Human umbilical vein endothelial cells (HUVEC) have been isolated from human umbilical cords [34 ] and seeded in 96-well plate at the density of 5*103 cells/well. The cells were incubated with HMGA1, FOXM1 and HMGA1/FOXM1 conditioned medium and the control medium for 18 h. Normal Human Serum 10% was used as conditioned medium for the positive control experiments. After a washing step, the cells were fixed and permeabilized with Fix&Perm kit (Nordic-MUbio) for 15 min at room temperature in the dark. After two washing steps, the cells were incubated with the α-Ki-67 antibody (Dako), followed by the incubation with the secondary antibody α-mouse FITC (Dako). The cells were washed twice and lysed. Measurements were performed using Infinite200 (Tecan).
α ki 67 antibody
Manufactured by Agilent Technologies
The α-Ki-67 antibody is a laboratory reagent used to detect the Ki-67 protein, a cellular marker associated with cell proliferation. It is commonly used in immunohistochemistry and other immunoassays to identify and quantify actively dividing cells.
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2 protocols using α ki 67 antibody
1
Analyzing HUVEC Proliferation by Ki-67
2
Melanoma Cell Invasion into Dermal Reconstructs
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