Antivinculinantibody v9131

Manufactured by Merck Group

Sourced in Germany

Antivinculinantibody (V9131) is a laboratory product produced by Merck Group. It is a primary antibody that recognizes the protein vinculin, which is an important component of cell-cell and cell-matrix junctions. This antibody can be used for the detection and localization of vinculin in various cell and tissue samples through techniques such as Western blotting, immunohistochemistry, and immunocytochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Sds page gel, by Thermo Fisher Scientific (1 mentions) Dimethyl α ketoglutarate, by Merck Group (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Anti sox9 antibody sc 166505, by Santa Cruz Biotechnology (1 mentions) Anti ssea4, by Santa Cruz Biotechnology (1 mentions) Anti actin antibody sc 1615, by Santa Cruz Biotechnology (1 mentions) Anti phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions) Anti nestin mab353, by Merck Group (1 mentions) Anti flag antibody f3165, by Merck Group (1 mentions) γ h2ax 2577, by Cell Signaling Technology (1 mentions)

3 protocols using antivinculinantibody v9131

Comprehensive Reagent Acquisition for Cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

968 was obtained from Chembridge (San Diego, CA), while BPTES was
a kind gift from Dr. Scott Ulrich (Ithaca College, Ithaca, NY). MDC
and dimethyl-α-ketoglutarate were purchased from Sigma (St.
Louis, MO). T101 and Z-Don were purchased from Zedira GmbH (Darmstadt,
Germany). All of the cell lines used in this study were obtained from
the ATCC (Manassas, VA). Cell culture reagents and SDS-PAGE gels were
obtained from Invitrogen (Carlsbad, CA). Western Lighting Plus ECL
was obtained from PerkinElmer (Waltham, MA). The anti-TG2 cocktail
antibody (MS-300-P) was from Neomarkers (Fremont, CA), and the antivinculin
antibody (V9131) was from Sigma. The HRP-conjugated antirabbit IgG
(70745) was from Cell Signaling (Danvers, MA), and the HRP-conjugated
antimouse IgG (NA931-1 ML) was from GE Healthcare Life Sciences (Pittsburgh,
PA). All of the other reagents were obtained from Thermo Fisher Scientific
(Waltham, MA).

Katt W.P., Antonyak M.A, & Cerione R.A. (2014). Simultaneously Targeting Tissue Transglutaminase and Kidney Type Glutaminase Sensitizes Cancer Cells to Acid Toxicity and Offers New Opportunities for Therapeutic Intervention. Molecular Pharmaceutics, 12(1), 46-55.

+ Open protocol

+ Expand

Antibody Profiling for Cartilage Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti–type I collagen cleavage site antibody (#0217-050) was purchased from ImmunoGlobe. Anti-Flag antibody (F3165) and anti-vinculin antibody (V9131) were purchased from Sigma-Aldrich. Normal mouse IgG (sc-2025), normal rabbit IgG (sc-2027), anti–phospho-JNK Thr¹⁸³/Tyr¹⁸⁵ antibody (sc-6254), anti–phospho-p38 Tyr¹⁸² antibody (sc-166182), anti-COL3A1 antibody (sc-271249), anti-actin antibody (sc-1615), anti-SOX9 antibody (sc-166505), and anti-SSEA4 (sc-21704) were purchased from Santa Cruz Biotechnology. Anti-fibronectin antibody (A0245) was purchased from DAKO. Anti-CTRP3 antibody (ab36870), anti-MMP13 (ab51072), anti-CD146 (ab75769), and anti–chondroitin sulfate (ab11570) were purchased from Abcam. Anti–phospho-Akt Ser⁴⁷³ (#9271), anti-Akt (pan) (#4685), and anti–phospho-Erk1/2 Thr²⁰²/Tyr²⁰⁴ (#4377) were purchased from Cell Signaling Technology. Anti-ACAN antibody (AB1031), anti–type II collagen antibody (MAB8887), and anti-nestin (MAB353) were purchased from Millipore. Rabbit anti-mouse IgG + IgM (H&L) conjugated with DyLight 488 (711-065-151) and goat anti-rabbit IgG + IgM (H&L) conjugated with DyLight 594 (111-585-003) were purchased from Jackson ImmunoResearch. Full-size immunoblot images are provided in fig. S19.

Cho Y., Kim H.S., Kang D., Kim H., Lee N., Yun J., Kim Y.J., Lee K.M., Kim J.H., Kim H.R., Hwang Y.I., Jo C.H, & Kim J.H. (2021). CTRP3 exacerbates tendinopathy by dysregulating tendon stem cell differentiation and altering extracellular matrix composition. Science Advances, 7(47), eabg6069.

+ Open protocol

+ Expand

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were lysed in HEPES-based lysis buffer (20 mM HEPES (pH 7.8), 500 mM NaCl, 5 mM MgCl2, 5 mM KCl, 0.1% deoxycholate, 0.5% Nonidet-P40, 10 mg/ml aprotinin, 10 mg/ml leupeptin, 200 mM Na₃VO₄, 1 mM phenylmethanesulphonylfluoride and 100 mM NaF). In all, 30–50 μg of protein lysate was separated by SDS-PAGE and was transferred onto nitrocellulose membranes. Anti-β-actin (C4), NRAS (F155) and p21 (N20) antibodies were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). P19-ARF antibody (#ab80) was from Abcam (Cambridge, UK). Antibodies directed against P-ERK1/2 (Thr202/Tyr204, #9101), P-AKT (Ser473, #4060), P-p53 (Ser18, #9284) and γ-H2AX (#2577) were purchased from Cell Signaling Technologies (Danvers, MA, USA). Anti-vinculin antibody (#V-9131) was acquired from Sigma-Aldrich (Heidelberg, Germany). P53 antibody was generated from hybridoma supernatant and was supplied by T. Stiewe (Institute of Molecular Biology and Tumor Research, University of Marburg, Germany). Generally, the presented protein blots are representative for 2–3 independent experiments.

Leikam C., Hufnagel A.L., Otto C., Murphy D.J., Mühling B., Kneitz S., Nanda I., Schmid M., Wagner T.U., Haferkamp S., Bröcker E.B., Schartl M, & Meierjohann S. (2015). In vitro evidence for senescent multinucleated melanocytes as a source for tumor-initiating cells. Cell Death & Disease, 6(4), e1711-.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!