) (1 mM) and water (5 mM), respectively. Phosphate buffer solution (50 mM, 0.1 M NaCl, pH 7.4) was prepared in Milli-Q water. The rest of the chemicals were of spectroscopic or analytical reagent grade.