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Shrnas in the pgipz vector for tsc1

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ShRNAs in the pGIPZ vector for TSC1 are a set of small hairpin RNA (shRNA) constructs designed to target the TSC1 gene. The pGIPZ vector is a lentiviral expression system that enables stable knockdown of target genes. These ShRNAs can be used for gene expression studies and functional analysis of the TSC1 gene.

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Lab products found in correlation

Shrnas in the plko 1 puro vector for tfam, by Merck Group (2 mentions) Trcn0000016095, by Merck Group (2 mentions) Trcn0000016093, by Merck Group (2 mentions)

Close spelling variants of this product

PGIPZ (23 citations) PGIPZ vector (11 citations) ShRNA vectors (2 citations)

2 protocols using shrnas in the pgipz vector for tsc1

1

Lentiviral RNAi Knockdown Protocol

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Lentiviral RNAi was performed as described2 (link). The shRNAs in the pLKO.1-puro vector for TFAM (sh1: TRCN0000016095; sh2: TRCN0000016093) and PHB2 (sh1: TRCN0000060920; sh2: TRCN0000060921) were obtained from Sigma-Aldrich. The shRNAs in the pGIPZ vector for TSC1 (sh1: V2LHS_92624) and TSC2 (sh1: V2LHS_233694; sh2: V2LHS_93624) were obtained from GE Dharmacon. Sequences of shRNAs are in Supplementary Table 7.
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
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2

Lentiviral RNAi Knockdown Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral RNAi was performed as described2 (link). The shRNAs in the pLKO.1-puro vector for TFAM (sh1: TRCN0000016095; sh2: TRCN0000016093) and PHB2 (sh1: TRCN0000060920; sh2: TRCN0000060921) were obtained from Sigma-Aldrich. The shRNAs in the pGIPZ vector for TSC1 (sh1: V2LHS_92624) and TSC2 (sh1: V2LHS_233694; sh2: V2LHS_93624) were obtained from GE Dharmacon. Sequences of shRNAs are in Supplementary Table 7.
Liu X., Zhang Y., Ni M., Cao H., Signer R.A., Li D., Li M., Gu Z., Hu Z., Dickerson K.E., Weinberg S.E., Chandel N.S., DeBerardinis R.J., Zhou F., Shao Z, & Xu J. (2017). Regulation of Mitochondrial Biogenesis in Erythropoiesis by mTORC1-Mediated Protein Translation. Nature cell biology, 19(6), 626-638.
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